Single‐cell Raman and functional gene analyses reveal microbial P solubilization in agriculture waste‐modified soils
Abstract Application of agricultural waste such as rapeseed meal (RM) is regarded as a sustainable way to improve soil phosphorus (P) availability by direct nutrient supply and stimulation of native phosphate‐solubilizing microorganisms (PSMs) in soils. However, exploration of the in situ microbial...
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Wiley
2023-06-01
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| Series: | mLife |
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| Online Access: | https://doi.org/10.1002/mlf2.12053 |
| _version_ | 1797791688225193984 |
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| author | Hongzhe Li Jiazhi Ding Longji Zhu Fei Xu Wenjing Li Yanpo Yao Li Cui |
| author_facet | Hongzhe Li Jiazhi Ding Longji Zhu Fei Xu Wenjing Li Yanpo Yao Li Cui |
| author_sort | Hongzhe Li |
| collection | DOAJ |
| description | Abstract Application of agricultural waste such as rapeseed meal (RM) is regarded as a sustainable way to improve soil phosphorus (P) availability by direct nutrient supply and stimulation of native phosphate‐solubilizing microorganisms (PSMs) in soils. However, exploration of the in situ microbial P solubilizing function in soils remains a challenge. Here, by applying both phenotype‐based single‐cell Raman with D2O labeling (Raman‐D2O) and genotype‐based high‐throughput chips targeting carbon, nitrogen and P (CNP) functional genes, the effect of RM application on microbial P solubilization in three typical farmland soils was investigated. The abundances of PSMs increased in two alkaline soils after RM application identified by single‐cell Raman D2O. RM application reduced the diversity of bacterial communities and increased the abundance of a few bacteria with reported P solubilization function. Genotypic analysis indicated that RM addition generally increased the relative abundance of CNP functional genes. A correlation analysis of the abundance of active PSMs with the abundance of soil microbes or functional genes was carried out to decipher the linkage between the phenotype and genotype of PSMs. Myxococcota and C degradation genes were found to potentially contribute to the enhanced microbial P release following RM application. This work provides important new insights into the in situ function of soil PSMs. It will lead to better harnessing of agricultural waste to mobilize soil legacy P and mitigate the P crisis. |
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| id | doaj.art-cf2fe716b2ab4ff0b1fd090fcb5d85ab |
| institution | Directory Open Access Journal |
| issn | 2770-100X |
| language | English |
| last_indexed | 2024-03-13T02:22:23Z |
| publishDate | 2023-06-01 |
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| spelling | doaj.art-cf2fe716b2ab4ff0b1fd090fcb5d85ab2023-06-30T08:36:29ZengWileymLife2770-100X2023-06-012219020010.1002/mlf2.12053Single‐cell Raman and functional gene analyses reveal microbial P solubilization in agriculture waste‐modified soilsHongzhe Li0Jiazhi Ding1Longji Zhu2Fei Xu3Wenjing Li4Yanpo Yao5Li Cui6Key Lab of Urban Environment and Health Institute of Urban Environment, Chinese Academy of Sciences Xiamen ChinaKey Lab of Urban Environment and Health Institute of Urban Environment, Chinese Academy of Sciences Xiamen ChinaKey Lab of Urban Environment and Health Institute of Urban Environment, Chinese Academy of Sciences Xiamen ChinaKey Lab of Urban Environment and Health Institute of Urban Environment, Chinese Academy of Sciences Xiamen ChinaKey Lab of Urban Environment and Health Institute of Urban Environment, Chinese Academy of Sciences Xiamen ChinaAgro‐Environmental Protection Institute Ministry of Agriculture and Rural Affairs Tianjin ChinaKey Lab of Urban Environment and Health Institute of Urban Environment, Chinese Academy of Sciences Xiamen ChinaAbstract Application of agricultural waste such as rapeseed meal (RM) is regarded as a sustainable way to improve soil phosphorus (P) availability by direct nutrient supply and stimulation of native phosphate‐solubilizing microorganisms (PSMs) in soils. However, exploration of the in situ microbial P solubilizing function in soils remains a challenge. Here, by applying both phenotype‐based single‐cell Raman with D2O labeling (Raman‐D2O) and genotype‐based high‐throughput chips targeting carbon, nitrogen and P (CNP) functional genes, the effect of RM application on microbial P solubilization in three typical farmland soils was investigated. The abundances of PSMs increased in two alkaline soils after RM application identified by single‐cell Raman D2O. RM application reduced the diversity of bacterial communities and increased the abundance of a few bacteria with reported P solubilization function. Genotypic analysis indicated that RM addition generally increased the relative abundance of CNP functional genes. A correlation analysis of the abundance of active PSMs with the abundance of soil microbes or functional genes was carried out to decipher the linkage between the phenotype and genotype of PSMs. Myxococcota and C degradation genes were found to potentially contribute to the enhanced microbial P release following RM application. This work provides important new insights into the in situ function of soil PSMs. It will lead to better harnessing of agricultural waste to mobilize soil legacy P and mitigate the P crisis.https://doi.org/10.1002/mlf2.12053CNP functional genesD2O isotope labelingphosphate‐solubilizing microorganismssingle‐cell Raman |
| spellingShingle | Hongzhe Li Jiazhi Ding Longji Zhu Fei Xu Wenjing Li Yanpo Yao Li Cui Single‐cell Raman and functional gene analyses reveal microbial P solubilization in agriculture waste‐modified soils mLife CNP functional genes D2O isotope labeling phosphate‐solubilizing microorganisms single‐cell Raman |
| title | Single‐cell Raman and functional gene analyses reveal microbial P solubilization in agriculture waste‐modified soils |
| title_full | Single‐cell Raman and functional gene analyses reveal microbial P solubilization in agriculture waste‐modified soils |
| title_fullStr | Single‐cell Raman and functional gene analyses reveal microbial P solubilization in agriculture waste‐modified soils |
| title_full_unstemmed | Single‐cell Raman and functional gene analyses reveal microbial P solubilization in agriculture waste‐modified soils |
| title_short | Single‐cell Raman and functional gene analyses reveal microbial P solubilization in agriculture waste‐modified soils |
| title_sort | single cell raman and functional gene analyses reveal microbial p solubilization in agriculture waste modified soils |
| topic | CNP functional genes D2O isotope labeling phosphate‐solubilizing microorganisms single‐cell Raman |
| url | https://doi.org/10.1002/mlf2.12053 |
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