Effects of equol on apoptosis and proliferation of osteoblast through PI3K/AKT pathway in high glucose and high fat environment
Objective To explore the effect and mechanism of equol(Eq) on the proliferation and apoptosis of osteoblasts in high-glucose and high-fat environment. Methods High-glucose and high-fat model of ROS17/2.8 osteoblasts was established, and cells were divided into the control group (C group) and the mod...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Army Medical University
2023-02-01
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| Series: | 陆军军医大学学报 |
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| Online Access: | http://aammt.tmmu.edu.cn/Upload/rhtml/202207184.htm |
| _version_ | 1797894153020899328 |
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| author | LI Shuo NI Xiangmin XU Zhe ZHU Wenyi LIANG Xinyu WANG Jian |
| author_facet | LI Shuo NI Xiangmin XU Zhe ZHU Wenyi LIANG Xinyu WANG Jian |
| author_sort | LI Shuo |
| collection | DOAJ |
| description | Objective To explore the effect and mechanism of equol(Eq) on the proliferation and apoptosis of osteoblasts in high-glucose and high-fat environment. Methods High-glucose and high-fat model of ROS17/2.8 osteoblasts was established, and cells were divided into the control group (C group) and the model group (M group), the Eq-7 group (1×10-7mol/L Eq), the Eq-6 group(1×10-6mol/L Eq) and the Eq-5 group(10-5mol/L Eq). CCK-8 assay and HE staining was used to detect the viability and morphology of osteoblasts; Flow cytometry was used to test the apoptosis and cell cycle; Western blotting was used to detect the phosphorylation of PI3K/AKT signaling pathway and expression of associated proteins of osteoblasts. PI3K inhibitor was added to confirm the role of PI3K/AKT signaling pathway in the osteoprotective effect of Eq. Results Compared with the C group, the cell structure of the M group was significantly damaged, the cell survival rate, proportion of S phase of cell cycle, the degree of phosphorylation of PI3K/AKT pathway, the expression of Cyclin D1 and Bcl-2 in the M group decreased, while cell inhibition rate, cell apoptosis, proportion of G1 phase of cell cycle, the expression of Bax and cleaved caspase-3 of the M group increased (P < 0.05); Compared with the M group, the Eq groups had increased cell survival rate, decreased cell inhibition rate, enhanced fraction of osteoblast in the S phase and decreased osteoblast apoptosis rate(P < 0.05). Eq treatment enhanced the phosphorylation of PI3K/AKT pathway of osteoblasts and increased expression level of Cyclin D1 and Bcl-2 protein and decreased expression of Bax and cleaved caspase-3 (P < 0.05). The intervention effect of Eq enhanced with the increase of concentration of Eq. However, this regulatory effect of Eq almost disappeared after co-administration of PI3K inhibitor. Conclusion Eq can reduce the apoptosis and promote the proliferation of osteoblasts in the high-glucose and high-fat environment by activating the PI3K/AKT pathway.
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| first_indexed | 2024-04-10T07:04:18Z |
| format | Article |
| id | doaj.art-cf031178a9e14f73b3906b478fd8d53f |
| institution | Directory Open Access Journal |
| issn | 2097-0927 |
| language | zho |
| last_indexed | 2024-04-10T07:04:18Z |
| publishDate | 2023-02-01 |
| publisher | Editorial Office of Journal of Army Medical University |
| record_format | Article |
| series | 陆军军医大学学报 |
| spelling | doaj.art-cf031178a9e14f73b3906b478fd8d53f2023-02-27T11:17:00ZzhoEditorial Office of Journal of Army Medical University陆军军医大学学报2097-09272023-02-0145435035710.16016/j.2097-0927.202207184Effects of equol on apoptosis and proliferation of osteoblast through PI3K/AKT pathway in high glucose and high fat environmentLI Shuo0NI Xiangmin1XU Zhe2ZHU Wenyi3LIANG Xinyu4WANG Jian5Department of Nutrition, Second Affiliated Hospital, Army Medical University(Third Military Medical University), Chongqing, 400037, ChinaDepartment of Nutrition, Second Affiliated Hospital, Army Medical University(Third Military Medical University), Chongqing, 400037, ChinaDepartment of Nutrition, Second Affiliated Hospital, Army Medical University(Third Military Medical University), Chongqing, 400037, ChinaDepartment of Nutrition, Second Affiliated Hospital, Army Medical University(Third Military Medical University), Chongqing, 400037, ChinaDepartment of Nutrition, Second Affiliated Hospital, Army Medical University(Third Military Medical University), Chongqing, 400037, ChinaDepartment of Nutrition, Second Affiliated Hospital, Army Medical University(Third Military Medical University), Chongqing, 400037, ChinaObjective To explore the effect and mechanism of equol(Eq) on the proliferation and apoptosis of osteoblasts in high-glucose and high-fat environment. Methods High-glucose and high-fat model of ROS17/2.8 osteoblasts was established, and cells were divided into the control group (C group) and the model group (M group), the Eq-7 group (1×10-7mol/L Eq), the Eq-6 group(1×10-6mol/L Eq) and the Eq-5 group(10-5mol/L Eq). CCK-8 assay and HE staining was used to detect the viability and morphology of osteoblasts; Flow cytometry was used to test the apoptosis and cell cycle; Western blotting was used to detect the phosphorylation of PI3K/AKT signaling pathway and expression of associated proteins of osteoblasts. PI3K inhibitor was added to confirm the role of PI3K/AKT signaling pathway in the osteoprotective effect of Eq. Results Compared with the C group, the cell structure of the M group was significantly damaged, the cell survival rate, proportion of S phase of cell cycle, the degree of phosphorylation of PI3K/AKT pathway, the expression of Cyclin D1 and Bcl-2 in the M group decreased, while cell inhibition rate, cell apoptosis, proportion of G1 phase of cell cycle, the expression of Bax and cleaved caspase-3 of the M group increased (P < 0.05); Compared with the M group, the Eq groups had increased cell survival rate, decreased cell inhibition rate, enhanced fraction of osteoblast in the S phase and decreased osteoblast apoptosis rate(P < 0.05). Eq treatment enhanced the phosphorylation of PI3K/AKT pathway of osteoblasts and increased expression level of Cyclin D1 and Bcl-2 protein and decreased expression of Bax and cleaved caspase-3 (P < 0.05). The intervention effect of Eq enhanced with the increase of concentration of Eq. However, this regulatory effect of Eq almost disappeared after co-administration of PI3K inhibitor. Conclusion Eq can reduce the apoptosis and promote the proliferation of osteoblasts in the high-glucose and high-fat environment by activating the PI3K/AKT pathway. http://aammt.tmmu.edu.cn/Upload/rhtml/202207184.htmequolhigh-glucose and high-fatosteoblastsapoptosisproliferation |
| spellingShingle | LI Shuo NI Xiangmin XU Zhe ZHU Wenyi LIANG Xinyu WANG Jian Effects of equol on apoptosis and proliferation of osteoblast through PI3K/AKT pathway in high glucose and high fat environment 陆军军医大学学报 equol high-glucose and high-fat osteoblasts apoptosis proliferation |
| title | Effects of equol on apoptosis and proliferation of osteoblast through PI3K/AKT pathway in high glucose and high fat environment |
| title_full | Effects of equol on apoptosis and proliferation of osteoblast through PI3K/AKT pathway in high glucose and high fat environment |
| title_fullStr | Effects of equol on apoptosis and proliferation of osteoblast through PI3K/AKT pathway in high glucose and high fat environment |
| title_full_unstemmed | Effects of equol on apoptosis and proliferation of osteoblast through PI3K/AKT pathway in high glucose and high fat environment |
| title_short | Effects of equol on apoptosis and proliferation of osteoblast through PI3K/AKT pathway in high glucose and high fat environment |
| title_sort | effects of equol on apoptosis and proliferation of osteoblast through pi3k akt pathway in high glucose and high fat environment |
| topic | equol high-glucose and high-fat osteoblasts apoptosis proliferation |
| url | http://aammt.tmmu.edu.cn/Upload/rhtml/202207184.htm |
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