Using Genetic Code Expansion for Protein Biochemical Studies
Protein identification has gone beyond simply using protein/peptide tags and labeling canonical amino acids. Genetic code expansion has allowed residue- or site-specific incorporation of non-canonical amino acids into proteins. By taking advantage of the unique properties of non-canonical amino acid...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-10-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2020.598577/full |
| _version_ | 1818342036889141248 |
|---|---|
| author | Christina Z. Chung Kazuaki Amikura Dieter Söll Dieter Söll |
| author_facet | Christina Z. Chung Kazuaki Amikura Dieter Söll Dieter Söll |
| author_sort | Christina Z. Chung |
| collection | DOAJ |
| description | Protein identification has gone beyond simply using protein/peptide tags and labeling canonical amino acids. Genetic code expansion has allowed residue- or site-specific incorporation of non-canonical amino acids into proteins. By taking advantage of the unique properties of non-canonical amino acids, we can identify spatiotemporal-specific protein states within living cells. Insertion of more than one non-canonical amino acid allows for selective labeling that can aid in the identification of weak or transient protein–protein interactions. This review will discuss recent studies applying genetic code expansion for protein labeling and identifying protein–protein interactions and offer considerations for future work in expanding genetic code expansion methods. |
| first_indexed | 2024-12-13T16:08:19Z |
| format | Article |
| id | doaj.art-cf1f2488a5724d46a38d70da3fc2375e |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-13T16:08:19Z |
| publishDate | 2020-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-cf1f2488a5724d46a38d70da3fc2375e2022-12-21T23:38:59ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-10-01810.3389/fbioe.2020.598577598577Using Genetic Code Expansion for Protein Biochemical StudiesChristina Z. Chung0Kazuaki Amikura1Dieter Söll2Dieter Söll3Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, United StatesDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, United StatesDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, United StatesDepartment of Chemistry, Yale University, New Haven, CT, United StatesProtein identification has gone beyond simply using protein/peptide tags and labeling canonical amino acids. Genetic code expansion has allowed residue- or site-specific incorporation of non-canonical amino acids into proteins. By taking advantage of the unique properties of non-canonical amino acids, we can identify spatiotemporal-specific protein states within living cells. Insertion of more than one non-canonical amino acid allows for selective labeling that can aid in the identification of weak or transient protein–protein interactions. This review will discuss recent studies applying genetic code expansion for protein labeling and identifying protein–protein interactions and offer considerations for future work in expanding genetic code expansion methods.https://www.frontiersin.org/article/10.3389/fbioe.2020.598577/fullgenetic code expansionnon-canonical amino acidsprotein labelingprotein purificationprotein–protein interactions |
| spellingShingle | Christina Z. Chung Kazuaki Amikura Dieter Söll Dieter Söll Using Genetic Code Expansion for Protein Biochemical Studies Frontiers in Bioengineering and Biotechnology genetic code expansion non-canonical amino acids protein labeling protein purification protein–protein interactions |
| title | Using Genetic Code Expansion for Protein Biochemical Studies |
| title_full | Using Genetic Code Expansion for Protein Biochemical Studies |
| title_fullStr | Using Genetic Code Expansion for Protein Biochemical Studies |
| title_full_unstemmed | Using Genetic Code Expansion for Protein Biochemical Studies |
| title_short | Using Genetic Code Expansion for Protein Biochemical Studies |
| title_sort | using genetic code expansion for protein biochemical studies |
| topic | genetic code expansion non-canonical amino acids protein labeling protein purification protein–protein interactions |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2020.598577/full |
| work_keys_str_mv | AT christinazchung usinggeneticcodeexpansionforproteinbiochemicalstudies AT kazuakiamikura usinggeneticcodeexpansionforproteinbiochemicalstudies AT dietersoll usinggeneticcodeexpansionforproteinbiochemicalstudies AT dietersoll usinggeneticcodeexpansionforproteinbiochemicalstudies |