Evaluating Adverse Effects of Inhaled Nanoparticles by Realistic In Vitro Technology
The number of daily products containing nanoparticles (NP) is rapidly increasing. NP in powders, dispersions, or sprays are a yet unknown risk for incidental exposure, especially at workplaces during NP production and processing, and for consumers of any health status and age using NP containing spr...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2017-02-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2079-4991/7/2/49 |
| _version_ | 1818585153133346816 |
|---|---|
| author | Marianne Geiser Natalie Jeannet Martin Fierz Heinz Burtscher |
| author_facet | Marianne Geiser Natalie Jeannet Martin Fierz Heinz Burtscher |
| author_sort | Marianne Geiser |
| collection | DOAJ |
| description | The number of daily products containing nanoparticles (NP) is rapidly increasing. NP in powders, dispersions, or sprays are a yet unknown risk for incidental exposure, especially at workplaces during NP production and processing, and for consumers of any health status and age using NP containing sprays. We developed the nano aerosol chamber for in vitro toxicity (NACIVT), a portable instrument for realistic safety testing of inhaled NP in vitro and evaluated effects of silver (Ag) and carbon (C) NP—which belong to the most widely used nanomaterials—on normal and compromised airway epithelia. We review the development, physical performance, and suitability of NACIVT for short and long-term exposures with air-liquid interface (ALI) cell cultures in regard to the prerequisites of a realistic in vitro test system for inhalation toxicology and in comparison to other commercially available, well characterized systems. We also review doses applied to cell cultures in vitro and acknowledge that a single exposure to realistic doses of spark generated 20-nm Ag- or CNP results in small, similar cellular responses to both NP types and that cytokine release generally increased with increasing NP dose. |
| first_indexed | 2024-12-16T08:32:32Z |
| format | Article |
| id | doaj.art-cf24a746a06e42bb8bd7080382336325 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-16T08:32:32Z |
| publishDate | 2017-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-cf24a746a06e42bb8bd70803823363252022-12-21T22:37:51ZengMDPI AGNanomaterials2079-49912017-02-01724910.3390/nano7020049nano7020049Evaluating Adverse Effects of Inhaled Nanoparticles by Realistic In Vitro TechnologyMarianne Geiser0Natalie Jeannet1Martin Fierz2Heinz Burtscher3Institute of Anatomy, University of Bern, Baltzerstrasse 2, P.O. Box 922, 3012 Bern, SwitzerlandInstitute of Anatomy, University of Bern, Baltzerstrasse 2, P.O. Box 922, 3012 Bern, SwitzerlandInstitute of Aerosol and Sensor Technology, University of Applied Sciences and Arts Northwestern Switzerland, Klosterzelgstrasse 2, 5210 Windisch, SwitzerlandInstitute of Aerosol and Sensor Technology, University of Applied Sciences and Arts Northwestern Switzerland, Klosterzelgstrasse 2, 5210 Windisch, SwitzerlandThe number of daily products containing nanoparticles (NP) is rapidly increasing. NP in powders, dispersions, or sprays are a yet unknown risk for incidental exposure, especially at workplaces during NP production and processing, and for consumers of any health status and age using NP containing sprays. We developed the nano aerosol chamber for in vitro toxicity (NACIVT), a portable instrument for realistic safety testing of inhaled NP in vitro and evaluated effects of silver (Ag) and carbon (C) NP—which belong to the most widely used nanomaterials—on normal and compromised airway epithelia. We review the development, physical performance, and suitability of NACIVT for short and long-term exposures with air-liquid interface (ALI) cell cultures in regard to the prerequisites of a realistic in vitro test system for inhalation toxicology and in comparison to other commercially available, well characterized systems. We also review doses applied to cell cultures in vitro and acknowledge that a single exposure to realistic doses of spark generated 20-nm Ag- or CNP results in small, similar cellular responses to both NP types and that cytokine release generally increased with increasing NP dose.http://www.mdpi.com/2079-4991/7/2/493Raerosolair-liquid interfaceairway epitheliaelectrostatic depositionengineered nanoparticlesin vitroNACIVTtoxicologynanoparticles |
| spellingShingle | Marianne Geiser Natalie Jeannet Martin Fierz Heinz Burtscher Evaluating Adverse Effects of Inhaled Nanoparticles by Realistic In Vitro Technology Nanomaterials 3R aerosol air-liquid interface airway epithelia electrostatic deposition engineered nanoparticles in vitro NACIVT toxicology nanoparticles |
| title | Evaluating Adverse Effects of Inhaled Nanoparticles by Realistic In Vitro Technology |
| title_full | Evaluating Adverse Effects of Inhaled Nanoparticles by Realistic In Vitro Technology |
| title_fullStr | Evaluating Adverse Effects of Inhaled Nanoparticles by Realistic In Vitro Technology |
| title_full_unstemmed | Evaluating Adverse Effects of Inhaled Nanoparticles by Realistic In Vitro Technology |
| title_short | Evaluating Adverse Effects of Inhaled Nanoparticles by Realistic In Vitro Technology |
| title_sort | evaluating adverse effects of inhaled nanoparticles by realistic in vitro technology |
| topic | 3R aerosol air-liquid interface airway epithelia electrostatic deposition engineered nanoparticles in vitro NACIVT toxicology nanoparticles |
| url | http://www.mdpi.com/2079-4991/7/2/49 |
| work_keys_str_mv | AT mariannegeiser evaluatingadverseeffectsofinhalednanoparticlesbyrealisticinvitrotechnology AT nataliejeannet evaluatingadverseeffectsofinhalednanoparticlesbyrealisticinvitrotechnology AT martinfierz evaluatingadverseeffectsofinhalednanoparticlesbyrealisticinvitrotechnology AT heinzburtscher evaluatingadverseeffectsofinhalednanoparticlesbyrealisticinvitrotechnology |