Anti-inflammaging effects of vitamin D in human gingival fibroblasts with advanced glycation end product stimulation
Background/purpose: :Both periodontal disease and diabetes mellitus (DM) are long-term inflammatory disorders that are highly prevalent and have a significant health impact. Inflammaging, a state of pre-aging and hyperinflammatory state has been acknowledged for its role in DM patients to have heigh...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Journal of Dental Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790222002550 |
| _version_ | 1797862208028278784 |
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| author | Hung-Chieh Lu Taichen Lin Min Yee Ng Chang-Wei Hsieh Yi-Wen Liao Chun-Cheng Chen Cheng-Chia Yu Chun-Jung Chen |
| author_facet | Hung-Chieh Lu Taichen Lin Min Yee Ng Chang-Wei Hsieh Yi-Wen Liao Chun-Cheng Chen Cheng-Chia Yu Chun-Jung Chen |
| author_sort | Hung-Chieh Lu |
| collection | DOAJ |
| description | Background/purpose: :Both periodontal disease and diabetes mellitus (DM) are long-term inflammatory disorders that are highly prevalent and have a significant health impact. Inflammaging, a state of pre-aging and hyperinflammatory state has been acknowledged for its role in DM patients to have heightened risk of periodontitis. Numerous evidences revealed that inflammaging contributed by cell senescence, acceleration of inflammation and oxidative stress participates in the destruction of periodontium in DM. Abilities of vitamin D in suppressing inflammation and oxidative stress have been revealed in a range of tissues, however in DM’s gingival cells, the effect remain undefined. Materials and methods: : Under the stimulation of advanced glycation end-products (AGEs), we assessed the cell proliferation in human gingival fibroblast (HGF), IL-6 and IL-8 secretions, cellular senescence expression and generation of reactive oxygen species (ROS) with or without vitamin D intervention. Following that, we examined the expression of Nrf2 and HO-1 to see if vitamin D was able to modulate the anti-oxidant signaling. A knockdown experiment was then conducted to proof the participation of Nrf2 on the secretion of pro-inflammatory IL-6 and IL-8. Results: : Following the treatment of vitamin D, AGEs-elicited IL-6 and IL-8 production and cell senescence were dose-dependently repressed. Moreover, vitamin D attenuated AGEs-induced ROS in a dose-dependent pattern. Results from qRT-PCR demonstrated vitamin D reversed the suppression of Nrf2 and HO-1 induced by AGEs. Our findings revealed that the anti-inflammatory and anti-oxidant effect in vitamin D was mediated via the upregulation of Nrf2 expression. Conclusion: : These data showed that high levels of AGEs in the gingiva lead to inflammaging reflected by increased pro-inflammatory cytokines, cell senescence expression and oxidative stress. Vitamin D supplementation can reduce oxidative stress and inflammation via the upregulation of Nrf2 signaling and hence, may be a potential approach for treatment of diabetes-associated periodontitis. |
| first_indexed | 2024-04-09T22:15:32Z |
| format | Article |
| id | doaj.art-cf35421663f941d18ea62a52c9040260 |
| institution | Directory Open Access Journal |
| issn | 1991-7902 |
| language | English |
| last_indexed | 2024-04-09T22:15:32Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dental Sciences |
| spelling | doaj.art-cf35421663f941d18ea62a52c90402602023-03-23T04:35:05ZengElsevierJournal of Dental Sciences1991-79022023-04-01182666673Anti-inflammaging effects of vitamin D in human gingival fibroblasts with advanced glycation end product stimulationHung-Chieh Lu0Taichen Lin1Min Yee Ng2Chang-Wei Hsieh3Yi-Wen Liao4Chun-Cheng Chen5Cheng-Chia Yu6Chun-Jung Chen7School of Dentistry, Chung Shan Medical University, Taichung, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung, Taiwan; Department of Dentistry, Chung Shan Medical University Hospital, Taichung, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung, TaiwanDepartment of Food Science and Biotechnology, National Chung Hsing University, Taichung, TaiwanDepartment of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan; Institute of Oral Sciences, Chung Shan Medical University, Taichung, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung, Taiwan; Department of Dentistry, Chung Shan Medical University Hospital, Taichung, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung, Taiwan; Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan; Institute of Oral Sciences, Chung Shan Medical University, Taichung, Taiwan; Corresponding author. Institute of Oral Sciences, Chung Shan Medical University, No.110, Sec.1, Jianguo N. Rd., Taichung 40201, Taiwan.School of Dentistry, Chung Shan Medical University, Taichung, Taiwan; Division of Periodontics, Department of Dentistry, Chi Mei Medical Center, Tainan, Taiwan; Corresponding author. Division of Periodontics, Department of Dentistry, Chi Mei Medical Center, No. 901, Zhonghua Rd. Yongkang Dist., Tainan 71004, Taiwan.Background/purpose: :Both periodontal disease and diabetes mellitus (DM) are long-term inflammatory disorders that are highly prevalent and have a significant health impact. Inflammaging, a state of pre-aging and hyperinflammatory state has been acknowledged for its role in DM patients to have heightened risk of periodontitis. Numerous evidences revealed that inflammaging contributed by cell senescence, acceleration of inflammation and oxidative stress participates in the destruction of periodontium in DM. Abilities of vitamin D in suppressing inflammation and oxidative stress have been revealed in a range of tissues, however in DM’s gingival cells, the effect remain undefined. Materials and methods: : Under the stimulation of advanced glycation end-products (AGEs), we assessed the cell proliferation in human gingival fibroblast (HGF), IL-6 and IL-8 secretions, cellular senescence expression and generation of reactive oxygen species (ROS) with or without vitamin D intervention. Following that, we examined the expression of Nrf2 and HO-1 to see if vitamin D was able to modulate the anti-oxidant signaling. A knockdown experiment was then conducted to proof the participation of Nrf2 on the secretion of pro-inflammatory IL-6 and IL-8. Results: : Following the treatment of vitamin D, AGEs-elicited IL-6 and IL-8 production and cell senescence were dose-dependently repressed. Moreover, vitamin D attenuated AGEs-induced ROS in a dose-dependent pattern. Results from qRT-PCR demonstrated vitamin D reversed the suppression of Nrf2 and HO-1 induced by AGEs. Our findings revealed that the anti-inflammatory and anti-oxidant effect in vitamin D was mediated via the upregulation of Nrf2 expression. Conclusion: : These data showed that high levels of AGEs in the gingiva lead to inflammaging reflected by increased pro-inflammatory cytokines, cell senescence expression and oxidative stress. Vitamin D supplementation can reduce oxidative stress and inflammation via the upregulation of Nrf2 signaling and hence, may be a potential approach for treatment of diabetes-associated periodontitis.http://www.sciencedirect.com/science/article/pii/S1991790222002550PeriodontitisAdvanced glycation end productsVitamin D |
| spellingShingle | Hung-Chieh Lu Taichen Lin Min Yee Ng Chang-Wei Hsieh Yi-Wen Liao Chun-Cheng Chen Cheng-Chia Yu Chun-Jung Chen Anti-inflammaging effects of vitamin D in human gingival fibroblasts with advanced glycation end product stimulation Journal of Dental Sciences Periodontitis Advanced glycation end products Vitamin D |
| title | Anti-inflammaging effects of vitamin D in human gingival fibroblasts with advanced glycation end product stimulation |
| title_full | Anti-inflammaging effects of vitamin D in human gingival fibroblasts with advanced glycation end product stimulation |
| title_fullStr | Anti-inflammaging effects of vitamin D in human gingival fibroblasts with advanced glycation end product stimulation |
| title_full_unstemmed | Anti-inflammaging effects of vitamin D in human gingival fibroblasts with advanced glycation end product stimulation |
| title_short | Anti-inflammaging effects of vitamin D in human gingival fibroblasts with advanced glycation end product stimulation |
| title_sort | anti inflammaging effects of vitamin d in human gingival fibroblasts with advanced glycation end product stimulation |
| topic | Periodontitis Advanced glycation end products Vitamin D |
| url | http://www.sciencedirect.com/science/article/pii/S1991790222002550 |
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