Effects of leptin on the viability of human ovarian cancer cells and changes in cytokine expression levels
Background Obesity is associated with increased mortality among ovarian cancer and is a poor prognostic factor. There are significant links between the leptin hormone, a product of the obesity gene, and the development of ovarian cancer. Leptin is a vital hormone-like cytokine secreted from adipose...
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PeerJ Inc.
2023-04-01
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| author | Fatih Dincer Harika Atmaca Levent Akman Latife Merve Oktay Burcak Karaca Mustafa Cosan Terek |
| author_facet | Fatih Dincer Harika Atmaca Levent Akman Latife Merve Oktay Burcak Karaca Mustafa Cosan Terek |
| author_sort | Fatih Dincer |
| collection | DOAJ |
| description | Background Obesity is associated with increased mortality among ovarian cancer and is a poor prognostic factor. There are significant links between the leptin hormone, a product of the obesity gene, and the development of ovarian cancer. Leptin is a vital hormone-like cytokine secreted from adipose tissue and is mainly involved in the maintenance of energy homeostasis. It regulates several intracellular signaling pathways and also interacts with various hormones and energy regulators. It acts as a growth factor by stimulating cell proliferation and differentiation and in this way contributes to cancer cell development. The aim of the study was to investigate the effects of leptin on human ovarian cancer cells. Methods In this study, the effects of increasing the concentration of leptin were investigated on the cell viability of OVCAR-3 and MDAH-2774 ovarian cancer lines by MTT assay. Moreover, to elucidate the molecular mechanisms of leptin in ovarian cancer cells, changes in the expression levels of 80 cytokines were evaluated after leptin treatment via a human cytokine antibody array. Results Leptin increases the proliferation of both ovarian cancer cell lines. IL-1 level was increased in OVCAR-3 cells and TGF-β level was increased in MDAH-2774 cells after leptin treatment. A decrease in IL-2, MCP-2/CCL8 and MCP-3/CCL7 levels was detected in both ovarian cancer cell lines with leptin administration. An increase in IL-3 and IL-10 expressions, insulin-like growth factor binding proteins (IGFBP) IGFBP-1, IGFBP-2 and IGFBP-3 levels were detected in both ovarian cancer cell lines with leptin administration. In conclusion; leptin has a proliferative effect on human ovarian cancer cell lines and affects different cytokines in different types of ovarian cancer cells. |
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| format | Article |
| id | doaj.art-cf43b7f3bb3f4f059c5692ca1dc1a43d |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:49:40Z |
| publishDate | 2023-04-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-cf43b7f3bb3f4f059c5692ca1dc1a43d2023-12-03T10:29:37ZengPeerJ Inc.PeerJ2167-83592023-04-0111e1524610.7717/peerj.15246Effects of leptin on the viability of human ovarian cancer cells and changes in cytokine expression levelsFatih Dincer0Harika Atmaca1Levent Akman2Latife Merve Oktay3Burcak Karaca4Mustafa Cosan Terek5Divison of Gynecologic Oncology, Health Sciences University İzmir Tepecik Education and Research Hospital, Health Sciences University, Izmir, TurkeyDepartment of Biology, Faculty of Science and Letters, Manisa Celal Bayar University, Manisa, TurkeyDepartment of Obstetrics and Gynecology, Divison of Gynecologic Oncology, Ege University, Bayraklı, İzmir, TurkeyDepartment of Medical Biology, Medicine Faculty, Ege University, Bayraklı, İzmir, TurkeyDepartment of Medical Oncology, Tulay Aktas Oncology Hospital, Ege University, Bayraklı, İzmir, TurkeyDepartment of Obstetrics and Gynecology, Divison of Gynecologic Oncology, Ege University, Bayraklı, İzmir, TurkeyBackground Obesity is associated with increased mortality among ovarian cancer and is a poor prognostic factor. There are significant links between the leptin hormone, a product of the obesity gene, and the development of ovarian cancer. Leptin is a vital hormone-like cytokine secreted from adipose tissue and is mainly involved in the maintenance of energy homeostasis. It regulates several intracellular signaling pathways and also interacts with various hormones and energy regulators. It acts as a growth factor by stimulating cell proliferation and differentiation and in this way contributes to cancer cell development. The aim of the study was to investigate the effects of leptin on human ovarian cancer cells. Methods In this study, the effects of increasing the concentration of leptin were investigated on the cell viability of OVCAR-3 and MDAH-2774 ovarian cancer lines by MTT assay. Moreover, to elucidate the molecular mechanisms of leptin in ovarian cancer cells, changes in the expression levels of 80 cytokines were evaluated after leptin treatment via a human cytokine antibody array. Results Leptin increases the proliferation of both ovarian cancer cell lines. IL-1 level was increased in OVCAR-3 cells and TGF-β level was increased in MDAH-2774 cells after leptin treatment. A decrease in IL-2, MCP-2/CCL8 and MCP-3/CCL7 levels was detected in both ovarian cancer cell lines with leptin administration. An increase in IL-3 and IL-10 expressions, insulin-like growth factor binding proteins (IGFBP) IGFBP-1, IGFBP-2 and IGFBP-3 levels were detected in both ovarian cancer cell lines with leptin administration. In conclusion; leptin has a proliferative effect on human ovarian cancer cell lines and affects different cytokines in different types of ovarian cancer cells.https://peerj.com/articles/15246.pdfObesityLeptinOvarian cancer |
| spellingShingle | Fatih Dincer Harika Atmaca Levent Akman Latife Merve Oktay Burcak Karaca Mustafa Cosan Terek Effects of leptin on the viability of human ovarian cancer cells and changes in cytokine expression levels PeerJ Obesity Leptin Ovarian cancer |
| title | Effects of leptin on the viability of human ovarian cancer cells and changes in cytokine expression levels |
| title_full | Effects of leptin on the viability of human ovarian cancer cells and changes in cytokine expression levels |
| title_fullStr | Effects of leptin on the viability of human ovarian cancer cells and changes in cytokine expression levels |
| title_full_unstemmed | Effects of leptin on the viability of human ovarian cancer cells and changes in cytokine expression levels |
| title_short | Effects of leptin on the viability of human ovarian cancer cells and changes in cytokine expression levels |
| title_sort | effects of leptin on the viability of human ovarian cancer cells and changes in cytokine expression levels |
| topic | Obesity Leptin Ovarian cancer |
| url | https://peerj.com/articles/15246.pdf |
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