A three-dimensional matrix system containing melatonin and neural stem cells repairs damage from traumatic brain injury in rats
[INLINE:1] Brain lesions can cause neural stem cells to activate, proliferate, differentiate, and migrate to the injured area. However, after traumatic brain injury, brain tissue defects and microenvironment changes greatly affect the survival and growth of neural stem cells; the resulting reduction...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=11;spage=2512;epage=2517;aulast=Fang |
| _version_ | 1811244905220014080 |
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| author | Xuan-Yu Fang Da-Wei Zhao Chao Zhang Hong-Fei Ge Xu-Yang Zhang Feng-Chun Zhao Yi-Bin Jiang Hua Feng Rong Hu |
| author_facet | Xuan-Yu Fang Da-Wei Zhao Chao Zhang Hong-Fei Ge Xu-Yang Zhang Feng-Chun Zhao Yi-Bin Jiang Hua Feng Rong Hu |
| author_sort | Xuan-Yu Fang |
| collection | DOAJ |
| description | [INLINE:1]
Brain lesions can cause neural stem cells to activate, proliferate, differentiate, and migrate to the injured area. However, after traumatic brain injury, brain tissue defects and microenvironment changes greatly affect the survival and growth of neural stem cells; the resulting reduction in the number of neural stem cells impedes effective repair of the injured area. Melatonin can promote the survival, proliferation, and differentiation of neural stem cells under adverse conditions such as oxidative stress or hypoxia that can occur after traumatic brain injury. Therefore, we investigated the therapeutic effects of melatonin combined with neural stem cells on traumatic brain injury in rats. First, in vitro studies confirmed that melatonin promoted the survival of neural stem cells deprived of oxygen and glucose. Then, we established a three-dimensional Matrigel-based transplantation system containing melatonin and neural stem cells and then used it to treat traumatic brain injury in rats. We found that treatment with the Matrigel system containing melatonin and neural stem cells decreased brain lesion volume, increased the number of surviving neurons, and improved recovery of neurological function compared with treatment with Matrigel alone, neural stem cells alone, Matrigel and neural stem cells combined, and Matrigel and melatonin combined. Our findings suggest that the three-dimensional Matrigel-based transplantation system containing melatonin and neural stem cells is a potential treatment for traumatic brain injury. |
| first_indexed | 2024-04-12T14:32:47Z |
| format | Article |
| id | doaj.art-cf12d6020acd465fac9fe5e6ea1f249e |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-04-12T14:32:47Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-cf12d6020acd465fac9fe5e6ea1f249e2022-12-22T03:29:13ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742022-01-0117112512251710.4103/1673-5374.339001A three-dimensional matrix system containing melatonin and neural stem cells repairs damage from traumatic brain injury in ratsXuan-Yu FangDa-Wei ZhaoChao ZhangHong-Fei GeXu-Yang ZhangFeng-Chun ZhaoYi-Bin JiangHua FengRong Hu[INLINE:1] Brain lesions can cause neural stem cells to activate, proliferate, differentiate, and migrate to the injured area. However, after traumatic brain injury, brain tissue defects and microenvironment changes greatly affect the survival and growth of neural stem cells; the resulting reduction in the number of neural stem cells impedes effective repair of the injured area. Melatonin can promote the survival, proliferation, and differentiation of neural stem cells under adverse conditions such as oxidative stress or hypoxia that can occur after traumatic brain injury. Therefore, we investigated the therapeutic effects of melatonin combined with neural stem cells on traumatic brain injury in rats. First, in vitro studies confirmed that melatonin promoted the survival of neural stem cells deprived of oxygen and glucose. Then, we established a three-dimensional Matrigel-based transplantation system containing melatonin and neural stem cells and then used it to treat traumatic brain injury in rats. We found that treatment with the Matrigel system containing melatonin and neural stem cells decreased brain lesion volume, increased the number of surviving neurons, and improved recovery of neurological function compared with treatment with Matrigel alone, neural stem cells alone, Matrigel and neural stem cells combined, and Matrigel and melatonin combined. Our findings suggest that the three-dimensional Matrigel-based transplantation system containing melatonin and neural stem cells is a potential treatment for traumatic brain injury.http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=11;spage=2512;epage=2517;aulast=Fangcell therapy; magnetic resonance imaging; matrigel; melatonin; neural stem cells; neurological function recovery; three-dimensional transplantation; traumatic brain injury |
| spellingShingle | Xuan-Yu Fang Da-Wei Zhao Chao Zhang Hong-Fei Ge Xu-Yang Zhang Feng-Chun Zhao Yi-Bin Jiang Hua Feng Rong Hu A three-dimensional matrix system containing melatonin and neural stem cells repairs damage from traumatic brain injury in rats Neural Regeneration Research cell therapy; magnetic resonance imaging; matrigel; melatonin; neural stem cells; neurological function recovery; three-dimensional transplantation; traumatic brain injury |
| title | A three-dimensional matrix system containing melatonin and neural stem cells repairs damage from traumatic brain injury in rats |
| title_full | A three-dimensional matrix system containing melatonin and neural stem cells repairs damage from traumatic brain injury in rats |
| title_fullStr | A three-dimensional matrix system containing melatonin and neural stem cells repairs damage from traumatic brain injury in rats |
| title_full_unstemmed | A three-dimensional matrix system containing melatonin and neural stem cells repairs damage from traumatic brain injury in rats |
| title_short | A three-dimensional matrix system containing melatonin and neural stem cells repairs damage from traumatic brain injury in rats |
| title_sort | three dimensional matrix system containing melatonin and neural stem cells repairs damage from traumatic brain injury in rats |
| topic | cell therapy; magnetic resonance imaging; matrigel; melatonin; neural stem cells; neurological function recovery; three-dimensional transplantation; traumatic brain injury |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=11;spage=2512;epage=2517;aulast=Fang |
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