Characteristics of <i>Streptococcus pneumoniae</i> carriage in the pediatric population
Objective: to investigate the regional peculiarities of Streptococcus pneumoniae carriage in the pediatric population and characterize the dominant serotypes of the pathogen. Materials and methods. The clinical study group consisted of 509 healthy children attending preschool institutions. Examin...
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2024-03-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
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| Online Access: | https://microbiol.crie.ru/jour/article/viewFile/18556/1406 |
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| author | Guzel S. Isaeva Albina Z. Zaripova Lira T. Bayazitova Ralina M. Khusainova Tatiana A. Chazova Olga F. Tyupkina Ekaterina V. Nikitina Irina A. Tsvetkova |
| author_facet | Guzel S. Isaeva Albina Z. Zaripova Lira T. Bayazitova Ralina M. Khusainova Tatiana A. Chazova Olga F. Tyupkina Ekaterina V. Nikitina Irina A. Tsvetkova |
| author_sort | Guzel S. Isaeva |
| collection | DOAJ |
| description | Objective: to investigate the regional peculiarities of Streptococcus pneumoniae carriage in the pediatric population and characterize the dominant serotypes of the pathogen.
Materials and methods. The clinical study group consisted of 509 healthy children attending preschool institutions. Examination of nasopharyngeal samples for the detection of S. pneumonae was carried out by classical bacteriological and molecular biological methods. The serotype was determined by real-time PCR. Genome-wide sequencing of the serogroups 15 and 11 isolates and bioinformatic analysis were performed.
Results. The S. pneumoniae bacterial carriers in the group of healthy children was detected in 207 children (40.7%), while the frequency of detection of S. pneumoniae in urban children living in Kazan was significantly higher than in children living in rural area and amounted to 53.4 and 31.1%, respectively (p 0.05). Among children vaccinated with the 13-valent pneumococcal conjugate vaccine (PCV-13), S. pneumoniae carriers were not detected in 57.5% of cases. There were no significant differences in the degree of nasopharyngeal contamination depending on the vaccination status. Analysis of the serotype composition indicates the predominance of vaccine serotypes (57.7%), while the share of serotypes included in the PСV-13 vaccine accounts for only 24.7%, the share of non-vaccine serotypes was 32.1%, untyped — 10.2%. In unvaccinated children, vaccine serotypes that are part of the PCV-13 and 23-valent polysaccharide pneumococcal vaccine prevailed (PPSV-23): 6ABCD (21%), 11 AD (15%), 14 (13%). In vaccinated children, serotypes not included in the active vaccines dominated: 15AF (17.4%), 23A (19.2%), as well as 11AD (19.6%) (11А is included in PPSV-23). The 27 Kz isolate (serotype 15C) belonged to one of the most common sequence types ST1025. The 105_Kz isolate (serotype 11D) belonged to another common sequence type ST 62.
Conclusion. In order to improve epidemiological surveillance of pneumococcal infection, it is necessary to introduce the monitoring of circulating clonal complexes of dominant S. pneumoniae serogroups and analyze the genetic determinants of antibiotic resistance and virulence depending on the sequence type. |
| first_indexed | 2024-04-24T22:33:04Z |
| format | Article |
| id | doaj.art-cf1ef4cfcfc74e4d81cf0a000097c34c |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-04-24T22:33:04Z |
| publishDate | 2024-03-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-cf1ef4cfcfc74e4d81cf0a000097c34c2024-03-19T14:53:29ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132024-03-011011899910.36233/0372-9311-4452734Characteristics of <i>Streptococcus pneumoniae</i> carriage in the pediatric populationGuzel S. Isaeva0https://orcid.org/0000-0002-1462-8734Albina Z. Zaripova1https://orcid.org/0000-0001-6790-0538Lira T. Bayazitova2https://orcid.org/0000-0002-2142-7682Ralina M. Khusainova3https://orcid.org/0000-0002-4733-3959Tatiana A. Chazova4https://orcid.org/0000-0002-2013-4239Olga F. Tyupkina5https://orcid.org/0000-0001-8180-1165Ekaterina V. Nikitina6https://orcid.org/0000-0002-9737-9496Irina A. Tsvetkova7https://orcid.org/0000-0002-0170-6975Kazan Scientific Research Institute of Epidemiology and MicrobiologyKazan State Medical UniversityKazan Scientific Research Institute of Epidemiology and MicrobiologyKazan Scientific Research Institute of Epidemiology and MicrobiologyKazan Scientific Research Institute of Epidemiology and MicrobiologyKazan Scientific Research Institute of Epidemiology and MicrobiologyPediatric Research and Clinical Center for Infectious DiseasesPediatric Research and Clinical Center for Infectious DiseasesObjective: to investigate the regional peculiarities of Streptococcus pneumoniae carriage in the pediatric population and characterize the dominant serotypes of the pathogen. Materials and methods. The clinical study group consisted of 509 healthy children attending preschool institutions. Examination of nasopharyngeal samples for the detection of S. pneumonae was carried out by classical bacteriological and molecular biological methods. The serotype was determined by real-time PCR. Genome-wide sequencing of the serogroups 15 and 11 isolates and bioinformatic analysis were performed. Results. The S. pneumoniae bacterial carriers in the group of healthy children was detected in 207 children (40.7%), while the frequency of detection of S. pneumoniae in urban children living in Kazan was significantly higher than in children living in rural area and amounted to 53.4 and 31.1%, respectively (p 0.05). Among children vaccinated with the 13-valent pneumococcal conjugate vaccine (PCV-13), S. pneumoniae carriers were not detected in 57.5% of cases. There were no significant differences in the degree of nasopharyngeal contamination depending on the vaccination status. Analysis of the serotype composition indicates the predominance of vaccine serotypes (57.7%), while the share of serotypes included in the PСV-13 vaccine accounts for only 24.7%, the share of non-vaccine serotypes was 32.1%, untyped — 10.2%. In unvaccinated children, vaccine serotypes that are part of the PCV-13 and 23-valent polysaccharide pneumococcal vaccine prevailed (PPSV-23): 6ABCD (21%), 11 AD (15%), 14 (13%). In vaccinated children, serotypes not included in the active vaccines dominated: 15AF (17.4%), 23A (19.2%), as well as 11AD (19.6%) (11А is included in PPSV-23). The 27 Kz isolate (serotype 15C) belonged to one of the most common sequence types ST1025. The 105_Kz isolate (serotype 11D) belonged to another common sequence type ST 62. Conclusion. In order to improve epidemiological surveillance of pneumococcal infection, it is necessary to introduce the monitoring of circulating clonal complexes of dominant S. pneumoniae serogroups and analyze the genetic determinants of antibiotic resistance and virulence depending on the sequence type.https://microbiol.crie.ru/jour/article/viewFile/18556/1406streptococcus pneumoniaebacterial carrierserotypessequence types |
| spellingShingle | Guzel S. Isaeva Albina Z. Zaripova Lira T. Bayazitova Ralina M. Khusainova Tatiana A. Chazova Olga F. Tyupkina Ekaterina V. Nikitina Irina A. Tsvetkova Characteristics of <i>Streptococcus pneumoniae</i> carriage in the pediatric population Журнал микробиологии, эпидемиологии и иммунобиологии streptococcus pneumoniae bacterial carrier serotypes sequence types |
| title | Characteristics of <i>Streptococcus pneumoniae</i> carriage in the pediatric population |
| title_full | Characteristics of <i>Streptococcus pneumoniae</i> carriage in the pediatric population |
| title_fullStr | Characteristics of <i>Streptococcus pneumoniae</i> carriage in the pediatric population |
| title_full_unstemmed | Characteristics of <i>Streptococcus pneumoniae</i> carriage in the pediatric population |
| title_short | Characteristics of <i>Streptococcus pneumoniae</i> carriage in the pediatric population |
| title_sort | characteristics of i streptococcus pneumoniae i carriage in the pediatric population |
| topic | streptococcus pneumoniae bacterial carrier serotypes sequence types |
| url | https://microbiol.crie.ru/jour/article/viewFile/18556/1406 |
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