Detection of botulinum neurotoxin serotypes c and d, and their effects on expressions of snap-25 and synaptobrevin in ruminants: an immunohistochemical study
In humans and animals, botulism is a disease characterized by generalized and progressive paralysis caused by Clostridium botulinum neurotoxins (BoNT). BoNTs, defined in seven diff erent antigenic types (A to G), proteolyze SNAREs (synaptosomal-associated protein/SNAP-25 and synaptobrevin) responsib...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2021-02-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2783 |
| _version_ | 1797800734731796480 |
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| author | Mehmet Burak ATEŞ Funda TERZİ Mustafa Kemal ÇİFTÇİ Mustafa ORTATATLI Zeynep ÇELİK |
| author_facet | Mehmet Burak ATEŞ Funda TERZİ Mustafa Kemal ÇİFTÇİ Mustafa ORTATATLI Zeynep ÇELİK |
| author_sort | Mehmet Burak ATEŞ |
| collection | DOAJ |
| description | In humans and animals, botulism is a disease characterized by generalized and progressive paralysis caused by Clostridium botulinum neurotoxins
(BoNT). BoNTs, defined in seven diff erent antigenic types (A to G), proteolyze SNAREs (synaptosomal-associated protein/SNAP-25 and synaptobrevin)
responsible for acetylcholine release in peripheral cholinergic neurons, and thus cause fl accid paralysis and death. Currently, mouse experiments
are considered the reference method for definitive diagnosis. However, new diagnostic methods that are fast and accurate and would not raise
ethical issues need to be developed. Therefore, using antibodies specific to the toxoid forms of BoNTs, the presence of BoNT-C and/or BoNT-D was
investigated by immunohistochemical method (IHC) in the study. The tissues of thirty ruminants (twenty cattle, seven sheep, three goats), which had
the clinical and pathological findings of botulism and a herd history of the disease, were used as material. BoNTs were detected with IHC in sixteen
of the thirty ruminants as three BoNT-C, eleven BoNT-D, and two BoNT C+D. In the mouse experiments, BoNT was isolated in only three cases (two
BoNT-D, one BoNT-C). Additionally, being responsible for the clinical findings of botulism, the interaction of BoNTs with SNAP-25 and synaptobrevin
was investigated using IHC. It was determined that BoNT-C specifically reduces the expression of SNAP-25, and BoNT-D reduces the expression of
synaptobrevin and partially SNAP-25. It was concluded that additional studies may be valuable to investigate the use of IHC in the diagnosis of
botulism. |
| first_indexed | 2024-03-13T04:39:43Z |
| format | Article |
| id | doaj.art-cf1949ca1fd14901879b261f99245fc1 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:39:43Z |
| publishDate | 2021-02-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-cf1949ca1fd14901879b261f99245fc12023-06-19T06:30:05ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512021-02-0127219120110.9775/kvfd.2020.250602783Detection of botulinum neurotoxin serotypes c and d, and their effects on expressions of snap-25 and synaptobrevin in ruminants: an immunohistochemical studyMehmet Burak ATEŞ0Funda TERZİ1Mustafa Kemal ÇİFTÇİ2Mustafa ORTATATLI3Zeynep ÇELİK4Selçuk University, Faculty of Veterinary Medicine, Department of Pathology, TR-42130 Konya - TURKEYKastamonu University, Faculty of Veterinary Medicine, Department of Pathology, TR-37200, Kastamonu - TURKEYSelçuk University, Faculty of Veterinary Medicine, Department of Pathology, TR-42130 Konya - TURKEYSelçuk University, Faculty of Veterinary Medicine, Department of Pathology, TR-42130 Konya - TURKEYSelçuk University, Faculty of Veterinary Medicine, Department of Pathology, TR-42130 Konya - TURKEYIn humans and animals, botulism is a disease characterized by generalized and progressive paralysis caused by Clostridium botulinum neurotoxins (BoNT). BoNTs, defined in seven diff erent antigenic types (A to G), proteolyze SNAREs (synaptosomal-associated protein/SNAP-25 and synaptobrevin) responsible for acetylcholine release in peripheral cholinergic neurons, and thus cause fl accid paralysis and death. Currently, mouse experiments are considered the reference method for definitive diagnosis. However, new diagnostic methods that are fast and accurate and would not raise ethical issues need to be developed. Therefore, using antibodies specific to the toxoid forms of BoNTs, the presence of BoNT-C and/or BoNT-D was investigated by immunohistochemical method (IHC) in the study. The tissues of thirty ruminants (twenty cattle, seven sheep, three goats), which had the clinical and pathological findings of botulism and a herd history of the disease, were used as material. BoNTs were detected with IHC in sixteen of the thirty ruminants as three BoNT-C, eleven BoNT-D, and two BoNT C+D. In the mouse experiments, BoNT was isolated in only three cases (two BoNT-D, one BoNT-C). Additionally, being responsible for the clinical findings of botulism, the interaction of BoNTs with SNAP-25 and synaptobrevin was investigated using IHC. It was determined that BoNT-C specifically reduces the expression of SNAP-25, and BoNT-D reduces the expression of synaptobrevin and partially SNAP-25. It was concluded that additional studies may be valuable to investigate the use of IHC in the diagnosis of botulism.https://vetdergikafkas.org/pdf.php?id=2783bont-cbont-dsnap-25synaptobrevinimmunohistochemistry |
| spellingShingle | Mehmet Burak ATEŞ Funda TERZİ Mustafa Kemal ÇİFTÇİ Mustafa ORTATATLI Zeynep ÇELİK Detection of botulinum neurotoxin serotypes c and d, and their effects on expressions of snap-25 and synaptobrevin in ruminants: an immunohistochemical study Kafkas Universitesi Veteriner Fakültesi Dergisi bont-c bont-d snap-25 synaptobrevin immunohistochemistry |
| title | Detection of botulinum neurotoxin serotypes c and d, and their effects on expressions of snap-25 and synaptobrevin in ruminants: an immunohistochemical study |
| title_full | Detection of botulinum neurotoxin serotypes c and d, and their effects on expressions of snap-25 and synaptobrevin in ruminants: an immunohistochemical study |
| title_fullStr | Detection of botulinum neurotoxin serotypes c and d, and their effects on expressions of snap-25 and synaptobrevin in ruminants: an immunohistochemical study |
| title_full_unstemmed | Detection of botulinum neurotoxin serotypes c and d, and their effects on expressions of snap-25 and synaptobrevin in ruminants: an immunohistochemical study |
| title_short | Detection of botulinum neurotoxin serotypes c and d, and their effects on expressions of snap-25 and synaptobrevin in ruminants: an immunohistochemical study |
| title_sort | detection of botulinum neurotoxin serotypes c and d and their effects on expressions of snap 25 and synaptobrevin in ruminants an immunohistochemical study |
| topic | bont-c bont-d snap-25 synaptobrevin immunohistochemistry |
| url | https://vetdergikafkas.org/pdf.php?id=2783 |
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