A genome-wide study of DNA methylation in white blood cells and asthma in Latino children and youth
Latinos are heavily affected with childhood asthma. Little is known about epigenetic mechanisms of asthma in Latino youth. We conducted a meta-analysis of two epigenome-wide association studies (EWAS) of asthma, using DNA from white blood cells (WBCs) from 1,136 Latino children and youth aged 6 to 2...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-05-01
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| Series: | Epigenetics |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592294.2020.1809872 |
| _version_ | 1827811499649794048 |
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| author | Yale Jiang Erick Forno Yueh-Ying Han Zhongli Xu Donglei Hu Nadia Boutaoui Celeste Eng Edna Acosta-Pérez Scott Huntsman Angel Colón-Semidey Kevin L. Keys José R. Rodríguez-Santana María Alvarez Maria Pino-Yanes Glorisa Canino Wei Chen Esteban G. Burchard Juan C. Celedón |
| author_facet | Yale Jiang Erick Forno Yueh-Ying Han Zhongli Xu Donglei Hu Nadia Boutaoui Celeste Eng Edna Acosta-Pérez Scott Huntsman Angel Colón-Semidey Kevin L. Keys José R. Rodríguez-Santana María Alvarez Maria Pino-Yanes Glorisa Canino Wei Chen Esteban G. Burchard Juan C. Celedón |
| author_sort | Yale Jiang |
| collection | DOAJ |
| description | Latinos are heavily affected with childhood asthma. Little is known about epigenetic mechanisms of asthma in Latino youth. We conducted a meta-analysis of two epigenome-wide association studies (EWAS) of asthma, using DNA from white blood cells (WBCs) from 1,136 Latino children and youth aged 6 to 20 years. Genes near the top CpG sites in this EWAS were examined in a pathway enrichment analysis, and we then assessed whether our results replicated those from publicly available data from three independent EWAS conducted in non-Latino populations. We found that DNA methylation profiles differed between subjects with and without asthma. After adjustment for covariates and multiple testing, two CpGs were differentially methylated at a false discovery rate (FDR)-adjusted P < 0.1, and 193 CpG sites were differentially methylated at FDR-adjusted P < 0.2. The two top CpGs are near genes relevant to inflammatory signalling, including CAMK1D (Calcium/Calmodulin Dependent Protein Kinase ID) and TIGIT (T Cell Immunoreceptor With Ig And ITIM Domains). Moreover, 25 genomic regions were differentially methylated between subjects with and without asthma, at Šidák-corrected P < 0.10. An enrichment analysis then identified the TGF-beta pathway as most relevant to asthma in our analysis, and we replicated some of the top signals from publicly available EWAS datasets in non-Hispanic populations. In conclusion, we have identified novel epigenetic markers of asthma in WBCs from Latino children and youth, while also replicating previous results from studies conducted in non-Latinos. |
| first_indexed | 2024-03-11T23:05:54Z |
| format | Article |
| id | doaj.art-cf590e9b7397474d90b1a02e04c96e6c |
| institution | Directory Open Access Journal |
| issn | 1559-2294 1559-2308 |
| language | English |
| last_indexed | 2024-03-11T23:05:54Z |
| publishDate | 2021-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Epigenetics |
| spelling | doaj.art-cf590e9b7397474d90b1a02e04c96e6c2023-09-21T13:09:24ZengTaylor & Francis GroupEpigenetics1559-22941559-23082021-05-0116557758510.1080/15592294.2020.18098721809872A genome-wide study of DNA methylation in white blood cells and asthma in Latino children and youthYale Jiang0Erick Forno1Yueh-Ying Han2Zhongli Xu3Donglei Hu4Nadia Boutaoui5Celeste Eng6Edna Acosta-Pérez7Scott Huntsman8Angel Colón-Semidey9Kevin L. Keys10José R. Rodríguez-Santana11María Alvarez12Maria Pino-Yanes13Glorisa Canino14Wei Chen15Esteban G. Burchard16Juan C. Celedón17UPMC Children’s Hospital of PittsburghUPMC Children’s Hospital of PittsburghUPMC Children’s Hospital of PittsburghUPMC Children’s Hospital of PittsburghUniversity of California San FranciscoUPMC Children’s Hospital of PittsburghUniversity of California San FranciscoBehavioral Sciences Research Institute, Medical Science Campus, University of Puerto RicoUniversity of California San FranciscoUniversity of Puerto RicoUniversity of California San FranciscoCentro de Neumología PediátricaUniversity of Puerto RicoUniversidad de La LagunaBehavioral Sciences Research Institute, Medical Science Campus, University of Puerto RicoUPMC Children’s Hospital of PittsburghUniversity of California San FranciscoUPMC Children’s Hospital of PittsburghLatinos are heavily affected with childhood asthma. Little is known about epigenetic mechanisms of asthma in Latino youth. We conducted a meta-analysis of two epigenome-wide association studies (EWAS) of asthma, using DNA from white blood cells (WBCs) from 1,136 Latino children and youth aged 6 to 20 years. Genes near the top CpG sites in this EWAS were examined in a pathway enrichment analysis, and we then assessed whether our results replicated those from publicly available data from three independent EWAS conducted in non-Latino populations. We found that DNA methylation profiles differed between subjects with and without asthma. After adjustment for covariates and multiple testing, two CpGs were differentially methylated at a false discovery rate (FDR)-adjusted P < 0.1, and 193 CpG sites were differentially methylated at FDR-adjusted P < 0.2. The two top CpGs are near genes relevant to inflammatory signalling, including CAMK1D (Calcium/Calmodulin Dependent Protein Kinase ID) and TIGIT (T Cell Immunoreceptor With Ig And ITIM Domains). Moreover, 25 genomic regions were differentially methylated between subjects with and without asthma, at Šidák-corrected P < 0.10. An enrichment analysis then identified the TGF-beta pathway as most relevant to asthma in our analysis, and we replicated some of the top signals from publicly available EWAS datasets in non-Hispanic populations. In conclusion, we have identified novel epigenetic markers of asthma in WBCs from Latino children and youth, while also replicating previous results from studies conducted in non-Latinos.http://dx.doi.org/10.1080/15592294.2020.1809872asthmaepigenome-widedna methylationwhite blood cells |
| spellingShingle | Yale Jiang Erick Forno Yueh-Ying Han Zhongli Xu Donglei Hu Nadia Boutaoui Celeste Eng Edna Acosta-Pérez Scott Huntsman Angel Colón-Semidey Kevin L. Keys José R. Rodríguez-Santana María Alvarez Maria Pino-Yanes Glorisa Canino Wei Chen Esteban G. Burchard Juan C. Celedón A genome-wide study of DNA methylation in white blood cells and asthma in Latino children and youth Epigenetics asthma epigenome-wide dna methylation white blood cells |
| title | A genome-wide study of DNA methylation in white blood cells and asthma in Latino children and youth |
| title_full | A genome-wide study of DNA methylation in white blood cells and asthma in Latino children and youth |
| title_fullStr | A genome-wide study of DNA methylation in white blood cells and asthma in Latino children and youth |
| title_full_unstemmed | A genome-wide study of DNA methylation in white blood cells and asthma in Latino children and youth |
| title_short | A genome-wide study of DNA methylation in white blood cells and asthma in Latino children and youth |
| title_sort | genome wide study of dna methylation in white blood cells and asthma in latino children and youth |
| topic | asthma epigenome-wide dna methylation white blood cells |
| url | http://dx.doi.org/10.1080/15592294.2020.1809872 |
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