Gelatinases Increase in Bleomycin-induced Systemic Sclerosis Mouse Model
Systemic sclerosis is a fibrotic autoimmune disease in which aberrant remodeling of the extracellular matrix in organs disturbs their functionalities. The aim of this study was to investigate the expression of gelatinases on systemic sclerosis. Consequently, a mouse model of systemic sclerosis was e...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2019-04-01
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| Series: | Iranian Journal of Allergy, Asthma and Immunology |
| Subjects: | |
| Online Access: | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1956 |
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| author | Fateme Vafashoar Kazem Mousavizadeh Hadi Poormoghim Abbas Tavasoli Tahereh Musavi Shabestari Sayed Ali Javadmoosavi Nazanin Mojtabavi |
| author_facet | Fateme Vafashoar Kazem Mousavizadeh Hadi Poormoghim Abbas Tavasoli Tahereh Musavi Shabestari Sayed Ali Javadmoosavi Nazanin Mojtabavi |
| author_sort | Fateme Vafashoar |
| collection | DOAJ |
| description | Systemic sclerosis is a fibrotic autoimmune disease in which aberrant remodeling of the extracellular matrix in organs disturbs their functionalities. The aim of this study was to investigate the expression of gelatinases on systemic sclerosis. Consequently, a mouse model of systemic sclerosis was employed and the gelatinolytic activity of gelatinases was evaluated on the fibrotic tissues of this model. Two groups of ten mice were considered in this work: a group of systemic sclerosis model and control group. For the generation of systemic sclerosis model, mice received bleomycin, while the control group was subjected to phosphate buffered saline (PBS) reception. Mice were tested for fibrosis by using trichrome staining, hydroxyproline measurement and α-SMA detection in tissue sections. Additionally, the gelatinolytic activity of matrix metalloproteinase 2 and matrix metalloproteinase 9 were measured using gelatin zymography in lungs and skin tissue homogenates. The obtained results indicated that subcutaneous injection of bleomycin-induced fibrosis in skin and lung tissues of mice. Pro and active forms of matrix methaloproteinase 9 were increased in fibrotic lung tissues (p<0.05 and p<0.01, respectively), while, the gelatinolytic activity of MMP2 was unaffected in these tissues. Additionally, in skin tissues of bleomycin-treated animals, both pro and active forms of MMP9 and MMP2 were increased (p<0.05). Pro and active forms of gelatinases increase differently in skin and lung tissues of bleomycin-induced scleroderma. |
| first_indexed | 2024-12-12T22:01:23Z |
| format | Article |
| id | doaj.art-cf42370d325d4d63b76faf2d3d78b405 |
| institution | Directory Open Access Journal |
| issn | 1735-1502 1735-5249 |
| language | English |
| last_indexed | 2024-12-12T22:01:23Z |
| publishDate | 2019-04-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Allergy, Asthma and Immunology |
| spelling | doaj.art-cf42370d325d4d63b76faf2d3d78b4052022-12-22T00:10:31ZengTehran University of Medical SciencesIranian Journal of Allergy, Asthma and Immunology1735-15021735-52492019-04-0118210.18502/ijaai.v18i2.9211956Gelatinases Increase in Bleomycin-induced Systemic Sclerosis Mouse ModelFateme Vafashoar0Kazem Mousavizadeh1Hadi Poormoghim2Abbas Tavasoli3Tahereh Musavi Shabestari4Sayed Ali Javadmoosavi5Nazanin Mojtabavi6Department of Immunology, Iran University of Medical Sciences, Tehran, IranDepartment of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, IranScleroderma Study Group, Firoozgar Hospital, Iran University of Medical Sciences, Tehran, IranDepartment of Pathobiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranInstitute of Immunology and Infectious Diseases, Antimicrobial Resistance Research Center, Iran University of Medical Sciences, Tehran, IranAir Pollution Research Centre, School of Public Health, Iran University of Medical Sciences, Tehran, IranDepartment of Immunology, Iran University of Medical Sciences, Tehran, Iran AND Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, IranSystemic sclerosis is a fibrotic autoimmune disease in which aberrant remodeling of the extracellular matrix in organs disturbs their functionalities. The aim of this study was to investigate the expression of gelatinases on systemic sclerosis. Consequently, a mouse model of systemic sclerosis was employed and the gelatinolytic activity of gelatinases was evaluated on the fibrotic tissues of this model. Two groups of ten mice were considered in this work: a group of systemic sclerosis model and control group. For the generation of systemic sclerosis model, mice received bleomycin, while the control group was subjected to phosphate buffered saline (PBS) reception. Mice were tested for fibrosis by using trichrome staining, hydroxyproline measurement and α-SMA detection in tissue sections. Additionally, the gelatinolytic activity of matrix metalloproteinase 2 and matrix metalloproteinase 9 were measured using gelatin zymography in lungs and skin tissue homogenates. The obtained results indicated that subcutaneous injection of bleomycin-induced fibrosis in skin and lung tissues of mice. Pro and active forms of matrix methaloproteinase 9 were increased in fibrotic lung tissues (p<0.05 and p<0.01, respectively), while, the gelatinolytic activity of MMP2 was unaffected in these tissues. Additionally, in skin tissues of bleomycin-treated animals, both pro and active forms of MMP9 and MMP2 were increased (p<0.05). Pro and active forms of gelatinases increase differently in skin and lung tissues of bleomycin-induced scleroderma.https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1956BleomycinGelatinaseMatrix metalloproteinase 2Matrix metalloproteinase 9Systemic scleroderma |
| spellingShingle | Fateme Vafashoar Kazem Mousavizadeh Hadi Poormoghim Abbas Tavasoli Tahereh Musavi Shabestari Sayed Ali Javadmoosavi Nazanin Mojtabavi Gelatinases Increase in Bleomycin-induced Systemic Sclerosis Mouse Model Iranian Journal of Allergy, Asthma and Immunology Bleomycin Gelatinase Matrix metalloproteinase 2 Matrix metalloproteinase 9 Systemic scleroderma |
| title | Gelatinases Increase in Bleomycin-induced Systemic Sclerosis Mouse Model |
| title_full | Gelatinases Increase in Bleomycin-induced Systemic Sclerosis Mouse Model |
| title_fullStr | Gelatinases Increase in Bleomycin-induced Systemic Sclerosis Mouse Model |
| title_full_unstemmed | Gelatinases Increase in Bleomycin-induced Systemic Sclerosis Mouse Model |
| title_short | Gelatinases Increase in Bleomycin-induced Systemic Sclerosis Mouse Model |
| title_sort | gelatinases increase in bleomycin induced systemic sclerosis mouse model |
| topic | Bleomycin Gelatinase Matrix metalloproteinase 2 Matrix metalloproteinase 9 Systemic scleroderma |
| url | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1956 |
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