High CD3+ Cells in Intracranial Thrombi Represent a Biomarker of Atherothrombotic Stroke.
BACKGROUND AND PURPOSE:Approximately 30% of strokes are cryptogenic despite an exhaustive in-hospital work-up. Analysis of clot composition following endovascular treatment could provide insight into stroke etiology. T-cells already have been shown to be a major component of vulnerable atherosclerot...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4859469?pdf=render |
| _version_ | 1818838810419527680 |
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| author | Cyril Dargazanli Valérie Rigau Omer Eker Carlos Riquelme Bareiro Paolo Machi Grégory Gascou Caroline Arquizan Xavier Ayrignac Isabelle Mourand Astrid Corlobé Kyriakos Lobotesis Nicolas Molinari Valérie Costes Alain Bonafé Vincent Costalat |
| author_facet | Cyril Dargazanli Valérie Rigau Omer Eker Carlos Riquelme Bareiro Paolo Machi Grégory Gascou Caroline Arquizan Xavier Ayrignac Isabelle Mourand Astrid Corlobé Kyriakos Lobotesis Nicolas Molinari Valérie Costes Alain Bonafé Vincent Costalat |
| author_sort | Cyril Dargazanli |
| collection | DOAJ |
| description | BACKGROUND AND PURPOSE:Approximately 30% of strokes are cryptogenic despite an exhaustive in-hospital work-up. Analysis of clot composition following endovascular treatment could provide insight into stroke etiology. T-cells already have been shown to be a major component of vulnerable atherosclerotic carotid lesions. We therefore hypothesize that T-cell content in intracranial thrombi may also be a biomarker of atherothrombotic origin. MATERIALS AND METHODS:We histopathologically investigated 54 consecutive thrombi retrieved after mechanical thrombectomy in acute stroke patients. First, thrombi were classified as fibrin-dominant, erythrocyte-dominant or mixed pattern. We then performed quantitative analysis of CD3+ cells on immunohistochemically-stained thrombi and compared T-cell content between "atherothrombotic", "cardioembolism" and "other causes" stroke subtypes. RESULTS:Fourteen (26%) thrombi were defined as fibrin-dominant, 15 (28%) as erythrocyte-dominant, 25 (46%) as mixed. The stroke cause was defined as "atherothrombotic" in 10 (18.5%), "cardioembolism" in 25 (46.3%), and "other causes" in 19 (35.2%). Number of T-cells was significantly higher in thrombi from the "atherothrombotic" group (53.60 ± 28.78) than in the other causes (21.77 ± 18.31; p<0.0005) or the "cardioembolism" group (20.08 ± 15.66; p<0.0003). CONCLUSIONS:The CD3+ T-cell count in intracranial thrombi was significantly higher in "atherothrombotic" origin strokes compared to all other causes. Thrombi with high content of CD3+ cells are more likely to originate from an atherosclerotic plaque. |
| first_indexed | 2024-12-19T03:44:19Z |
| format | Article |
| id | doaj.art-cf3e50fa0d0c4a45915e24a4cf24f869 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T03:44:19Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-cf3e50fa0d0c4a45915e24a4cf24f8692022-12-21T20:37:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01115e015494510.1371/journal.pone.0154945High CD3+ Cells in Intracranial Thrombi Represent a Biomarker of Atherothrombotic Stroke.Cyril DargazanliValérie RigauOmer EkerCarlos Riquelme BareiroPaolo MachiGrégory GascouCaroline ArquizanXavier AyrignacIsabelle MourandAstrid CorlobéKyriakos LobotesisNicolas MolinariValérie CostesAlain BonaféVincent CostalatBACKGROUND AND PURPOSE:Approximately 30% of strokes are cryptogenic despite an exhaustive in-hospital work-up. Analysis of clot composition following endovascular treatment could provide insight into stroke etiology. T-cells already have been shown to be a major component of vulnerable atherosclerotic carotid lesions. We therefore hypothesize that T-cell content in intracranial thrombi may also be a biomarker of atherothrombotic origin. MATERIALS AND METHODS:We histopathologically investigated 54 consecutive thrombi retrieved after mechanical thrombectomy in acute stroke patients. First, thrombi were classified as fibrin-dominant, erythrocyte-dominant or mixed pattern. We then performed quantitative analysis of CD3+ cells on immunohistochemically-stained thrombi and compared T-cell content between "atherothrombotic", "cardioembolism" and "other causes" stroke subtypes. RESULTS:Fourteen (26%) thrombi were defined as fibrin-dominant, 15 (28%) as erythrocyte-dominant, 25 (46%) as mixed. The stroke cause was defined as "atherothrombotic" in 10 (18.5%), "cardioembolism" in 25 (46.3%), and "other causes" in 19 (35.2%). Number of T-cells was significantly higher in thrombi from the "atherothrombotic" group (53.60 ± 28.78) than in the other causes (21.77 ± 18.31; p<0.0005) or the "cardioembolism" group (20.08 ± 15.66; p<0.0003). CONCLUSIONS:The CD3+ T-cell count in intracranial thrombi was significantly higher in "atherothrombotic" origin strokes compared to all other causes. Thrombi with high content of CD3+ cells are more likely to originate from an atherosclerotic plaque.http://europepmc.org/articles/PMC4859469?pdf=render |
| spellingShingle | Cyril Dargazanli Valérie Rigau Omer Eker Carlos Riquelme Bareiro Paolo Machi Grégory Gascou Caroline Arquizan Xavier Ayrignac Isabelle Mourand Astrid Corlobé Kyriakos Lobotesis Nicolas Molinari Valérie Costes Alain Bonafé Vincent Costalat High CD3+ Cells in Intracranial Thrombi Represent a Biomarker of Atherothrombotic Stroke. PLoS ONE |
| title | High CD3+ Cells in Intracranial Thrombi Represent a Biomarker of Atherothrombotic Stroke. |
| title_full | High CD3+ Cells in Intracranial Thrombi Represent a Biomarker of Atherothrombotic Stroke. |
| title_fullStr | High CD3+ Cells in Intracranial Thrombi Represent a Biomarker of Atherothrombotic Stroke. |
| title_full_unstemmed | High CD3+ Cells in Intracranial Thrombi Represent a Biomarker of Atherothrombotic Stroke. |
| title_short | High CD3+ Cells in Intracranial Thrombi Represent a Biomarker of Atherothrombotic Stroke. |
| title_sort | high cd3 cells in intracranial thrombi represent a biomarker of atherothrombotic stroke |
| url | http://europepmc.org/articles/PMC4859469?pdf=render |
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