Levels of IL-23/IL-17 Axis in Plasma and Gingival Tissue of Periodontitis Patients According to the New Classification
Background: Periodontitis (P) is a chronic inflammatory disease characterized by the destruction of periodontium support tissue generated by different immuno-inflammatory mechanisms, including the RANK/RANKL/OPG and the IL-23/IL-17 axis. Methods: The study was performed with healthy subjects (HS) an...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Online Access: | https://www.mdpi.com/2076-3417/12/16/8051 |
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| author | Ruth Rodríguez-Montaño Alondra del Carmen Ruiz-Gutiérrez Vianeth María del Carmen Martínez-Rodríguez Juan Ramón Gómez-Sandoval Juan Manuel Guzmán-Flores Julieta Sarai Becerra-Ruiz Ana Lourdes Zamora-Perez Celia Guerrero-Velázquez |
| author_facet | Ruth Rodríguez-Montaño Alondra del Carmen Ruiz-Gutiérrez Vianeth María del Carmen Martínez-Rodríguez Juan Ramón Gómez-Sandoval Juan Manuel Guzmán-Flores Julieta Sarai Becerra-Ruiz Ana Lourdes Zamora-Perez Celia Guerrero-Velázquez |
| author_sort | Ruth Rodríguez-Montaño |
| collection | DOAJ |
| description | Background: Periodontitis (P) is a chronic inflammatory disease characterized by the destruction of periodontium support tissue generated by different immuno-inflammatory mechanisms, including the RANK/RANKL/OPG and the IL-23/IL-17 axis. Methods: The study was performed with healthy subjects (HS) and patients with periodontitis. Plasma samples were obtained from peripheral blood and the gingival tissue (GT) during periodontal surgery. The ELISA technique was used to evaluate the levels of IL-23, IL-17A, IL-23R, and IL-17RA. Results: In the plasma, a significant decrease in IL-17A was observed in patients with periodontitis than HS. In the GT, IL-23, IL-17A, and IL-17RA levels were increased in periodontitis patients; on the contrary, IL-23R levels were decreased in periodontitis patients when compared with HS. Finally, several positive correlations were found: soluble IL-17RA (sIL-17RA) levels in plasma between the percentage of radiographic bone loss (RBL%), and IL-23 with IL-17A in gingival tissue. Conclusions: The detection of the IL-23/IL-17A axis in gingival tissue and plasma provides us with more information on the behavior of this axis in a localized way in the periodontal microenvironment, in contrast to the systemic levels evaluated according to the new classification of periodontitis. |
| first_indexed | 2024-03-09T11:57:57Z |
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| language | English |
| last_indexed | 2024-03-09T11:57:57Z |
| publishDate | 2022-08-01 |
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| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-cf083cb16f8b408686f660499d0e41dc2023-11-30T23:07:39ZengMDPI AGApplied Sciences2076-34172022-08-011216805110.3390/app12168051Levels of IL-23/IL-17 Axis in Plasma and Gingival Tissue of Periodontitis Patients According to the New ClassificationRuth Rodríguez-Montaño0Alondra del Carmen Ruiz-Gutiérrez1Vianeth María del Carmen Martínez-Rodríguez2Juan Ramón Gómez-Sandoval3Juan Manuel Guzmán-Flores4Julieta Sarai Becerra-Ruiz5Ana Lourdes Zamora-Perez6Celia Guerrero-Velázquez7Department of Integral Dental Clinics, Institute of Research in Dentistry, University Center of Health Sciences, University of Guadalajara, Guadalajara 44340, Jalisco, MexicoSpecialty in Periodontology, Department of Integral Dental Clinics, University Center of Health Sciences, University of Guadalajara, Guadalajara 44340, Jalisco, MexicoSpecialty in Periodontology, Department of Integral Dental Clinics, University Center of Health Sciences, University of Guadalajara, Guadalajara 44340, Jalisco, MexicoDepartment of Integral Dental Clinics, Institute of Research in Dentistry, University Center of Health Sciences, University of Guadalajara, Guadalajara 44340, Jalisco, MexicoInstitute of Research of Bioscience, University Center of Los Altos, University of Guadalajara, Tepatitlán de Morelos 47600, Jalisco, MexicoInstitute of Research of Bioscience, University Center of Los Altos, University of Guadalajara, Tepatitlán de Morelos 47600, Jalisco, MexicoDepartment of Integral Dental Clinics, Institute of Research in Dentistry, University Center of Health Sciences, University of Guadalajara, Guadalajara 44340, Jalisco, MexicoDepartment of Integral Dental Clinics, Institute of Research in Dentistry, University Center of Health Sciences, University of Guadalajara, Guadalajara 44340, Jalisco, MexicoBackground: Periodontitis (P) is a chronic inflammatory disease characterized by the destruction of periodontium support tissue generated by different immuno-inflammatory mechanisms, including the RANK/RANKL/OPG and the IL-23/IL-17 axis. Methods: The study was performed with healthy subjects (HS) and patients with periodontitis. Plasma samples were obtained from peripheral blood and the gingival tissue (GT) during periodontal surgery. The ELISA technique was used to evaluate the levels of IL-23, IL-17A, IL-23R, and IL-17RA. Results: In the plasma, a significant decrease in IL-17A was observed in patients with periodontitis than HS. In the GT, IL-23, IL-17A, and IL-17RA levels were increased in periodontitis patients; on the contrary, IL-23R levels were decreased in periodontitis patients when compared with HS. Finally, several positive correlations were found: soluble IL-17RA (sIL-17RA) levels in plasma between the percentage of radiographic bone loss (RBL%), and IL-23 with IL-17A in gingival tissue. Conclusions: The detection of the IL-23/IL-17A axis in gingival tissue and plasma provides us with more information on the behavior of this axis in a localized way in the periodontal microenvironment, in contrast to the systemic levels evaluated according to the new classification of periodontitis.https://www.mdpi.com/2076-3417/12/16/8051Interleukin-23Interleukin-17Interleukin-23 receptorInterleukin-17 receptorperiodontitis |
| spellingShingle | Ruth Rodríguez-Montaño Alondra del Carmen Ruiz-Gutiérrez Vianeth María del Carmen Martínez-Rodríguez Juan Ramón Gómez-Sandoval Juan Manuel Guzmán-Flores Julieta Sarai Becerra-Ruiz Ana Lourdes Zamora-Perez Celia Guerrero-Velázquez Levels of IL-23/IL-17 Axis in Plasma and Gingival Tissue of Periodontitis Patients According to the New Classification Applied Sciences Interleukin-23 Interleukin-17 Interleukin-23 receptor Interleukin-17 receptor periodontitis |
| title | Levels of IL-23/IL-17 Axis in Plasma and Gingival Tissue of Periodontitis Patients According to the New Classification |
| title_full | Levels of IL-23/IL-17 Axis in Plasma and Gingival Tissue of Periodontitis Patients According to the New Classification |
| title_fullStr | Levels of IL-23/IL-17 Axis in Plasma and Gingival Tissue of Periodontitis Patients According to the New Classification |
| title_full_unstemmed | Levels of IL-23/IL-17 Axis in Plasma and Gingival Tissue of Periodontitis Patients According to the New Classification |
| title_short | Levels of IL-23/IL-17 Axis in Plasma and Gingival Tissue of Periodontitis Patients According to the New Classification |
| title_sort | levels of il 23 il 17 axis in plasma and gingival tissue of periodontitis patients according to the new classification |
| topic | Interleukin-23 Interleukin-17 Interleukin-23 receptor Interleukin-17 receptor periodontitis |
| url | https://www.mdpi.com/2076-3417/12/16/8051 |
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