Comparison of Urea Determination in Biological Samples by EnFETs Based on pH and pNH4 Detection

In this paper urea biosensors based on detection of pH and ammonium ions are presented. As transducers pH-sensitive ion-selective field effect transistors (ISFETs) and chemically modified FETs (ChemFETs) snsitive to ammonium ions were used.Results concerning urea determination by the bisensors in bl...

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Main Authors: Wladyslaw Torbicz, Marek Dawgul, Dorota G. Bijanowska
Format: Article
Language:English
Published: MDPI AG 2003-06-01
Series:Sensors
Subjects:
Online Access:http://www.mdpi.com/1424-8220/3/6/160/
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author Wladyslaw Torbicz
Marek Dawgul
Dorota G. Bijanowska
author_facet Wladyslaw Torbicz
Marek Dawgul
Dorota G. Bijanowska
author_sort Wladyslaw Torbicz
collection DOAJ
description In this paper urea biosensors based on detection of pH and ammonium ions are presented. As transducers pH-sensitive ion-selective field effect transistors (ISFETs) and chemically modified FETs (ChemFETs) snsitive to ammonium ions were used.Results concerning urea determination by the bisensors in blood plasma and in dialysate show that the biosensors can be applied for urea monitoring in the effluent dialysate. However in the case of pNH4 based EnFETs a special pre-treatment (dilution with tris/HCl buffer) of the samples is necessary.
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spelling doaj.art-cf21861bc84340bcad583a1f0576625d2022-12-22T04:22:49ZengMDPI AGSensors1424-82202003-06-013616016510.3390/s30600160Comparison of Urea Determination in Biological Samples by EnFETs Based on pH and pNH4 DetectionWladyslaw TorbiczMarek DawgulDorota G. BijanowskaIn this paper urea biosensors based on detection of pH and ammonium ions are presented. As transducers pH-sensitive ion-selective field effect transistors (ISFETs) and chemically modified FETs (ChemFETs) snsitive to ammonium ions were used.Results concerning urea determination by the bisensors in blood plasma and in dialysate show that the biosensors can be applied for urea monitoring in the effluent dialysate. However in the case of pNH4 based EnFETs a special pre-treatment (dilution with tris/HCl buffer) of the samples is necessary.http://www.mdpi.com/1424-8220/3/6/160/Urea detectionbiosensorsEnFETenzyme immobilisation
spellingShingle Wladyslaw Torbicz
Marek Dawgul
Dorota G. Bijanowska
Comparison of Urea Determination in Biological Samples by EnFETs Based on pH and pNH4 Detection
Sensors
Urea detection
biosensors
EnFET
enzyme immobilisation
title Comparison of Urea Determination in Biological Samples by EnFETs Based on pH and pNH4 Detection
title_full Comparison of Urea Determination in Biological Samples by EnFETs Based on pH and pNH4 Detection
title_fullStr Comparison of Urea Determination in Biological Samples by EnFETs Based on pH and pNH4 Detection
title_full_unstemmed Comparison of Urea Determination in Biological Samples by EnFETs Based on pH and pNH4 Detection
title_short Comparison of Urea Determination in Biological Samples by EnFETs Based on pH and pNH4 Detection
title_sort comparison of urea determination in biological samples by enfets based on ph and pnh4 detection
topic Urea detection
biosensors
EnFET
enzyme immobilisation
url http://www.mdpi.com/1424-8220/3/6/160/
work_keys_str_mv AT wladyslawtorbicz comparisonofureadeterminationinbiologicalsamplesbyenfetsbasedonphandpnh4detection
AT marekdawgul comparisonofureadeterminationinbiologicalsamplesbyenfetsbasedonphandpnh4detection
AT dorotagbijanowska comparisonofureadeterminationinbiologicalsamplesbyenfetsbasedonphandpnh4detection