Mutational probing of protein aggregates to design aggregation‐resistant proteins

Mutational probing of protein aggregates to design aggregation‐resistant proteins

Characterization of amorphous protein aggregates may offer insights into the process of aggregation. Eleven single amino acid mutants of lipase (LipA of Bacillus subtilis) were subjected to temperature‐induced aggregation, and the resultant aggregates were characterized for recovery of activity in t...

Full description

Bibliographic Details
Main Authors: Mohamad Zahid Kamal, Virender Kumar, Kundarapu Satyamurthi, Kushal Kumar Das, Nalam Madhusudhana Rao
Format: Article
Language:English
Published: Wiley 2016-02-01
Series:FEBS Open Bio
Subjects:
aggregation resistance
intermediates
local unfolding
mutations
protein stability
Online Access:https://doi.org/10.1002/2211-5463.12003

Similar Items

Similar Items