Effects of vitamin D3 on expression of defensins, Toll-like receptors, and vitamin D receptor in liver, kidney, and spleen of Silky Fowl
The expression of avian β-defensins (AvBDs), Toll-like receptors (TLRs), and vitamin D receptor (VDR) following in vivo vitamin D3 injection was studied. Healthy 90-day Silky Fowls were abdominally injected with vitamin D3 or untreated. Real-time PCR analyses revealed that injection of vitamin D3 si...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2013-01-01
|
| Series: | Czech Journal of Animal Science |
| Subjects: | |
| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-201301-0002_effects-of-vitamin-d3-on-expression-of-defensins-toll-like-receptors-and-vitamin-d-receptor-in-liver-kidney.php |
| _version_ | 1797899296249479168 |
|---|---|
| author | S.M. Li L.H. Ouyang D.G. Zhou |
| author_facet | S.M. Li L.H. Ouyang D.G. Zhou |
| author_sort | S.M. Li |
| collection | DOAJ |
| description | The expression of avian β-defensins (AvBDs), Toll-like receptors (TLRs), and vitamin D receptor (VDR) following in vivo vitamin D3 injection was studied. Healthy 90-day Silky Fowls were abdominally injected with vitamin D3 or untreated. Real-time PCR analyses revealed that injection of vitamin D3 significantly (P < 0.05) up-regulated the expression of TLRs (TLR2, TLR5), VDR, AvBDs (AVBD-6, GAL-1), and 24-hydroxylase (CYP24A1) in the tissues (liver, spleen, and kidney) at various times 8-24 h post injection. These results suggest that expression of VDR, AvBDs, and TLRs seems to be induced by vitamin D3 and it was concluded that the tissues expressing TLRs and VDR respond to vitamin D3 and in turn upregulate these tissues cellular functions to synthesize AvBDs. Intraperitoneal injection of vitamin D3 likely resulted in enhancing the expression of AvBDs, TLRs, and VDR, which providedinsight into factors important for the control of the innateimmune response in the chickens. |
| first_indexed | 2024-04-10T08:26:34Z |
| format | Article |
| id | doaj.art-cf3e7170289a4593976c43eff3aa53c7 |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:26:34Z |
| publishDate | 2013-01-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Animal Science |
| spelling | doaj.art-cf3e7170289a4593976c43eff3aa53c72023-02-23T03:33:05ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092013-01-015811710.17221/6519-CJAScjs-201301-0002Effects of vitamin D3 on expression of defensins, Toll-like receptors, and vitamin D receptor in liver, kidney, and spleen of Silky FowlS.M. Li0L.H. Ouyang1D.G. Zhou2Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Science, Nanchang, P.R. ChinaInstitute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Science, Nanchang, P.R. ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Yaan, P.R. ChinaThe expression of avian β-defensins (AvBDs), Toll-like receptors (TLRs), and vitamin D receptor (VDR) following in vivo vitamin D3 injection was studied. Healthy 90-day Silky Fowls were abdominally injected with vitamin D3 or untreated. Real-time PCR analyses revealed that injection of vitamin D3 significantly (P < 0.05) up-regulated the expression of TLRs (TLR2, TLR5), VDR, AvBDs (AVBD-6, GAL-1), and 24-hydroxylase (CYP24A1) in the tissues (liver, spleen, and kidney) at various times 8-24 h post injection. These results suggest that expression of VDR, AvBDs, and TLRs seems to be induced by vitamin D3 and it was concluded that the tissues expressing TLRs and VDR respond to vitamin D3 and in turn upregulate these tissues cellular functions to synthesize AvBDs. Intraperitoneal injection of vitamin D3 likely resulted in enhancing the expression of AvBDs, TLRs, and VDR, which providedinsight into factors important for the control of the innateimmune response in the chickens.https://cjas.agriculturejournals.cz/artkey/cjs-201301-0002_effects-of-vitamin-d3-on-expression-of-defensins-toll-like-receptors-and-vitamin-d-receptor-in-liver-kidney.phpvitamin d3inductionchickenavian β-defensinsreal-time pcr |
| spellingShingle | S.M. Li L.H. Ouyang D.G. Zhou Effects of vitamin D3 on expression of defensins, Toll-like receptors, and vitamin D receptor in liver, kidney, and spleen of Silky Fowl Czech Journal of Animal Science vitamin d3 induction chicken avian β -defensins real-time pcr |
| title | Effects of vitamin D3 on expression of defensins, Toll-like receptors, and vitamin D receptor in liver, kidney, and spleen of Silky Fowl |
| title_full | Effects of vitamin D3 on expression of defensins, Toll-like receptors, and vitamin D receptor in liver, kidney, and spleen of Silky Fowl |
| title_fullStr | Effects of vitamin D3 on expression of defensins, Toll-like receptors, and vitamin D receptor in liver, kidney, and spleen of Silky Fowl |
| title_full_unstemmed | Effects of vitamin D3 on expression of defensins, Toll-like receptors, and vitamin D receptor in liver, kidney, and spleen of Silky Fowl |
| title_short | Effects of vitamin D3 on expression of defensins, Toll-like receptors, and vitamin D receptor in liver, kidney, and spleen of Silky Fowl |
| title_sort | effects of vitamin d3 on expression of defensins toll like receptors and vitamin d receptor in liver kidney and spleen of silky fowl |
| topic | vitamin d3 induction chicken avian β -defensins real-time pcr |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-201301-0002_effects-of-vitamin-d3-on-expression-of-defensins-toll-like-receptors-and-vitamin-d-receptor-in-liver-kidney.php |
| work_keys_str_mv | AT smli effectsofvitamind3onexpressionofdefensinstolllikereceptorsandvitamindreceptorinliverkidneyandspleenofsilkyfowl AT lhouyang effectsofvitamind3onexpressionofdefensinstolllikereceptorsandvitamindreceptorinliverkidneyandspleenofsilkyfowl AT dgzhou effectsofvitamind3onexpressionofdefensinstolllikereceptorsandvitamindreceptorinliverkidneyandspleenofsilkyfowl |