Advantages, disadvantages and research progress on strategies for screening and identifying functional genes
The screening and identification of functional genes is a key step to deeply understand various biological phenotypes. Forward genetics including traditional genetics, genome-wide association analysis, the comparison of gene expression profiles and bioinformatics, and reverse genetics including cDNA...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2022-06-01
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| Series: | Jichu yixue yu linchuang |
| Subjects: | |
| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-6-969.pdf |
| _version_ | 1827386954784702464 |
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| author | LIU Xu, CHANG De, WANG Jun-feng |
| author_facet | LIU Xu, CHANG De, WANG Jun-feng |
| author_sort | LIU Xu, CHANG De, WANG Jun-feng |
| collection | DOAJ |
| description | The screening and identification of functional genes is a key step to deeply understand various biological phenotypes. Forward genetics including traditional genetics, genome-wide association analysis, the comparison of gene expression profiles and bioinformatics, and reverse genetics including cDNA library, RNA interference, CRISPR-Cas9 technology, insertion mutation as well as model organisms have been applied to screening functional genes. Different methods have their own advantages and disadvantages and technology combination to screen and to identify functional genes is a good choice of strategy. |
| first_indexed | 2024-03-08T15:54:01Z |
| format | Article |
| id | doaj.art-cf4545f17d0f4d788a6d56d995955edc |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T15:54:01Z |
| publishDate | 2022-06-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-cf4545f17d0f4d788a6d56d995955edc2024-01-09T02:51:51ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252022-06-0142696997410.16352/j.issn.1001-6325.2022.06.010Advantages, disadvantages and research progress on strategies for screening and identifying functional genesLIU Xu, CHANG De, WANG Jun-feng01. the Third Medical Center,Chinese People's Liberation Army General Hospital, Beijing 100069; ;2. the Second Medical Center,Chinese People's Liberation Army General Hospital, Beijing 100853, ChinaThe screening and identification of functional genes is a key step to deeply understand various biological phenotypes. Forward genetics including traditional genetics, genome-wide association analysis, the comparison of gene expression profiles and bioinformatics, and reverse genetics including cDNA library, RNA interference, CRISPR-Cas9 technology, insertion mutation as well as model organisms have been applied to screening functional genes. Different methods have their own advantages and disadvantages and technology combination to screen and to identify functional genes is a good choice of strategy.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-6-969.pdffunctional genes|forward genetics|reverse genetics|bioinformatics|model organism |
| spellingShingle | LIU Xu, CHANG De, WANG Jun-feng Advantages, disadvantages and research progress on strategies for screening and identifying functional genes Jichu yixue yu linchuang functional genes|forward genetics|reverse genetics|bioinformatics|model organism |
| title | Advantages, disadvantages and research progress on strategies for screening and identifying functional genes |
| title_full | Advantages, disadvantages and research progress on strategies for screening and identifying functional genes |
| title_fullStr | Advantages, disadvantages and research progress on strategies for screening and identifying functional genes |
| title_full_unstemmed | Advantages, disadvantages and research progress on strategies for screening and identifying functional genes |
| title_short | Advantages, disadvantages and research progress on strategies for screening and identifying functional genes |
| title_sort | advantages disadvantages and research progress on strategies for screening and identifying functional genes |
| topic | functional genes|forward genetics|reverse genetics|bioinformatics|model organism |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-6-969.pdf |
| work_keys_str_mv | AT liuxuchangdewangjunfeng advantagesdisadvantagesandresearchprogressonstrategiesforscreeningandidentifyingfunctionalgenes |