Identification of <i>Streptococcus thermophilus</i> Genes Specifically Expressed under Simulated Human Digestive Conditions Using R-IVET Technology
Despite promising health effects, the probiotic status of <i>Streptococcus thermophilus,</i> a lactic acid bacterium widely used in dairy industry, requires further documentation of its physiological status during human gastrointestinal passage. This study aimed to apply recombinant-base...
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MDPI AG
2021-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/6/1113 |
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| author | Ophélie Uriot Mounira Kebouchi Emilie Lorson Wessam Galia Sylvain Denis Sandrine Chalancon Zeeshan Hafeez Emeline Roux Magali Genay Stéphanie Blanquet-Diot Annie Dary-Mourot |
| author_facet | Ophélie Uriot Mounira Kebouchi Emilie Lorson Wessam Galia Sylvain Denis Sandrine Chalancon Zeeshan Hafeez Emeline Roux Magali Genay Stéphanie Blanquet-Diot Annie Dary-Mourot |
| author_sort | Ophélie Uriot |
| collection | DOAJ |
| description | Despite promising health effects, the probiotic status of <i>Streptococcus thermophilus,</i> a lactic acid bacterium widely used in dairy industry, requires further documentation of its physiological status during human gastrointestinal passage. This study aimed to apply recombinant-based in vivo technology (R-IVET) to identify genes triggered in a <i>S. thermophilus</i> LMD-9 reference strain under simulated digestive conditions. First, the R-IVET chromosomal cassette and plasmid genomic library were designed to positively select activated genes. Second, recombinant clones were introduced into complementary models mimicking the human gut, the Netherlands Organization for Applied Scientific Research (TNO) gastrointestinal model imitating the human stomach and small intestine, the Caco-2 TC7 cell line as a model of intestinal epithelium, and anaerobic batch cultures of human feces as a colon model. All inserts of activated clones displayed a promoter activity that differed from one digestive condition to another. Our results also showed that <i>S. thermophilus</i> adapted its metabolism to stressful conditions found in the gastric and colonic competitive environment and modified its surface proteins during adhesion to Caco-2 TC7 cells. Activated genes were investigated in a collection of <i>S. thermophilus</i> strains showing various resistance levels to gastrointestinal stresses, a first stage in the identification of gut resistance markers and a key step in probiotic selection. |
| first_indexed | 2024-03-10T11:11:39Z |
| format | Article |
| id | doaj.art-cf0fa6ef97284214afdd240d23d51fb0 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T11:11:39Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-cf0fa6ef97284214afdd240d23d51fb02023-11-21T20:47:16ZengMDPI AGMicroorganisms2076-26072021-05-0196111310.3390/microorganisms9061113Identification of <i>Streptococcus thermophilus</i> Genes Specifically Expressed under Simulated Human Digestive Conditions Using R-IVET TechnologyOphélie Uriot0Mounira Kebouchi1Emilie Lorson2Wessam Galia3Sylvain Denis4Sandrine Chalancon5Zeeshan Hafeez6Emeline Roux7Magali Genay8Stéphanie Blanquet-Diot9Annie Dary-Mourot10EA 7488 Calbinotox Composés Alimentaires Biofonctionnalités & Risque Neurotoxique, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, FranceEA 7488 Calbinotox Composés Alimentaires Biofonctionnalités & Risque Neurotoxique, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, FranceEA 7488 Calbinotox Composés Alimentaires Biofonctionnalités & Risque Neurotoxique, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, FranceEA 7488 Calbinotox Composés Alimentaires Biofonctionnalités & Risque Neurotoxique, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, FranceUMR 454 MEDIS Microbiology, Digestive Environment and Health, Université Clermont Auvergne, INRAe, 63000 Clermont-Ferrand, FranceUMR 454 MEDIS Microbiology, Digestive Environment and Health, Université Clermont Auvergne, INRAe, 63000 Clermont-Ferrand, FranceEA 7488 Calbinotox Composés Alimentaires Biofonctionnalités & Risque Neurotoxique, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, FranceEA 7488 Calbinotox Composés Alimentaires Biofonctionnalités & Risque Neurotoxique, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, FranceEA 7488 Calbinotox Composés Alimentaires Biofonctionnalités & Risque Neurotoxique, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, FranceUMR 454 MEDIS Microbiology, Digestive Environment and Health, Université Clermont Auvergne, INRAe, 63000 Clermont-Ferrand, FranceEA 7488 Calbinotox Composés Alimentaires Biofonctionnalités & Risque Neurotoxique, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, FranceDespite promising health effects, the probiotic status of <i>Streptococcus thermophilus,</i> a lactic acid bacterium widely used in dairy industry, requires further documentation of its physiological status during human gastrointestinal passage. This study aimed to apply recombinant-based in vivo technology (R-IVET) to identify genes triggered in a <i>S. thermophilus</i> LMD-9 reference strain under simulated digestive conditions. First, the R-IVET chromosomal cassette and plasmid genomic library were designed to positively select activated genes. Second, recombinant clones were introduced into complementary models mimicking the human gut, the Netherlands Organization for Applied Scientific Research (TNO) gastrointestinal model imitating the human stomach and small intestine, the Caco-2 TC7 cell line as a model of intestinal epithelium, and anaerobic batch cultures of human feces as a colon model. All inserts of activated clones displayed a promoter activity that differed from one digestive condition to another. Our results also showed that <i>S. thermophilus</i> adapted its metabolism to stressful conditions found in the gastric and colonic competitive environment and modified its surface proteins during adhesion to Caco-2 TC7 cells. Activated genes were investigated in a collection of <i>S. thermophilus</i> strains showing various resistance levels to gastrointestinal stresses, a first stage in the identification of gut resistance markers and a key step in probiotic selection.https://www.mdpi.com/2076-2607/9/6/1113<i>S. thermophilus</i>R-IVETTIM-1 systemintestinal microbiotaadhesion |
| spellingShingle | Ophélie Uriot Mounira Kebouchi Emilie Lorson Wessam Galia Sylvain Denis Sandrine Chalancon Zeeshan Hafeez Emeline Roux Magali Genay Stéphanie Blanquet-Diot Annie Dary-Mourot Identification of <i>Streptococcus thermophilus</i> Genes Specifically Expressed under Simulated Human Digestive Conditions Using R-IVET Technology Microorganisms <i>S. thermophilus</i> R-IVET TIM-1 system intestinal microbiota adhesion |
| title | Identification of <i>Streptococcus thermophilus</i> Genes Specifically Expressed under Simulated Human Digestive Conditions Using R-IVET Technology |
| title_full | Identification of <i>Streptococcus thermophilus</i> Genes Specifically Expressed under Simulated Human Digestive Conditions Using R-IVET Technology |
| title_fullStr | Identification of <i>Streptococcus thermophilus</i> Genes Specifically Expressed under Simulated Human Digestive Conditions Using R-IVET Technology |
| title_full_unstemmed | Identification of <i>Streptococcus thermophilus</i> Genes Specifically Expressed under Simulated Human Digestive Conditions Using R-IVET Technology |
| title_short | Identification of <i>Streptococcus thermophilus</i> Genes Specifically Expressed under Simulated Human Digestive Conditions Using R-IVET Technology |
| title_sort | identification of i streptococcus thermophilus i genes specifically expressed under simulated human digestive conditions using r ivet technology |
| topic | <i>S. thermophilus</i> R-IVET TIM-1 system intestinal microbiota adhesion |
| url | https://www.mdpi.com/2076-2607/9/6/1113 |
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