Micro-Raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells.
We report here results of a single-cell Raman spectroscopy study of stress effects induced by silver nanoparticles in human mesenchymal stem cells (hMSCs). A high-sensitivity, high-resolution Raman Tweezers set-up has been used to monitor nanoparticle-induced biochemical changes in optically-trapped...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3325966?pdf=render |
| _version_ | 1818202111523946496 |
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| author | Aseefhali Bankapur R Sagar Krishnamurthy Elsa Zachariah Chidangil Santhosh Basavaraj Chougule Bhavishna Praveen Manna Valiathan Deepak Mathur |
| author_facet | Aseefhali Bankapur R Sagar Krishnamurthy Elsa Zachariah Chidangil Santhosh Basavaraj Chougule Bhavishna Praveen Manna Valiathan Deepak Mathur |
| author_sort | Aseefhali Bankapur |
| collection | DOAJ |
| description | We report here results of a single-cell Raman spectroscopy study of stress effects induced by silver nanoparticles in human mesenchymal stem cells (hMSCs). A high-sensitivity, high-resolution Raman Tweezers set-up has been used to monitor nanoparticle-induced biochemical changes in optically-trapped single cells. Our micro-Raman spectroscopic study reveals that hMSCs treated with silver nanoparticles undergo oxidative stress at doping levels in excess of 2 µg/ml, with results of a statistical analysis of Raman spectra suggesting that the induced stress becomes more dominant at nanoparticle concentration levels above 3 µg/ml. |
| first_indexed | 2024-12-12T03:04:15Z |
| format | Article |
| id | doaj.art-cf002c4fdc7840709133a2381e759253 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T03:04:15Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-cf002c4fdc7840709133a2381e7592532022-12-22T00:40:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0174e3507510.1371/journal.pone.0035075Micro-Raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells.Aseefhali BankapurR Sagar KrishnamurthyElsa ZachariahChidangil SanthoshBasavaraj ChouguleBhavishna PraveenManna ValiathanDeepak MathurWe report here results of a single-cell Raman spectroscopy study of stress effects induced by silver nanoparticles in human mesenchymal stem cells (hMSCs). A high-sensitivity, high-resolution Raman Tweezers set-up has been used to monitor nanoparticle-induced biochemical changes in optically-trapped single cells. Our micro-Raman spectroscopic study reveals that hMSCs treated with silver nanoparticles undergo oxidative stress at doping levels in excess of 2 µg/ml, with results of a statistical analysis of Raman spectra suggesting that the induced stress becomes more dominant at nanoparticle concentration levels above 3 µg/ml.http://europepmc.org/articles/PMC3325966?pdf=render |
| spellingShingle | Aseefhali Bankapur R Sagar Krishnamurthy Elsa Zachariah Chidangil Santhosh Basavaraj Chougule Bhavishna Praveen Manna Valiathan Deepak Mathur Micro-Raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells. PLoS ONE |
| title | Micro-Raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells. |
| title_full | Micro-Raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells. |
| title_fullStr | Micro-Raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells. |
| title_full_unstemmed | Micro-Raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells. |
| title_short | Micro-Raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells. |
| title_sort | micro raman spectroscopy of silver nanoparticle induced stress on optically trapped stem cells |
| url | http://europepmc.org/articles/PMC3325966?pdf=render |
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