A Plasmid Carrying <i>bla</i><sub>IMP-56</sub> in <i>Pseudomonas aeruginosa</i> Belonging to a Novel Resistance Plasmid Family
<i>bla</i><sub>IMP</sub> and <i>bla</i><sub>VIM</sub> are the most detected plasmid-encoded carbapenemase genes in <i>Pseudomonas aeruginosa</i>. Previous studies have reported plasmid sequences carrying <i>bla</i><sub>IMP...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/9/1863 |
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| author | Jessica Gómez-Martínez Rosa del Carmen Rocha-Gracia Elena Bello-López Miguel Angel Cevallos Miguel Castañeda-Lucio Alma López-García Yolanda Sáenz Guadalupe Jiménez-Flores Gerardo Cortés-Cortés Patricia Lozano-Zarain |
| author_facet | Jessica Gómez-Martínez Rosa del Carmen Rocha-Gracia Elena Bello-López Miguel Angel Cevallos Miguel Castañeda-Lucio Alma López-García Yolanda Sáenz Guadalupe Jiménez-Flores Gerardo Cortés-Cortés Patricia Lozano-Zarain |
| author_sort | Jessica Gómez-Martínez |
| collection | DOAJ |
| description | <i>bla</i><sub>IMP</sub> and <i>bla</i><sub>VIM</sub> are the most detected plasmid-encoded carbapenemase genes in <i>Pseudomonas aeruginosa</i>. Previous studies have reported plasmid sequences carrying <i>bla</i><sub>IMP</sub> variants, except <i>bla</i><sub>IMP-56</sub>. In this study, we aimed to characterize a plasmid carrying <i>bla</i><sub>IMP-56</sub> in a <i>P. aeruginosa</i> strain isolated from a Mexican hospital. The whole genome of <i>P. aeruginosa</i> strain PE52 was sequenced using Illumina Miseq 2 × 150 bp, with 5 million paired-end reads. We characterized a 27 kb plasmid (pPE52IMP) that carried <i>bla</i><sub>IMP-56</sub>. The phylogenetic analysis of RepA in pPE52IMP and 33 <i>P. aeruginosa</i> plasmids carrying resistance genes reported in the GenBank revealed that pPE52IMP and four plasmids (pMATVIM-7, unnamed (FDAARGOS_570), pD5170990, and pMRVIM0713) were in the same clade. These closely related plasmids belonged to the MOB<sub>P11</sub> subfamily and had similar backbones. Another plasmid (p4130-KPC) had a similar backbone to pPE52IMP; however, its RepA was truncated. In these plasmids, the resistance genes <i>bla</i><sub>KPC-2</sub>, <i>bla</i><sub>VIM</sub> variants, <i>aac(6′)-Ib4</i>, <i>bla</i><sub>OXA</sub> variants, and <i>bla</i><sub>IMP-56</sub> were inserted between <i>phd</i> and resolvase genes. This study describes a new family of plasmids carrying resistance genes, with a similar backbone, the same RepA, and belonging to the MOB<sub>P11</sub> subfamily in <i>P. aeruginosa</i>. In addition, our characterized plasmid harboring <i>bla</i><sub>IMP-56</sub> (pPE52IMP) belongs to this family. |
| first_indexed | 2024-03-09T23:05:18Z |
| format | Article |
| id | doaj.art-cf559d6d852f47be9c729fe8de0d6c4d |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T23:05:18Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-cf559d6d852f47be9c729fe8de0d6c4d2023-11-23T17:54:31ZengMDPI AGMicroorganisms2076-26072022-09-01109186310.3390/microorganisms10091863A Plasmid Carrying <i>bla</i><sub>IMP-56</sub> in <i>Pseudomonas aeruginosa</i> Belonging to a Novel Resistance Plasmid FamilyJessica Gómez-Martínez0Rosa del Carmen Rocha-Gracia1Elena Bello-López2Miguel Angel Cevallos3Miguel Castañeda-Lucio4Alma López-García5Yolanda Sáenz6Guadalupe Jiménez-Flores7Gerardo Cortés-Cortés8Patricia Lozano-Zarain9Posgrado en Microbiología, Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla 72570, MexicoPosgrado en Microbiología, Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla 72570, MexicoPrograma de Genómica Evolutiva, Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca 62210, MexicoPrograma de Genómica Evolutiva, Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca 62210, MexicoPosgrado en Microbiología, Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla 72570, MexicoDepartamento de Microbiología, Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Puebla 72570, MexicoÁrea de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja (CIBIR), 26006 Logroño, SpainLaboratorio Clínico. Área de Microbiología, Hospital Regional Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado, Puebla 72570, MexicoPosgrado en Microbiología, Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla 72570, MexicoPosgrado en Microbiología, Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla 72570, Mexico<i>bla</i><sub>IMP</sub> and <i>bla</i><sub>VIM</sub> are the most detected plasmid-encoded carbapenemase genes in <i>Pseudomonas aeruginosa</i>. Previous studies have reported plasmid sequences carrying <i>bla</i><sub>IMP</sub> variants, except <i>bla</i><sub>IMP-56</sub>. In this study, we aimed to characterize a plasmid carrying <i>bla</i><sub>IMP-56</sub> in a <i>P. aeruginosa</i> strain isolated from a Mexican hospital. The whole genome of <i>P. aeruginosa</i> strain PE52 was sequenced using Illumina Miseq 2 × 150 bp, with 5 million paired-end reads. We characterized a 27 kb plasmid (pPE52IMP) that carried <i>bla</i><sub>IMP-56</sub>. The phylogenetic analysis of RepA in pPE52IMP and 33 <i>P. aeruginosa</i> plasmids carrying resistance genes reported in the GenBank revealed that pPE52IMP and four plasmids (pMATVIM-7, unnamed (FDAARGOS_570), pD5170990, and pMRVIM0713) were in the same clade. These closely related plasmids belonged to the MOB<sub>P11</sub> subfamily and had similar backbones. Another plasmid (p4130-KPC) had a similar backbone to pPE52IMP; however, its RepA was truncated. In these plasmids, the resistance genes <i>bla</i><sub>KPC-2</sub>, <i>bla</i><sub>VIM</sub> variants, <i>aac(6′)-Ib4</i>, <i>bla</i><sub>OXA</sub> variants, and <i>bla</i><sub>IMP-56</sub> were inserted between <i>phd</i> and resolvase genes. This study describes a new family of plasmids carrying resistance genes, with a similar backbone, the same RepA, and belonging to the MOB<sub>P11</sub> subfamily in <i>P. aeruginosa</i>. In addition, our characterized plasmid harboring <i>bla</i><sub>IMP-56</sub> (pPE52IMP) belongs to this family.https://www.mdpi.com/2076-2607/10/9/1863<i>Pseudomonas aeruginosa</i>antimicrobial resistanceplasmidsblaIMP-56 |
| spellingShingle | Jessica Gómez-Martínez Rosa del Carmen Rocha-Gracia Elena Bello-López Miguel Angel Cevallos Miguel Castañeda-Lucio Alma López-García Yolanda Sáenz Guadalupe Jiménez-Flores Gerardo Cortés-Cortés Patricia Lozano-Zarain A Plasmid Carrying <i>bla</i><sub>IMP-56</sub> in <i>Pseudomonas aeruginosa</i> Belonging to a Novel Resistance Plasmid Family Microorganisms <i>Pseudomonas aeruginosa</i> antimicrobial resistance plasmids blaIMP-56 |
| title | A Plasmid Carrying <i>bla</i><sub>IMP-56</sub> in <i>Pseudomonas aeruginosa</i> Belonging to a Novel Resistance Plasmid Family |
| title_full | A Plasmid Carrying <i>bla</i><sub>IMP-56</sub> in <i>Pseudomonas aeruginosa</i> Belonging to a Novel Resistance Plasmid Family |
| title_fullStr | A Plasmid Carrying <i>bla</i><sub>IMP-56</sub> in <i>Pseudomonas aeruginosa</i> Belonging to a Novel Resistance Plasmid Family |
| title_full_unstemmed | A Plasmid Carrying <i>bla</i><sub>IMP-56</sub> in <i>Pseudomonas aeruginosa</i> Belonging to a Novel Resistance Plasmid Family |
| title_short | A Plasmid Carrying <i>bla</i><sub>IMP-56</sub> in <i>Pseudomonas aeruginosa</i> Belonging to a Novel Resistance Plasmid Family |
| title_sort | plasmid carrying i bla i sub imp 56 sub in i pseudomonas aeruginosa i belonging to a novel resistance plasmid family |
| topic | <i>Pseudomonas aeruginosa</i> antimicrobial resistance plasmids blaIMP-56 |
| url | https://www.mdpi.com/2076-2607/10/9/1863 |
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