Genistein treatment increases bone mass in obese, hyperglycemic mice
Richard M Michelin,1 Layla Al-Nakkash,2 Tom L Broderick,3 Jeffrey H Plochocki4 1Arizona College of Osteopathic Medicine, 2Department of Physiology, 3Laboratory of Diabetes and Exercise Metabolism, Department of Physiology, 4Department of Anatomy, Arizona College of Osteopathic Medicine, Midwestern U...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2016-03-01
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| Series: | Diabetes, Metabolic Syndrome and Obesity |
| Subjects: | |
| Online Access: | https://www.dovepress.com/genistein-treatment-increases-bone-mass-in-obese-hyperglycemic-mice-peer-reviewed-article-DMSO |
| _version_ | 1797935277943029760 |
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| author | Michelin RM Al-Nakkash L Broderick TL Plochocki JH |
| author_facet | Michelin RM Al-Nakkash L Broderick TL Plochocki JH |
| author_sort | Michelin RM |
| collection | DOAJ |
| description | Richard M Michelin,1 Layla Al-Nakkash,2 Tom L Broderick,3 Jeffrey H Plochocki4 1Arizona College of Osteopathic Medicine, 2Department of Physiology, 3Laboratory of Diabetes and Exercise Metabolism, Department of Physiology, 4Department of Anatomy, Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ, USA Background: Obesity and type 2 diabetes mellitus are associated with elevated risk of limb bone fracture. Incidences of these conditions are on the rise worldwide. Genistein, a phytoestrogen, has been shown by several studies to demonstrate bone-protective properties and may improve bone health in obese type 2 diabetics. Methods: In this study, we test the effects of genistein treatment on limb bone and growth plate cartilage histomorphometry in obese, hyperglycemic ob/ob mice. Six-week-old ob/ob mice were divided into control and genistein-treated groups. Genistein-treated mice were fed a diet containing 600 mg genistein/kg for a period of 4 weeks. Cross-sectional geometric and histomorphometric analyses were conducted on tibias. Results: Genistein-treated mice remained obese and hyperglycemic. However, histomorphometric comparisons show that genistein-treated mice have greater tibial midshaft diameters and ratios of cortical bone to total tissue area than the controls. Genistein-treated mice also exhibit decreased growth plate thickness of the proximal tibia. Conclusion: Our results indicate that genistein treatment affects bone of the tibial midshaft in the ob/ob mouse, independent of improvements in the hyperglycemic state and body weight. Keywords: obesity, hyperglycemia, genistein, ob/ob mice, bone |
| first_indexed | 2024-04-10T18:13:03Z |
| format | Article |
| id | doaj.art-cf34d402de0642e99c1d404633b304c0 |
| institution | Directory Open Access Journal |
| issn | 1178-7007 |
| language | English |
| last_indexed | 2024-04-10T18:13:03Z |
| publishDate | 2016-03-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Diabetes, Metabolic Syndrome and Obesity |
| spelling | doaj.art-cf34d402de0642e99c1d404633b304c02023-02-02T10:28:09ZengDove Medical PressDiabetes, Metabolic Syndrome and Obesity1178-70072016-03-012016Issue 1637025990Genistein treatment increases bone mass in obese, hyperglycemic miceMichelin RMAl-Nakkash LBroderick TLPlochocki JHRichard M Michelin,1 Layla Al-Nakkash,2 Tom L Broderick,3 Jeffrey H Plochocki4 1Arizona College of Osteopathic Medicine, 2Department of Physiology, 3Laboratory of Diabetes and Exercise Metabolism, Department of Physiology, 4Department of Anatomy, Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ, USA Background: Obesity and type 2 diabetes mellitus are associated with elevated risk of limb bone fracture. Incidences of these conditions are on the rise worldwide. Genistein, a phytoestrogen, has been shown by several studies to demonstrate bone-protective properties and may improve bone health in obese type 2 diabetics. Methods: In this study, we test the effects of genistein treatment on limb bone and growth plate cartilage histomorphometry in obese, hyperglycemic ob/ob mice. Six-week-old ob/ob mice were divided into control and genistein-treated groups. Genistein-treated mice were fed a diet containing 600 mg genistein/kg for a period of 4 weeks. Cross-sectional geometric and histomorphometric analyses were conducted on tibias. Results: Genistein-treated mice remained obese and hyperglycemic. However, histomorphometric comparisons show that genistein-treated mice have greater tibial midshaft diameters and ratios of cortical bone to total tissue area than the controls. Genistein-treated mice also exhibit decreased growth plate thickness of the proximal tibia. Conclusion: Our results indicate that genistein treatment affects bone of the tibial midshaft in the ob/ob mouse, independent of improvements in the hyperglycemic state and body weight. Keywords: obesity, hyperglycemia, genistein, ob/ob mice, bonehttps://www.dovepress.com/genistein-treatment-increases-bone-mass-in-obese-hyperglycemic-mice-peer-reviewed-article-DMSOobesityhyperglycemiagenisteinob/ob micebone |
| spellingShingle | Michelin RM Al-Nakkash L Broderick TL Plochocki JH Genistein treatment increases bone mass in obese, hyperglycemic mice Diabetes, Metabolic Syndrome and Obesity obesity hyperglycemia genistein ob/ob mice bone |
| title | Genistein treatment increases bone mass in obese, hyperglycemic mice |
| title_full | Genistein treatment increases bone mass in obese, hyperglycemic mice |
| title_fullStr | Genistein treatment increases bone mass in obese, hyperglycemic mice |
| title_full_unstemmed | Genistein treatment increases bone mass in obese, hyperglycemic mice |
| title_short | Genistein treatment increases bone mass in obese, hyperglycemic mice |
| title_sort | genistein treatment increases bone mass in obese hyperglycemic mice |
| topic | obesity hyperglycemia genistein ob/ob mice bone |
| url | https://www.dovepress.com/genistein-treatment-increases-bone-mass-in-obese-hyperglycemic-mice-peer-reviewed-article-DMSO |
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