Modeling of Endothelial Calcium Responses within a Microfluidic Generator of Spatio-Temporal ATP and Shear Stress Signals
Intracellular calcium dynamics play essential roles in the proper functioning of cellular activities. It is a well known important chemosensing and mechanosensing process regulated by the spatio-temporal microenvironment. Nevertheless, how spatio-temporal biochemical and biomechanical stimuli affect...
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MDPI AG
2021-02-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/12/2/161 |
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| author | Yong-Jiang Li Miao Yu Chun-Dong Xue Hai-Jun Zhang Guo-Zhen Wang Xiao-Ming Chen Kai-Rong Qin |
| author_facet | Yong-Jiang Li Miao Yu Chun-Dong Xue Hai-Jun Zhang Guo-Zhen Wang Xiao-Ming Chen Kai-Rong Qin |
| author_sort | Yong-Jiang Li |
| collection | DOAJ |
| description | Intracellular calcium dynamics play essential roles in the proper functioning of cellular activities. It is a well known important chemosensing and mechanosensing process regulated by the spatio-temporal microenvironment. Nevertheless, how spatio-temporal biochemical and biomechanical stimuli affect calcium dynamics is not fully understood and the underlying regulation mechanism remains missing. Herein, based on a developed microfluidic generator of biochemical and biomechanical signals, we theoretically analyzed the generation of spatio-temporal ATP and shear stress signals within the microfluidic platform and investigated the effect of spatial combination of ATP and shear stress stimuli on the intracellular calcium dynamics. The simulation results demonstrate the capacity and flexibility of the microfluidic system in generating spatio-temporal ATP and shear stress. Along the transverse direction of the microchannel, dynamic ATP signals of distinct amplitudes coupled with identical shear stress are created, which induce the spatio-temporal diversity in calcium responses. Interestingly, to the multiple combinations of stimuli, the intracellular calcium dynamics reveal two main modes: unimodal and oscillatory modes, showing significant dependence on the features of the spatio-temporal ATP and shear stress stimuli. The present study provides essential information for controlling calcium dynamics by regulating spatio-temporal biochemical and biomechanical stimuli, which shows the potential in directing cellular activities and understanding the occurrence and development of disease. |
| first_indexed | 2024-03-09T05:17:31Z |
| format | Article |
| id | doaj.art-cefc6aefcb234672a0bc73655f5feb2a |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-09T05:17:31Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-cefc6aefcb234672a0bc73655f5feb2a2023-12-03T12:44:39ZengMDPI AGMicromachines2072-666X2021-02-0112216110.3390/mi12020161Modeling of Endothelial Calcium Responses within a Microfluidic Generator of Spatio-Temporal ATP and Shear Stress SignalsYong-Jiang Li0Miao Yu1Chun-Dong Xue2Hai-Jun Zhang3Guo-Zhen Wang4Xiao-Ming Chen5Kai-Rong Qin6School of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, Dalian 116024, ChinaSchool of Biomedical Engineering, Dalian University of Technology, Dalian 116024, ChinaSchool of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, Dalian 116024, ChinaSchool of Biomedical Engineering, Dalian University of Technology, Dalian 116024, ChinaSchool of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, Dalian 116024, ChinaSchool of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, Dalian 116024, ChinaSchool of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, Dalian 116024, ChinaIntracellular calcium dynamics play essential roles in the proper functioning of cellular activities. It is a well known important chemosensing and mechanosensing process regulated by the spatio-temporal microenvironment. Nevertheless, how spatio-temporal biochemical and biomechanical stimuli affect calcium dynamics is not fully understood and the underlying regulation mechanism remains missing. Herein, based on a developed microfluidic generator of biochemical and biomechanical signals, we theoretically analyzed the generation of spatio-temporal ATP and shear stress signals within the microfluidic platform and investigated the effect of spatial combination of ATP and shear stress stimuli on the intracellular calcium dynamics. The simulation results demonstrate the capacity and flexibility of the microfluidic system in generating spatio-temporal ATP and shear stress. Along the transverse direction of the microchannel, dynamic ATP signals of distinct amplitudes coupled with identical shear stress are created, which induce the spatio-temporal diversity in calcium responses. Interestingly, to the multiple combinations of stimuli, the intracellular calcium dynamics reveal two main modes: unimodal and oscillatory modes, showing significant dependence on the features of the spatio-temporal ATP and shear stress stimuli. The present study provides essential information for controlling calcium dynamics by regulating spatio-temporal biochemical and biomechanical stimuli, which shows the potential in directing cellular activities and understanding the occurrence and development of disease.https://www.mdpi.com/2072-666X/12/2/161intracellular calcium dynamicsspatio-temporal signalsATP and shear stress stimulitheoretical modelingendothelial cells |
| spellingShingle | Yong-Jiang Li Miao Yu Chun-Dong Xue Hai-Jun Zhang Guo-Zhen Wang Xiao-Ming Chen Kai-Rong Qin Modeling of Endothelial Calcium Responses within a Microfluidic Generator of Spatio-Temporal ATP and Shear Stress Signals Micromachines intracellular calcium dynamics spatio-temporal signals ATP and shear stress stimuli theoretical modeling endothelial cells |
| title | Modeling of Endothelial Calcium Responses within a Microfluidic Generator of Spatio-Temporal ATP and Shear Stress Signals |
| title_full | Modeling of Endothelial Calcium Responses within a Microfluidic Generator of Spatio-Temporal ATP and Shear Stress Signals |
| title_fullStr | Modeling of Endothelial Calcium Responses within a Microfluidic Generator of Spatio-Temporal ATP and Shear Stress Signals |
| title_full_unstemmed | Modeling of Endothelial Calcium Responses within a Microfluidic Generator of Spatio-Temporal ATP and Shear Stress Signals |
| title_short | Modeling of Endothelial Calcium Responses within a Microfluidic Generator of Spatio-Temporal ATP and Shear Stress Signals |
| title_sort | modeling of endothelial calcium responses within a microfluidic generator of spatio temporal atp and shear stress signals |
| topic | intracellular calcium dynamics spatio-temporal signals ATP and shear stress stimuli theoretical modeling endothelial cells |
| url | https://www.mdpi.com/2072-666X/12/2/161 |
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