<b>Fungal production of the anti-leukemic enzyme L-asparaginase: from screening to medium development
The treatment of acute lymphoblastic leukemia is challenging due to side effects, efficacy of the available drugs, and costs. Utilization of L-asparaginase as a therapeutic agent is essential to increase survival of patients. However, costs are elevated and the bacterial forms of the enzyme cause re...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2016-12-01
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| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | https://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/32993 |
| _version_ | 1811326284776603648 |
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| author | Aline Bacelar Gonçalves Ana Carolina Ferreira Maia Jorge Andres Rueda Ana Paula de Figueiredo Conte Vanzela |
| author_facet | Aline Bacelar Gonçalves Ana Carolina Ferreira Maia Jorge Andres Rueda Ana Paula de Figueiredo Conte Vanzela |
| author_sort | Aline Bacelar Gonçalves |
| collection | DOAJ |
| description | The treatment of acute lymphoblastic leukemia is challenging due to side effects, efficacy of the available drugs, and costs. Utilization of L-asparaginase as a therapeutic agent is essential to increase survival of patients. However, costs are elevated and the bacterial forms of the enzyme cause reactions that result in its inhibition by the immune system. Therapeutics alternatives may be searched among eukaryote producers, like fungi. Twelve strains of filamentous fungi were evaluated regarding expression of L-asparaginase activity. The profile of nitrogen assimilation and radial growth were determined for strains which showed higher production ratios. Three media were formulated after selection of the carbon source and carbon/nitrogen ratio that better induced L-asparaginase expression by Penicillium sp. and Fusarium sp. The enzyme activity produced in liquid media reached 8.3 U min.-1 mL-1 (Penicillium sp. T6.2) and 11.4 U min.-1 mL-1 (Fusarium sp.) after 72 hours of cultivation in Bacelar-1 medium. These data show that good producers can be found among fungi, and adjustment of productive processes may offer an alternative to implement eukaryote L-asparaginase production.
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| first_indexed | 2024-04-13T14:47:18Z |
| format | Article |
| id | doaj.art-cf11151e3abe4cbb98b4ba9b970db38c |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-04-13T14:47:18Z |
| publishDate | 2016-12-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-cf11151e3abe4cbb98b4ba9b970db38c2022-12-22T02:42:42ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2016-12-0138410.4025/actascibiolsci.v38i4.3299314837<b>Fungal production of the anti-leukemic enzyme L-asparaginase: from screening to medium developmentAline Bacelar Gonçalves0Ana Carolina Ferreira Maia1Jorge Andres Rueda2Ana Paula de Figueiredo Conte Vanzela3Universidade Federal dos Vales do Jequitinhonha e MucuriUniversidade Federal dos Vales do Jequitinhonha e MucuriUniversidade Federal dos Vales do Jequitinhonha e MucuriUniversidade Federal dos Vales do Jequitinhonha e MucuriThe treatment of acute lymphoblastic leukemia is challenging due to side effects, efficacy of the available drugs, and costs. Utilization of L-asparaginase as a therapeutic agent is essential to increase survival of patients. However, costs are elevated and the bacterial forms of the enzyme cause reactions that result in its inhibition by the immune system. Therapeutics alternatives may be searched among eukaryote producers, like fungi. Twelve strains of filamentous fungi were evaluated regarding expression of L-asparaginase activity. The profile of nitrogen assimilation and radial growth were determined for strains which showed higher production ratios. Three media were formulated after selection of the carbon source and carbon/nitrogen ratio that better induced L-asparaginase expression by Penicillium sp. and Fusarium sp. The enzyme activity produced in liquid media reached 8.3 U min.-1 mL-1 (Penicillium sp. T6.2) and 11.4 U min.-1 mL-1 (Fusarium sp.) after 72 hours of cultivation in Bacelar-1 medium. These data show that good producers can be found among fungi, and adjustment of productive processes may offer an alternative to implement eukaryote L-asparaginase production. https://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/32993therapeutic enzymesfilamentous fungiacute lymphoblastic leukemiaPenicillium sp.Fusarium sp. |
| spellingShingle | Aline Bacelar Gonçalves Ana Carolina Ferreira Maia Jorge Andres Rueda Ana Paula de Figueiredo Conte Vanzela <b>Fungal production of the anti-leukemic enzyme L-asparaginase: from screening to medium development Acta Scientiarum: Biological Sciences therapeutic enzymes filamentous fungi acute lymphoblastic leukemia Penicillium sp. Fusarium sp. |
| title | <b>Fungal production of the anti-leukemic enzyme L-asparaginase: from screening to medium development |
| title_full | <b>Fungal production of the anti-leukemic enzyme L-asparaginase: from screening to medium development |
| title_fullStr | <b>Fungal production of the anti-leukemic enzyme L-asparaginase: from screening to medium development |
| title_full_unstemmed | <b>Fungal production of the anti-leukemic enzyme L-asparaginase: from screening to medium development |
| title_short | <b>Fungal production of the anti-leukemic enzyme L-asparaginase: from screening to medium development |
| title_sort | b fungal production of the anti leukemic enzyme l asparaginase from screening to medium development |
| topic | therapeutic enzymes filamentous fungi acute lymphoblastic leukemia Penicillium sp. Fusarium sp. |
| url | https://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/32993 |
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