The effect of carbon monoxide on meiotic maturation of porcine oocytes
Oxidative stress impairs the correct course of meiotic maturation, and it is known that the oocytes are exposed to increased oxidative stress during meiotic maturation in in vitro conditions. Thus, reduction of oxidative stress can lead to improved quality of cultured oocytes. The gasotransmitter ca...
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PeerJ Inc.
2021-03-01
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| author | David Němeček Eva Chmelikova Jaroslav Petr Tomas Kott Markéta Sedmíková |
| author_facet | David Němeček Eva Chmelikova Jaroslav Petr Tomas Kott Markéta Sedmíková |
| author_sort | David Němeček |
| collection | DOAJ |
| description | Oxidative stress impairs the correct course of meiotic maturation, and it is known that the oocytes are exposed to increased oxidative stress during meiotic maturation in in vitro conditions. Thus, reduction of oxidative stress can lead to improved quality of cultured oocytes. The gasotransmitter carbon monoxide (CO) has a cytoprotective effect in somatic cells. The CO is produced in cells by the enzyme heme oxygenase (HO) and the heme oxygenase/carbon monoxide (HO/CO) pathway has been shown to have an antioxidant effect in somatic cells. It has not yet been investigated whether the CO has an antioxidant effect in oocytes as well. We assessed the level of expression of HO mRNA, using reverse transcription polymerase chain reaction. The HO protein localization was evaluated by the immunocytochemical method. The influence of CO or HO inhibition on meiotic maturation was evaluated in oocytes cultured in a culture medium containing CO donor (CORM-2 or CORM-A1) or HO inhibitor Zn-protoporphyrin IX (Zn-PP IX). Detection of reactive oxygen species (ROS) was performed using the oxidant-sensing probe 2′,7′-dichlorodihydrofluorescein diacetate. We demonstrated the expression of mRNA and proteins of both HO isoforms in porcine oocytes during meiotic maturation. The inhibition of HO enzymes by Zn-PP IX did not affect meiotic maturation. CO delivered by CORM-2 or CORM-A1 donors led to a reduction in the level of ROS in the oocytes during meiotic maturation. However, exogenously delivered CO also inhibited meiotic maturation, especially at higher concentrations. In summary, the CO signaling molecule has antioxidant properties in porcine oocytes and may also be involved in the regulation of meiotic maturation. |
| first_indexed | 2024-03-09T07:53:58Z |
| format | Article |
| id | doaj.art-ceffb4f6c38f4c35835c917ec0cb8218 |
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| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:53:58Z |
| publishDate | 2021-03-01 |
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| spelling | doaj.art-ceffb4f6c38f4c35835c917ec0cb82182023-12-03T01:22:30ZengPeerJ Inc.PeerJ2167-83592021-03-019e1063610.7717/peerj.10636The effect of carbon monoxide on meiotic maturation of porcine oocytesDavid Němeček0Eva Chmelikova1Jaroslav Petr2Tomas Kott3Markéta Sedmíková4Department of Veterinary Sciences, Czech University of Life Sciences, Prague, Czech RepublicDepartment of Veterinary Sciences, Czech University of Life Sciences, Prague, Czech RepublicInstitute of Animal Science, Uhřiněves, Czech RepublicInstitute of Animal Science, Uhřiněves, Czech RepublicDepartment of Veterinary Sciences, Czech University of Life Sciences, Prague, Czech RepublicOxidative stress impairs the correct course of meiotic maturation, and it is known that the oocytes are exposed to increased oxidative stress during meiotic maturation in in vitro conditions. Thus, reduction of oxidative stress can lead to improved quality of cultured oocytes. The gasotransmitter carbon monoxide (CO) has a cytoprotective effect in somatic cells. The CO is produced in cells by the enzyme heme oxygenase (HO) and the heme oxygenase/carbon monoxide (HO/CO) pathway has been shown to have an antioxidant effect in somatic cells. It has not yet been investigated whether the CO has an antioxidant effect in oocytes as well. We assessed the level of expression of HO mRNA, using reverse transcription polymerase chain reaction. The HO protein localization was evaluated by the immunocytochemical method. The influence of CO or HO inhibition on meiotic maturation was evaluated in oocytes cultured in a culture medium containing CO donor (CORM-2 or CORM-A1) or HO inhibitor Zn-protoporphyrin IX (Zn-PP IX). Detection of reactive oxygen species (ROS) was performed using the oxidant-sensing probe 2′,7′-dichlorodihydrofluorescein diacetate. We demonstrated the expression of mRNA and proteins of both HO isoforms in porcine oocytes during meiotic maturation. The inhibition of HO enzymes by Zn-PP IX did not affect meiotic maturation. CO delivered by CORM-2 or CORM-A1 donors led to a reduction in the level of ROS in the oocytes during meiotic maturation. However, exogenously delivered CO also inhibited meiotic maturation, especially at higher concentrations. In summary, the CO signaling molecule has antioxidant properties in porcine oocytes and may also be involved in the regulation of meiotic maturation.https://peerj.com/articles/10636.pdfOocyteMeiotic maturationHeme oxygenaseCarbon monoxideOxidative stress |
| spellingShingle | David Němeček Eva Chmelikova Jaroslav Petr Tomas Kott Markéta Sedmíková The effect of carbon monoxide on meiotic maturation of porcine oocytes PeerJ Oocyte Meiotic maturation Heme oxygenase Carbon monoxide Oxidative stress |
| title | The effect of carbon monoxide on meiotic maturation of porcine oocytes |
| title_full | The effect of carbon monoxide on meiotic maturation of porcine oocytes |
| title_fullStr | The effect of carbon monoxide on meiotic maturation of porcine oocytes |
| title_full_unstemmed | The effect of carbon monoxide on meiotic maturation of porcine oocytes |
| title_short | The effect of carbon monoxide on meiotic maturation of porcine oocytes |
| title_sort | effect of carbon monoxide on meiotic maturation of porcine oocytes |
| topic | Oocyte Meiotic maturation Heme oxygenase Carbon monoxide Oxidative stress |
| url | https://peerj.com/articles/10636.pdf |
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