Acetylated Polysaccharides From <i>Pleurotus geesteranus</i> Alleviate Lung Injury Via Regulating NF-κB Signal Pathway
The present work investigated the anti-inflammatory, antioxidant, and lung protection effects of acetylated <i>Pleurotus geesteranus</i> polysaccharides (AcPPS) on acute lung injury (ALI) mice. The acetylation of AcPPS was successfully shown by the peaks of 1737 cm<sup>−1</sup&g...
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MDPI AG
2020-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/8/2810 |
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| author | Xinling Song Jianjun Zhang Jian Li Le Jia |
| author_facet | Xinling Song Jianjun Zhang Jian Li Le Jia |
| author_sort | Xinling Song |
| collection | DOAJ |
| description | The present work investigated the anti-inflammatory, antioxidant, and lung protection effects of acetylated <i>Pleurotus geesteranus</i> polysaccharides (AcPPS) on acute lung injury (ALI) mice. The acetylation of AcPPS was successfully shown by the peaks of 1737 cm<sup>−1</sup> and 1249 cm<sup>−1</sup> by FTIR. The animal experiments demonstrated that lung damage can be induced by zymosan. However, the supplementation of AcPPS had potential effects on reducing lung index, remitting inflammatory symptoms (TNF-α, IL-1β, and IL-6), inhibiting NF-κB signal pathway based on up-regulating the level of IκBα and down-regulating p-IκBα level by Western blotting and immunofluorescence assay, preventing oxidative stress (ROS, SOD, GSH-Px, CAT, T-AOC, and MDA), reducing lipid accumulation (TC, TG, LDL-C, HDL-C, and VLDL-C), and alleviating lung functions by histopathologic observation. These results demonstrated that AcPPS might be suitable for natural food for prevention or remission in ALI. |
| first_indexed | 2024-03-10T20:24:34Z |
| format | Article |
| id | doaj.art-cf0f2b14ce504df9be7bf15534f3889b |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T20:24:34Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-cf0f2b14ce504df9be7bf15534f3889b2023-11-19T21:57:36ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-04-01218281010.3390/ijms21082810Acetylated Polysaccharides From <i>Pleurotus geesteranus</i> Alleviate Lung Injury Via Regulating NF-κB Signal PathwayXinling Song0Jianjun Zhang1Jian Li2Le Jia3College of Food and Biological Engineering, Jimei University, Xiamen 361021, ChinaCollege of Life Science, Shandong Agricultural University, Taian 271018, ChinaCollege of Food and Biological Engineering, Jimei University, Xiamen 361021, ChinaCollege of Life Science, Shandong Agricultural University, Taian 271018, ChinaThe present work investigated the anti-inflammatory, antioxidant, and lung protection effects of acetylated <i>Pleurotus geesteranus</i> polysaccharides (AcPPS) on acute lung injury (ALI) mice. The acetylation of AcPPS was successfully shown by the peaks of 1737 cm<sup>−1</sup> and 1249 cm<sup>−1</sup> by FTIR. The animal experiments demonstrated that lung damage can be induced by zymosan. However, the supplementation of AcPPS had potential effects on reducing lung index, remitting inflammatory symptoms (TNF-α, IL-1β, and IL-6), inhibiting NF-κB signal pathway based on up-regulating the level of IκBα and down-regulating p-IκBα level by Western blotting and immunofluorescence assay, preventing oxidative stress (ROS, SOD, GSH-Px, CAT, T-AOC, and MDA), reducing lipid accumulation (TC, TG, LDL-C, HDL-C, and VLDL-C), and alleviating lung functions by histopathologic observation. These results demonstrated that AcPPS might be suitable for natural food for prevention or remission in ALI.https://www.mdpi.com/1422-0067/21/8/2810Acetylated polysaccharidesAcute lung injuryAntioxidant<i>Pleurotus geesteranus</i> |
| spellingShingle | Xinling Song Jianjun Zhang Jian Li Le Jia Acetylated Polysaccharides From <i>Pleurotus geesteranus</i> Alleviate Lung Injury Via Regulating NF-κB Signal Pathway International Journal of Molecular Sciences Acetylated polysaccharides Acute lung injury Antioxidant <i>Pleurotus geesteranus</i> |
| title | Acetylated Polysaccharides From <i>Pleurotus geesteranus</i> Alleviate Lung Injury Via Regulating NF-κB Signal Pathway |
| title_full | Acetylated Polysaccharides From <i>Pleurotus geesteranus</i> Alleviate Lung Injury Via Regulating NF-κB Signal Pathway |
| title_fullStr | Acetylated Polysaccharides From <i>Pleurotus geesteranus</i> Alleviate Lung Injury Via Regulating NF-κB Signal Pathway |
| title_full_unstemmed | Acetylated Polysaccharides From <i>Pleurotus geesteranus</i> Alleviate Lung Injury Via Regulating NF-κB Signal Pathway |
| title_short | Acetylated Polysaccharides From <i>Pleurotus geesteranus</i> Alleviate Lung Injury Via Regulating NF-κB Signal Pathway |
| title_sort | acetylated polysaccharides from i pleurotus geesteranus i alleviate lung injury via regulating nf κb signal pathway |
| topic | Acetylated polysaccharides Acute lung injury Antioxidant <i>Pleurotus geesteranus</i> |
| url | https://www.mdpi.com/1422-0067/21/8/2810 |
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