Characterizations of Anti-Alpha-Fetoprotein-Conjugated Magnetic Nanoparticles Associated with Alpha-Fetoprotein for Biomedical Applications
In this work, we report characterizations of biofunctionalized magnetic nanoparticles (BMNPs) associated with alpha-fetoprotein (AFP) for biomedical applications. The example BMNP in this study is anti-alpha-fetoprotein (anti-AFP) conjugated onto dextran-coated Fe3O4 labeled as Fe3O4-anti-AFP, and t...
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| Format: | Article |
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MDPI AG
2017-09-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/17/9/2018 |
| _version_ | 1828114862653308928 |
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| author | Shu-Hsien Liao Han-Sheng Huang Jen-Jie Chieh Yu-Kai Su Yuan-Fu Tong Kai-Wen Huang |
| author_facet | Shu-Hsien Liao Han-Sheng Huang Jen-Jie Chieh Yu-Kai Su Yuan-Fu Tong Kai-Wen Huang |
| author_sort | Shu-Hsien Liao |
| collection | DOAJ |
| description | In this work, we report characterizations of biofunctionalized magnetic nanoparticles (BMNPs) associated with alpha-fetoprotein (AFP) for biomedical applications. The example BMNP in this study is anti-alpha-fetoprotein (anti-AFP) conjugated onto dextran-coated Fe3O4 labeled as Fe3O4-anti-AFP, and the target is AFP. We characterize magnetic properties, such as increments of magnetization ΔMH and effective relaxation time Δτeff in the reaction process. It is found that both ΔMH and Δτeff are enhanced when the concentration of AFP, ФAFP, increases. The enhancements are due to magnetic interactions among BMNPs in magnetic clusters, which contribute extra MH after the association with MH and in turn enhance τeff. The screening of patients carrying hepatocellular carcinoma (HCC) is verified via ΔMH/MH. The proposed method can be applied to detect a wide variety of analytes. The scaling characteristics of ΔMH/MH show the potential to develop a vibrating sample magnetometer system with low field strength for clinic applications. |
| first_indexed | 2024-04-11T12:32:43Z |
| format | Article |
| id | doaj.art-cf636182285e46578c2b9cb5abe19289 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T12:32:43Z |
| publishDate | 2017-09-01 |
| publisher | MDPI AG |
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| series | Sensors |
| spelling | doaj.art-cf636182285e46578c2b9cb5abe192892022-12-22T04:23:43ZengMDPI AGSensors1424-82202017-09-01179201810.3390/s17092018s17092018Characterizations of Anti-Alpha-Fetoprotein-Conjugated Magnetic Nanoparticles Associated with Alpha-Fetoprotein for Biomedical ApplicationsShu-Hsien Liao0Han-Sheng Huang1Jen-Jie Chieh2Yu-Kai Su3Yuan-Fu Tong4Kai-Wen Huang5Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, TaiwanInstitute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, TaiwanInstitute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, TaiwanInstitute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, TaiwanInstitute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, TaiwanDepartment of Surgery and Hepatitis Research Center, National Taiwan University Hospital, Taipei 100, TaiwanIn this work, we report characterizations of biofunctionalized magnetic nanoparticles (BMNPs) associated with alpha-fetoprotein (AFP) for biomedical applications. The example BMNP in this study is anti-alpha-fetoprotein (anti-AFP) conjugated onto dextran-coated Fe3O4 labeled as Fe3O4-anti-AFP, and the target is AFP. We characterize magnetic properties, such as increments of magnetization ΔMH and effective relaxation time Δτeff in the reaction process. It is found that both ΔMH and Δτeff are enhanced when the concentration of AFP, ФAFP, increases. The enhancements are due to magnetic interactions among BMNPs in magnetic clusters, which contribute extra MH after the association with MH and in turn enhance τeff. The screening of patients carrying hepatocellular carcinoma (HCC) is verified via ΔMH/MH. The proposed method can be applied to detect a wide variety of analytes. The scaling characteristics of ΔMH/MH show the potential to develop a vibrating sample magnetometer system with low field strength for clinic applications.https://www.mdpi.com/1424-8220/17/9/2018magnetic immunoassaybiofunctionalized magnetic nanoparticlesbiomarkeralpha-fetoproteinhepatocellular carcinomamagnetization enhancement |
| spellingShingle | Shu-Hsien Liao Han-Sheng Huang Jen-Jie Chieh Yu-Kai Su Yuan-Fu Tong Kai-Wen Huang Characterizations of Anti-Alpha-Fetoprotein-Conjugated Magnetic Nanoparticles Associated with Alpha-Fetoprotein for Biomedical Applications Sensors magnetic immunoassay biofunctionalized magnetic nanoparticles biomarker alpha-fetoprotein hepatocellular carcinoma magnetization enhancement |
| title | Characterizations of Anti-Alpha-Fetoprotein-Conjugated Magnetic Nanoparticles Associated with Alpha-Fetoprotein for Biomedical Applications |
| title_full | Characterizations of Anti-Alpha-Fetoprotein-Conjugated Magnetic Nanoparticles Associated with Alpha-Fetoprotein for Biomedical Applications |
| title_fullStr | Characterizations of Anti-Alpha-Fetoprotein-Conjugated Magnetic Nanoparticles Associated with Alpha-Fetoprotein for Biomedical Applications |
| title_full_unstemmed | Characterizations of Anti-Alpha-Fetoprotein-Conjugated Magnetic Nanoparticles Associated with Alpha-Fetoprotein for Biomedical Applications |
| title_short | Characterizations of Anti-Alpha-Fetoprotein-Conjugated Magnetic Nanoparticles Associated with Alpha-Fetoprotein for Biomedical Applications |
| title_sort | characterizations of anti alpha fetoprotein conjugated magnetic nanoparticles associated with alpha fetoprotein for biomedical applications |
| topic | magnetic immunoassay biofunctionalized magnetic nanoparticles biomarker alpha-fetoprotein hepatocellular carcinoma magnetization enhancement |
| url | https://www.mdpi.com/1424-8220/17/9/2018 |
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