Protocatechuic acid inhibits proliferation, migration and inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes
AbstractRheumatoid arthritis (RA) is a chronic joint inflammatory disease that is closely associated with dysregulation of fibroblast-like synoviocytes (FLSs). Protocatechuic acid (PCA), a phenolic compound of anthocyanins, has been proven to possess anti-inflammatory activity. However, the role of...
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Taylor & Francis Group
2020-01-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2020.1776307 |
| _version_ | 1797788833460256768 |
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| author | Huiqiang Wu Jing Wang Qing Zhao Yanjie Ding Bingyi Zhang Lingli Kong |
| author_facet | Huiqiang Wu Jing Wang Qing Zhao Yanjie Ding Bingyi Zhang Lingli Kong |
| author_sort | Huiqiang Wu |
| collection | DOAJ |
| description | AbstractRheumatoid arthritis (RA) is a chronic joint inflammatory disease that is closely associated with dysregulation of fibroblast-like synoviocytes (FLSs). Protocatechuic acid (PCA), a phenolic compound of anthocyanins, has been proven to possess anti-inflammatory activity. However, the role of PCA in RA has not been investigated. In the present study, we aimed to explore the effects of PCA on the RA-FLSs. The results showed that PCA suppressed the proliferation, invasion, and migration of RA-FLSs in a dose-dependent manner. PCA treatment also inhibited the expressions of matrix metalloproteinase (MMP)-3 and MMP-13, as well as the secretion of inflammatory cytokines including TNF-α, IL-1β, IL-6 in RA-FLSs. Moreover, cell apoptosis of RA-FLSs was significantly induced by PCA treatment. PCA was found to repress the activation of NF-κB signalling, which was evidenced by the decreased expression of p-p65 and increased expression of IκBα. Furthermore, PCA significantly decreased the phosphorylation levels of Akt and mTOR in RA-FLSs. In conclusion, the results indicated that PCA exhibited an inhibitory effect on RA-FLSs via inhibiting the NF-κB and Akt/mTOR signalling pathways. These findings supported the concept that PCA might be a therapeutic agent for RA treatment. |
| first_indexed | 2024-03-13T01:42:06Z |
| format | Article |
| id | doaj.art-cf05839604844a29b3d401035fb3a840 |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-03-13T01:42:06Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
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| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-cf05839604844a29b3d401035fb3a8402023-07-03T14:04:55ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2020-01-0148196997610.1080/21691401.2020.1776307Protocatechuic acid inhibits proliferation, migration and inflammatory response in rheumatoid arthritis fibroblast-like synoviocytesHuiqiang Wu0Jing Wang1Qing Zhao2Yanjie Ding3Bingyi Zhang4Lingli Kong5Department of Rheumatology and Immunology, Huaihe Hospital, Henan University, Kaifeng, People’s Republic of ChinaDepartment of Rheumatology and Immunology, Huaihe Hospital, Henan University, Kaifeng, People’s Republic of ChinaDepartment of Rheumatology and Immunology, Huaihe Hospital, Henan University, Kaifeng, People’s Republic of ChinaDepartment of Rheumatology and Immunology, Huaihe Hospital, Henan University, Kaifeng, People’s Republic of ChinaDepartment of Rheumatology and Immunology, Huaihe Hospital, Henan University, Kaifeng, People’s Republic of ChinaDepartment of Rheumatology and Immunology, Huaihe Hospital, Henan University, Kaifeng, People’s Republic of ChinaAbstractRheumatoid arthritis (RA) is a chronic joint inflammatory disease that is closely associated with dysregulation of fibroblast-like synoviocytes (FLSs). Protocatechuic acid (PCA), a phenolic compound of anthocyanins, has been proven to possess anti-inflammatory activity. However, the role of PCA in RA has not been investigated. In the present study, we aimed to explore the effects of PCA on the RA-FLSs. The results showed that PCA suppressed the proliferation, invasion, and migration of RA-FLSs in a dose-dependent manner. PCA treatment also inhibited the expressions of matrix metalloproteinase (MMP)-3 and MMP-13, as well as the secretion of inflammatory cytokines including TNF-α, IL-1β, IL-6 in RA-FLSs. Moreover, cell apoptosis of RA-FLSs was significantly induced by PCA treatment. PCA was found to repress the activation of NF-κB signalling, which was evidenced by the decreased expression of p-p65 and increased expression of IκBα. Furthermore, PCA significantly decreased the phosphorylation levels of Akt and mTOR in RA-FLSs. In conclusion, the results indicated that PCA exhibited an inhibitory effect on RA-FLSs via inhibiting the NF-κB and Akt/mTOR signalling pathways. These findings supported the concept that PCA might be a therapeutic agent for RA treatment.https://www.tandfonline.com/doi/10.1080/21691401.2020.1776307Rheumatoid arthritis (RA)fibroblast-like synoviocytes (FLSs)PCAinflammationNF-κB signalling pathwayAkt/mTOR pathway |
| spellingShingle | Huiqiang Wu Jing Wang Qing Zhao Yanjie Ding Bingyi Zhang Lingli Kong Protocatechuic acid inhibits proliferation, migration and inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes Artificial Cells, Nanomedicine, and Biotechnology Rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLSs) PCA inflammation NF-κB signalling pathway Akt/mTOR pathway |
| title | Protocatechuic acid inhibits proliferation, migration and inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes |
| title_full | Protocatechuic acid inhibits proliferation, migration and inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes |
| title_fullStr | Protocatechuic acid inhibits proliferation, migration and inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes |
| title_full_unstemmed | Protocatechuic acid inhibits proliferation, migration and inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes |
| title_short | Protocatechuic acid inhibits proliferation, migration and inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes |
| title_sort | protocatechuic acid inhibits proliferation migration and inflammatory response in rheumatoid arthritis fibroblast like synoviocytes |
| topic | Rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLSs) PCA inflammation NF-κB signalling pathway Akt/mTOR pathway |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2020.1776307 |
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