COLEC12 Promotes Tumor Progression and Is Correlated With Poor Prognosis in Gastric Cancer
Purpose Collectin subfamily member 12, a transmembrane scavenger receptor C-type lectin, is aberrantly expressed in various cancers. However, its physiological role in gastric cancer remains somewhat unclear. This study aimed to investigate the Collectin subfamily member 12 expression pattern in hum...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2023-12-01
|
| Series: | Technology in Cancer Research & Treatment |
| Online Access: | https://doi.org/10.1177/15330338231218163 |
| _version_ | 1797384637703520256 |
|---|---|
| author | Xiangfei Sun PhD Qiang Zhang PhD Ping Shu PhD Xiaohan Lin PhD Xiaodong Gao PhD Kuntang Shen PhD |
| author_facet | Xiangfei Sun PhD Qiang Zhang PhD Ping Shu PhD Xiaohan Lin PhD Xiaodong Gao PhD Kuntang Shen PhD |
| author_sort | Xiangfei Sun PhD |
| collection | DOAJ |
| description | Purpose Collectin subfamily member 12, a transmembrane scavenger receptor C-type lectin, is aberrantly expressed in various cancers. However, its physiological role in gastric cancer remains somewhat unclear. This study aimed to investigate the Collectin subfamily member 12 expression pattern in human gastric cancer and its role in gastric cancer progression. Methods The Kaplan-Meier method was used for survival analysis. The univariate and multivariate Cox proportional hazards regression models were used to identify independent predictors for progression-free survival and overall survival. The effects of Collectin subfamily member 12 on gastric cancer cell proliferation, migration, invasion, and apoptosis were detected through the cell counting kit-8 assay, colony formation assay, wound healing assay, transwell assay, and flow cytometry analysis, respectively. Additionally, the correlation between Collectin subfamily member 12 expression and immune cell infiltration was analyzed through bioinformatics. Results Collectin subfamily member 12 was highly expressed in advanced gastric cancer (T3-T4, pathologic stage III-IV). High Collectin subfamily member 12 expression was correlated with a worse progression-free survival and overall survival in the gastric cancer patients. In vitro , cell line studies revealed that Collectin subfamily member 12 promoted gastric cancer cell proliferation, migration, and invasion and inhibited gastric cancer cell apoptosis. The bioinformatics analysis further demonstrated that the Collectin subfamily member 12 expression level positively correlated with infiltration of several immune cells, such as M2 macrophages, dendritic cells, neutrophils, and regulatory T cells, suggesting that Collectin subfamily member 12 may also play a role in suppressing tumor immune response in gastric cancer. Conclusions Collectin subfamily member 12 was identified as a novel predictive marker and target for the clinical treatment of gastric cancer. |
| first_indexed | 2024-03-08T21:39:01Z |
| format | Article |
| id | doaj.art-cf606987702e4360beba01b1ac7c941d |
| institution | Directory Open Access Journal |
| issn | 1533-0338 |
| language | English |
| last_indexed | 2024-03-08T21:39:01Z |
| publishDate | 2023-12-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Technology in Cancer Research & Treatment |
| spelling | doaj.art-cf606987702e4360beba01b1ac7c941d2023-12-20T16:05:16ZengSAGE PublishingTechnology in Cancer Research & Treatment1533-03382023-12-012210.1177/15330338231218163COLEC12 Promotes Tumor Progression and Is Correlated With Poor Prognosis in Gastric CancerXiangfei Sun PhDQiang Zhang PhDPing Shu PhDXiaohan Lin PhDXiaodong Gao PhDKuntang Shen PhDPurpose Collectin subfamily member 12, a transmembrane scavenger receptor C-type lectin, is aberrantly expressed in various cancers. However, its physiological role in gastric cancer remains somewhat unclear. This study aimed to investigate the Collectin subfamily member 12 expression pattern in human gastric cancer and its role in gastric cancer progression. Methods The Kaplan-Meier method was used for survival analysis. The univariate and multivariate Cox proportional hazards regression models were used to identify independent predictors for progression-free survival and overall survival. The effects of Collectin subfamily member 12 on gastric cancer cell proliferation, migration, invasion, and apoptosis were detected through the cell counting kit-8 assay, colony formation assay, wound healing assay, transwell assay, and flow cytometry analysis, respectively. Additionally, the correlation between Collectin subfamily member 12 expression and immune cell infiltration was analyzed through bioinformatics. Results Collectin subfamily member 12 was highly expressed in advanced gastric cancer (T3-T4, pathologic stage III-IV). High Collectin subfamily member 12 expression was correlated with a worse progression-free survival and overall survival in the gastric cancer patients. In vitro , cell line studies revealed that Collectin subfamily member 12 promoted gastric cancer cell proliferation, migration, and invasion and inhibited gastric cancer cell apoptosis. The bioinformatics analysis further demonstrated that the Collectin subfamily member 12 expression level positively correlated with infiltration of several immune cells, such as M2 macrophages, dendritic cells, neutrophils, and regulatory T cells, suggesting that Collectin subfamily member 12 may also play a role in suppressing tumor immune response in gastric cancer. Conclusions Collectin subfamily member 12 was identified as a novel predictive marker and target for the clinical treatment of gastric cancer.https://doi.org/10.1177/15330338231218163 |
| spellingShingle | Xiangfei Sun PhD Qiang Zhang PhD Ping Shu PhD Xiaohan Lin PhD Xiaodong Gao PhD Kuntang Shen PhD COLEC12 Promotes Tumor Progression and Is Correlated With Poor Prognosis in Gastric Cancer Technology in Cancer Research & Treatment |
| title | COLEC12 Promotes Tumor Progression and Is Correlated With Poor Prognosis in Gastric Cancer |
| title_full | COLEC12 Promotes Tumor Progression and Is Correlated With Poor Prognosis in Gastric Cancer |
| title_fullStr | COLEC12 Promotes Tumor Progression and Is Correlated With Poor Prognosis in Gastric Cancer |
| title_full_unstemmed | COLEC12 Promotes Tumor Progression and Is Correlated With Poor Prognosis in Gastric Cancer |
| title_short | COLEC12 Promotes Tumor Progression and Is Correlated With Poor Prognosis in Gastric Cancer |
| title_sort | colec12 promotes tumor progression and is correlated with poor prognosis in gastric cancer |
| url | https://doi.org/10.1177/15330338231218163 |
| work_keys_str_mv | AT xiangfeisunphd colec12promotestumorprogressionandiscorrelatedwithpoorprognosisingastriccancer AT qiangzhangphd colec12promotestumorprogressionandiscorrelatedwithpoorprognosisingastriccancer AT pingshuphd colec12promotestumorprogressionandiscorrelatedwithpoorprognosisingastriccancer AT xiaohanlinphd colec12promotestumorprogressionandiscorrelatedwithpoorprognosisingastriccancer AT xiaodonggaophd colec12promotestumorprogressionandiscorrelatedwithpoorprognosisingastriccancer AT kuntangshenphd colec12promotestumorprogressionandiscorrelatedwithpoorprognosisingastriccancer |