Role of Snf-β in lipid accumulation in the high lipid‐producing fungus Mucor circinelloides WJ11
Abstract Background Mucor circinelloides WJ11 is a high-lipid producing strain and an excellent producer of γ-linolenic acid (GLA) which is crucial for human health. We have previously identified genes that encode for AMP-activated protein kinase (AMPK) complex in M. circinelloides which is an impor...
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| Format: | Article |
| Language: | English |
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BMC
2021-02-01
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| Series: | Microbial Cell Factories |
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| Online Access: | https://doi.org/10.1186/s12934-021-01545-y |
| _version_ | 1818618349759758336 |
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| author | Shaista Nosheen Tahira Naz Junhuan Yang Syed Ammar Hussain Abu Bakr Ahmad Fazili Yusuf Nazir Shaoqi Li Hassan Mohamed Wu Yang Kiren Mustafa Yuanda Song |
| author_facet | Shaista Nosheen Tahira Naz Junhuan Yang Syed Ammar Hussain Abu Bakr Ahmad Fazili Yusuf Nazir Shaoqi Li Hassan Mohamed Wu Yang Kiren Mustafa Yuanda Song |
| author_sort | Shaista Nosheen |
| collection | DOAJ |
| description | Abstract Background Mucor circinelloides WJ11 is a high-lipid producing strain and an excellent producer of γ-linolenic acid (GLA) which is crucial for human health. We have previously identified genes that encode for AMP-activated protein kinase (AMPK) complex in M. circinelloides which is an important regulator for lipid accumulation. Comparative transcriptional analysis between the high and low lipid-producing strains of M. circinelloides showed a direct correlation in the transcriptional level of AMPK genes with lipid metabolism. Thus, the role of Snf-β, which encodes for β subunit of AMPK complex, in lipid accumulation of the WJ11 strain was evaluated in the present study. Results The results showed that lipid content of cell dry weight in Snf-β knockout strain was increased by 32 % (from 19 to 25 %). However, in Snf-β overexpressing strain, lipid content of cell dry weight was decreased about 25 % (from 19 to 14.2 %) compared to the control strain. Total fatty acid analysis revealed that the expression of the Snf-β gene did not significantly affect the fatty acid composition of the strains. However, GLA content in biomass was increased from 2.5 % in control strain to 3.3 % in Snf-β knockout strain due to increased lipid accumulation and decreased to 1.83 % in Snf-β overexpressing strain. AMPK is known to inactivate acetyl-CoA carboxylase (ACC) which catalyzes the rate-limiting step in lipid synthesis. Snf-β manipulation also altered the expression level of the ACC1 gene which may indicate that Snf-β control lipid metabolism by regulating ACC1 gene. Conclusions Our results suggested that Snf-β gene plays an important role in regulating lipid accumulation in M. circinelloides WJ11. Moreover, it will be interesting to evaluate the potential of other key subunits of AMPK related to lipid metabolism. Better insight can show us the way to manipulate these subunits effectively for upscaling the lipid production. Up to our knowledge, it is the first study to investigate the role of Snf-β in lipid accumulation in M. circinelloides. |
| first_indexed | 2024-12-16T17:20:11Z |
| format | Article |
| id | doaj.art-cf3690bccb4f4c69991913e7f7b60aeb |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-16T17:20:11Z |
| publishDate | 2021-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-cf3690bccb4f4c69991913e7f7b60aeb2022-12-21T22:23:11ZengBMCMicrobial Cell Factories1475-28592021-02-0120111110.1186/s12934-021-01545-yRole of Snf-β in lipid accumulation in the high lipid‐producing fungus Mucor circinelloides WJ11Shaista Nosheen0Tahira Naz1Junhuan Yang2Syed Ammar Hussain3Abu Bakr Ahmad Fazili4Yusuf Nazir5Shaoqi Li6Hassan Mohamed7Wu Yang8Kiren Mustafa9Yuanda Song10Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of TechnologyColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of TechnologyColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of TechnologyColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of TechnologyColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of TechnologyColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of TechnologyColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of TechnologyColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of TechnologyColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of TechnologyColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of TechnologyColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of TechnologyAbstract Background Mucor circinelloides WJ11 is a high-lipid producing strain and an excellent producer of γ-linolenic acid (GLA) which is crucial for human health. We have previously identified genes that encode for AMP-activated protein kinase (AMPK) complex in M. circinelloides which is an important regulator for lipid accumulation. Comparative transcriptional analysis between the high and low lipid-producing strains of M. circinelloides showed a direct correlation in the transcriptional level of AMPK genes with lipid metabolism. Thus, the role of Snf-β, which encodes for β subunit of AMPK complex, in lipid accumulation of the WJ11 strain was evaluated in the present study. Results The results showed that lipid content of cell dry weight in Snf-β knockout strain was increased by 32 % (from 19 to 25 %). However, in Snf-β overexpressing strain, lipid content of cell dry weight was decreased about 25 % (from 19 to 14.2 %) compared to the control strain. Total fatty acid analysis revealed that the expression of the Snf-β gene did not significantly affect the fatty acid composition of the strains. However, GLA content in biomass was increased from 2.5 % in control strain to 3.3 % in Snf-β knockout strain due to increased lipid accumulation and decreased to 1.83 % in Snf-β overexpressing strain. AMPK is known to inactivate acetyl-CoA carboxylase (ACC) which catalyzes the rate-limiting step in lipid synthesis. Snf-β manipulation also altered the expression level of the ACC1 gene which may indicate that Snf-β control lipid metabolism by regulating ACC1 gene. Conclusions Our results suggested that Snf-β gene plays an important role in regulating lipid accumulation in M. circinelloides WJ11. Moreover, it will be interesting to evaluate the potential of other key subunits of AMPK related to lipid metabolism. Better insight can show us the way to manipulate these subunits effectively for upscaling the lipid production. Up to our knowledge, it is the first study to investigate the role of Snf-β in lipid accumulation in M. circinelloides.https://doi.org/10.1186/s12934-021-01545-yMucor circinelloidesLipid accumulationSnf-βacetyl-CoA carboxylaseGene expressionOleaginous fungus |
| spellingShingle | Shaista Nosheen Tahira Naz Junhuan Yang Syed Ammar Hussain Abu Bakr Ahmad Fazili Yusuf Nazir Shaoqi Li Hassan Mohamed Wu Yang Kiren Mustafa Yuanda Song Role of Snf-β in lipid accumulation in the high lipid‐producing fungus Mucor circinelloides WJ11 Microbial Cell Factories Mucor circinelloides Lipid accumulation Snf-β acetyl-CoA carboxylase Gene expression Oleaginous fungus |
| title | Role of Snf-β in lipid accumulation in the high lipid‐producing fungus Mucor circinelloides WJ11 |
| title_full | Role of Snf-β in lipid accumulation in the high lipid‐producing fungus Mucor circinelloides WJ11 |
| title_fullStr | Role of Snf-β in lipid accumulation in the high lipid‐producing fungus Mucor circinelloides WJ11 |
| title_full_unstemmed | Role of Snf-β in lipid accumulation in the high lipid‐producing fungus Mucor circinelloides WJ11 |
| title_short | Role of Snf-β in lipid accumulation in the high lipid‐producing fungus Mucor circinelloides WJ11 |
| title_sort | role of snf β in lipid accumulation in the high lipid producing fungus mucor circinelloides wj11 |
| topic | Mucor circinelloides Lipid accumulation Snf-β acetyl-CoA carboxylase Gene expression Oleaginous fungus |
| url | https://doi.org/10.1186/s12934-021-01545-y |
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