Molecular Detection of <em>Babesia gibsoni</em> in Cats in China
As there are few studies of <i>Babesia</i> spp. infection in cats in China, or anywhere in the world, the aim of this study was to explore the epidemic features of babesiosis in pet cats in China. In total, 429 blood samples were randomly collected in four different geographical regions....
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/22/3066 |
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| author | Fangyuan Yin Daoe Mu Zhuojia Tian Dong Li Xiting Ma Jinming Wang Guiquan Guan Hong Yin Facai Li |
| author_facet | Fangyuan Yin Daoe Mu Zhuojia Tian Dong Li Xiting Ma Jinming Wang Guiquan Guan Hong Yin Facai Li |
| author_sort | Fangyuan Yin |
| collection | DOAJ |
| description | As there are few studies of <i>Babesia</i> spp. infection in cats in China, or anywhere in the world, the aim of this study was to explore the epidemic features of babesiosis in pet cats in China. In total, 429 blood samples were randomly collected in four different geographical regions. The 18S rRNA gene fragment of <i>Babesia</i> spp. was amplified by nest polymerase chain reaction (PCR), and haplotype and phylogenetic analysis of <i>Babesia</i> were performed to analyze the relationship of this protozoa. The total positive rate of infection was 2.8%. BLAST analysis indicated that <i>Babesia gibsoni</i> was detected in 12 cats. Among these, 4.3%, 3.1%, 0.8% and 2.0% were from Chongqing, Fujian, Hubei and Shandong, respectively. Haplotype and phylogenetic analysis showed that there were nine haplotypes and no obvious genetic variation among <i>B. gibsoni</i> populations. These findings will be helpful for understanding the epidemiology of <i>Babesia</i> spp. in China, and provide a foundation for developing effective preventative strategies. |
| first_indexed | 2024-03-09T18:32:38Z |
| format | Article |
| id | doaj.art-cf165a2f2d60453080affeaec9329699 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T18:32:38Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-cf165a2f2d60453080affeaec93296992023-11-24T07:27:45ZengMDPI AGAnimals2076-26152022-11-011222306610.3390/ani12223066Molecular Detection of <em>Babesia gibsoni</em> in Cats in ChinaFangyuan Yin0Daoe Mu1Zhuojia Tian2Dong Li3Xiting Ma4Jinming Wang5Guiquan Guan6Hong Yin7Facai Li8College of Veterinary Medicine, Southwest University, Chongqing 400715, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 400715, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 400715, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 400715, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 400715, ChinaState Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730046, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 400715, ChinaAs there are few studies of <i>Babesia</i> spp. infection in cats in China, or anywhere in the world, the aim of this study was to explore the epidemic features of babesiosis in pet cats in China. In total, 429 blood samples were randomly collected in four different geographical regions. The 18S rRNA gene fragment of <i>Babesia</i> spp. was amplified by nest polymerase chain reaction (PCR), and haplotype and phylogenetic analysis of <i>Babesia</i> were performed to analyze the relationship of this protozoa. The total positive rate of infection was 2.8%. BLAST analysis indicated that <i>Babesia gibsoni</i> was detected in 12 cats. Among these, 4.3%, 3.1%, 0.8% and 2.0% were from Chongqing, Fujian, Hubei and Shandong, respectively. Haplotype and phylogenetic analysis showed that there were nine haplotypes and no obvious genetic variation among <i>B. gibsoni</i> populations. These findings will be helpful for understanding the epidemiology of <i>Babesia</i> spp. in China, and provide a foundation for developing effective preventative strategies.https://www.mdpi.com/2076-2615/12/22/3066<i>Babesia gibsoni</i><i>Babesia</i> spp. in felinepet cats18S rRNAChina |
| spellingShingle | Fangyuan Yin Daoe Mu Zhuojia Tian Dong Li Xiting Ma Jinming Wang Guiquan Guan Hong Yin Facai Li Molecular Detection of <em>Babesia gibsoni</em> in Cats in China Animals <i>Babesia gibsoni</i> <i>Babesia</i> spp. in feline pet cats 18S rRNA China |
| title | Molecular Detection of <em>Babesia gibsoni</em> in Cats in China |
| title_full | Molecular Detection of <em>Babesia gibsoni</em> in Cats in China |
| title_fullStr | Molecular Detection of <em>Babesia gibsoni</em> in Cats in China |
| title_full_unstemmed | Molecular Detection of <em>Babesia gibsoni</em> in Cats in China |
| title_short | Molecular Detection of <em>Babesia gibsoni</em> in Cats in China |
| title_sort | molecular detection of em babesia gibsoni em in cats in china |
| topic | <i>Babesia gibsoni</i> <i>Babesia</i> spp. in feline pet cats 18S rRNA China |
| url | https://www.mdpi.com/2076-2615/12/22/3066 |
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