Effects of Deacetylasperulosidic Acid on Atopic Dermatitis through Modulating Immune Balance and Skin Barrier Function in HaCaT, HMC-1, and EOL-1 Cells
The medicinal plant noni (<i>Morinda citrifolia</i>) is widely dispersed throughout Southeast Asia, the Caribbean, and Australia. We previously reported that fermented Noni could alleviate atopic dermatitis (AD) by recovering Th1/Th2 immune balance and enhancing skin barrier function ind...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/11/3298 |
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| author | Jin Su Oh Geum Su Seong Yong Deok Kim Se Young Choung |
| author_facet | Jin Su Oh Geum Su Seong Yong Deok Kim Se Young Choung |
| author_sort | Jin Su Oh |
| collection | DOAJ |
| description | The medicinal plant noni (<i>Morinda citrifolia</i>) is widely dispersed throughout Southeast Asia, the Caribbean, and Australia. We previously reported that fermented Noni could alleviate atopic dermatitis (AD) by recovering Th1/Th2 immune balance and enhancing skin barrier function induced by 2,4-dinitrochlorobenzene. Noni has a high deacetylasperulosidic acid (DAA) content, whose concentration further increased in fermented noni as an iridoid constituent. This study aimed to determine the anti-AD effects and mechanisms of DAA on HaCaT, HMC-1, and EOL-1 cells. DAA inhibited the gene expression and secretion of AD-related cytokines and chemokines including interleukin (IL)-1β, IL-4, IL-6, IL-8, IL-25, IL-33, thymic stromal lymphopoietin, tumor necrosis factor-alpha, monocyte chemoattractant protein-1, thymus and activation-regulated chemokine, macrophage-derived chemokine, and regulated upon activation, normal T cell expressed and secreted, in all cells, and inhibited histamine release in HMC-1 cells. DAA controlled mitogen-activated protein kinase phosphorylation levels and the translocation of nuclear factor-kappa light chain enhancer of activated B cells into the nucleus by inhibiting IκBα decomposition in all the cells. Furthermore, DAA increased the expression of proteins involved in skin barrier functions such as filaggrin and involucrin in HaCaT cells. These results confirmed that DAA could relieve AD by controlling immune balance and recovering skin barrier function. |
| first_indexed | 2024-03-10T10:52:05Z |
| format | Article |
| id | doaj.art-cf4a8b562c384ab6829775e35908c834 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T10:52:05Z |
| publishDate | 2021-05-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-cf4a8b562c384ab6829775e35908c8342023-11-21T22:08:59ZengMDPI AGMolecules1420-30492021-05-012611329810.3390/molecules26113298Effects of Deacetylasperulosidic Acid on Atopic Dermatitis through Modulating Immune Balance and Skin Barrier Function in HaCaT, HMC-1, and EOL-1 CellsJin Su Oh0Geum Su Seong1Yong Deok Kim2Se Young Choung3Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, KoreaKorea Food Research Institute, 245 Nongsaengmyeong-ro, Iseo-myeon, Wanju Gun 55365, Jeollabuk-do, KoreaNST BIO Co., Ltd., Goeumdal-ro, Yangchon-eup, Gimpo-si 10049, Gyeonggi-do, KoreaDepartment of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, KoreaThe medicinal plant noni (<i>Morinda citrifolia</i>) is widely dispersed throughout Southeast Asia, the Caribbean, and Australia. We previously reported that fermented Noni could alleviate atopic dermatitis (AD) by recovering Th1/Th2 immune balance and enhancing skin barrier function induced by 2,4-dinitrochlorobenzene. Noni has a high deacetylasperulosidic acid (DAA) content, whose concentration further increased in fermented noni as an iridoid constituent. This study aimed to determine the anti-AD effects and mechanisms of DAA on HaCaT, HMC-1, and EOL-1 cells. DAA inhibited the gene expression and secretion of AD-related cytokines and chemokines including interleukin (IL)-1β, IL-4, IL-6, IL-8, IL-25, IL-33, thymic stromal lymphopoietin, tumor necrosis factor-alpha, monocyte chemoattractant protein-1, thymus and activation-regulated chemokine, macrophage-derived chemokine, and regulated upon activation, normal T cell expressed and secreted, in all cells, and inhibited histamine release in HMC-1 cells. DAA controlled mitogen-activated protein kinase phosphorylation levels and the translocation of nuclear factor-kappa light chain enhancer of activated B cells into the nucleus by inhibiting IκBα decomposition in all the cells. Furthermore, DAA increased the expression of proteins involved in skin barrier functions such as filaggrin and involucrin in HaCaT cells. These results confirmed that DAA could relieve AD by controlling immune balance and recovering skin barrier function.https://www.mdpi.com/1420-3049/26/11/3298<i>Morinda citrifolia</i>atopic dermatitisdeacetylasperulosidic acidHaCaTHMC-1EOL-1 |
| spellingShingle | Jin Su Oh Geum Su Seong Yong Deok Kim Se Young Choung Effects of Deacetylasperulosidic Acid on Atopic Dermatitis through Modulating Immune Balance and Skin Barrier Function in HaCaT, HMC-1, and EOL-1 Cells Molecules <i>Morinda citrifolia</i> atopic dermatitis deacetylasperulosidic acid HaCaT HMC-1 EOL-1 |
| title | Effects of Deacetylasperulosidic Acid on Atopic Dermatitis through Modulating Immune Balance and Skin Barrier Function in HaCaT, HMC-1, and EOL-1 Cells |
| title_full | Effects of Deacetylasperulosidic Acid on Atopic Dermatitis through Modulating Immune Balance and Skin Barrier Function in HaCaT, HMC-1, and EOL-1 Cells |
| title_fullStr | Effects of Deacetylasperulosidic Acid on Atopic Dermatitis through Modulating Immune Balance and Skin Barrier Function in HaCaT, HMC-1, and EOL-1 Cells |
| title_full_unstemmed | Effects of Deacetylasperulosidic Acid on Atopic Dermatitis through Modulating Immune Balance and Skin Barrier Function in HaCaT, HMC-1, and EOL-1 Cells |
| title_short | Effects of Deacetylasperulosidic Acid on Atopic Dermatitis through Modulating Immune Balance and Skin Barrier Function in HaCaT, HMC-1, and EOL-1 Cells |
| title_sort | effects of deacetylasperulosidic acid on atopic dermatitis through modulating immune balance and skin barrier function in hacat hmc 1 and eol 1 cells |
| topic | <i>Morinda citrifolia</i> atopic dermatitis deacetylasperulosidic acid HaCaT HMC-1 EOL-1 |
| url | https://www.mdpi.com/1420-3049/26/11/3298 |
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