Characterization of human serum albumin's interactions with safranal and crocin using multi-spectroscopic and molecular docking techniques
Interaction mechanisms of human serum albumin (HSA) with safranal and crocin were studied using UV–Vis absorption, fluorescence quenching and circular dichroism (CD) spectroscopies as well as molecular docking techniques. Changes in absorbance and fluorescence of HSA upon interactions with both comp...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-12-01
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| Series: | Biochemistry and Biophysics Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580819300822 |
| _version_ | 1818137909585248256 |
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| author | Alaa A. Salem Mohamed Lotfy Amr Amin Mohammad A. Ghattas |
| author_facet | Alaa A. Salem Mohamed Lotfy Amr Amin Mohammad A. Ghattas |
| author_sort | Alaa A. Salem |
| collection | DOAJ |
| description | Interaction mechanisms of human serum albumin (HSA) with safranal and crocin were studied using UV–Vis absorption, fluorescence quenching and circular dichroism (CD) spectroscopies as well as molecular docking techniques. Changes in absorbance and fluorescence of HSA upon interactions with both compounds were attributed to their binding to amino acid chromophores located in subdomains IIA and IIIA. Fluorescence secondary inner filter effect was excluded using 278 nm and 340 nm as the wavelengths of HSA's excitation and fluorescence while safranal and crocin absorbed at 320 nm and 445 nm, respectively. Stern-Volmer model revealed a static quenching mechanism involve the formation of non-fluorescent ground state complexes. Stern-Volmer, Hill, Benesi-Hilbrand and Scatchard models gave apparent binding constants ranged in 4.25 × 103 - 2.15 × 105 for safranal and 7.67 × 103 - 4.23 × 105 L mol−1 for crocin. CD measurements indicated that 13 folds of safranal and crocin unfolded the α-helix structure of HSA by 7.47–21.20%. In-silico molecular docking revealed selective exothermic binding of safranal on eight binding sites with binding energies ranged in −3.969 to −6.6.913 kcal/mol. Crocin exothermally bound to a new large pocket located on subdomain IIA (sudlow 1) with binding energy of −12.922 kcal/mol.These results confirmed the formation of HSA stable complexes with safranal and crocin and contributed to our understanding for their binding characteristics (affinities, sites, modes, forces … etc.) and structural changes upon interactions. They also proved that HSA can solubilize and transport both compounds in blood to target tissues. The results are of high importance in determining the pharmacological properties of the two phytochemical compounds and for their future developments as anticancer, antispasmodic, antidepressant or aphrodisiac therapeutic agents. Keywords: Human serum albumin. safranal, Crocin, UV–Vis, Fluorescence quenching, Circular dichroism, Molecular docking |
| first_indexed | 2024-12-11T10:03:48Z |
| format | Article |
| id | doaj.art-cf286d088e6b47a3a3f5861d957a7e96 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-11T10:03:48Z |
| publishDate | 2019-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-cf286d088e6b47a3a3f5861d957a7e962022-12-22T01:12:03ZengElsevierBiochemistry and Biophysics Reports2405-58082019-12-0120Characterization of human serum albumin's interactions with safranal and crocin using multi-spectroscopic and molecular docking techniquesAlaa A. Salem0Mohamed Lotfy1Amr Amin2Mohammad A. Ghattas3Department of Chemistry, College of Science, United Arab Emirates University, Al Ain, P.O. Box 15551, United Arab Emirates; Corresponding author.Department of Biology, College of Science, United Arab Emirates University, Al Ain, P.O. Box 1551, United Arab EmiratesDepartment of Biology, College of Science, United Arab Emirates University, Al Ain, P.O. Box 1551, United Arab EmiratesCollege of Pharmacy, Al Ain University of Science and Technology, P.O. Box 112612, Abu Dhabi, United Arab EmiratesInteraction mechanisms of human serum albumin (HSA) with safranal and crocin were studied using UV–Vis absorption, fluorescence quenching and circular dichroism (CD) spectroscopies as well as molecular docking techniques. Changes in absorbance and fluorescence of HSA upon interactions with both compounds were attributed to their binding to amino acid chromophores located in subdomains IIA and IIIA. Fluorescence secondary inner filter effect was excluded using 278 nm and 340 nm as the wavelengths of HSA's excitation and fluorescence while safranal and crocin absorbed at 320 nm and 445 nm, respectively. Stern-Volmer model revealed a static quenching mechanism involve the formation of non-fluorescent ground state complexes. Stern-Volmer, Hill, Benesi-Hilbrand and Scatchard models gave apparent binding constants ranged in 4.25 × 103 - 2.15 × 105 for safranal and 7.67 × 103 - 4.23 × 105 L mol−1 for crocin. CD measurements indicated that 13 folds of safranal and crocin unfolded the α-helix structure of HSA by 7.47–21.20%. In-silico molecular docking revealed selective exothermic binding of safranal on eight binding sites with binding energies ranged in −3.969 to −6.6.913 kcal/mol. Crocin exothermally bound to a new large pocket located on subdomain IIA (sudlow 1) with binding energy of −12.922 kcal/mol.These results confirmed the formation of HSA stable complexes with safranal and crocin and contributed to our understanding for their binding characteristics (affinities, sites, modes, forces … etc.) and structural changes upon interactions. They also proved that HSA can solubilize and transport both compounds in blood to target tissues. The results are of high importance in determining the pharmacological properties of the two phytochemical compounds and for their future developments as anticancer, antispasmodic, antidepressant or aphrodisiac therapeutic agents. Keywords: Human serum albumin. safranal, Crocin, UV–Vis, Fluorescence quenching, Circular dichroism, Molecular dockinghttp://www.sciencedirect.com/science/article/pii/S2405580819300822 |
| spellingShingle | Alaa A. Salem Mohamed Lotfy Amr Amin Mohammad A. Ghattas Characterization of human serum albumin's interactions with safranal and crocin using multi-spectroscopic and molecular docking techniques Biochemistry and Biophysics Reports |
| title | Characterization of human serum albumin's interactions with safranal and crocin using multi-spectroscopic and molecular docking techniques |
| title_full | Characterization of human serum albumin's interactions with safranal and crocin using multi-spectroscopic and molecular docking techniques |
| title_fullStr | Characterization of human serum albumin's interactions with safranal and crocin using multi-spectroscopic and molecular docking techniques |
| title_full_unstemmed | Characterization of human serum albumin's interactions with safranal and crocin using multi-spectroscopic and molecular docking techniques |
| title_short | Characterization of human serum albumin's interactions with safranal and crocin using multi-spectroscopic and molecular docking techniques |
| title_sort | characterization of human serum albumin s interactions with safranal and crocin using multi spectroscopic and molecular docking techniques |
| url | http://www.sciencedirect.com/science/article/pii/S2405580819300822 |
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