Use of DMACA to visualise flavan-3-ols in grape berry skins (<em>Vitis vinifera</em> L., CV Cabernet Franc)

Phenolic compounds are of great importance in Enology. They play a major role in the elaboration of red wines and during wine preservation, and contribute greatly to their organoleptic properties. Among them, flavonoïds constitute the major group present in berry skins. Their histological study is b...

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Main Authors: Michel Chevalier, Emilie Perrochon, Marie Tellier, Gérard Barbeau
Format: Article
Language:English
Published: International Viticulture and Enology Society 2003-09-01
Series:OENO One
Subjects:
Online Access:https://oeno-one.eu/article/view/949
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author Michel Chevalier
Emilie Perrochon
Marie Tellier
Gérard Barbeau
author_facet Michel Chevalier
Emilie Perrochon
Marie Tellier
Gérard Barbeau
author_sort Michel Chevalier
collection DOAJ
description Phenolic compounds are of great importance in Enology. They play a major role in the elaboration of red wines and during wine preservation, and contribute greatly to their organoleptic properties. Among them, flavonoïds constitute the major group present in berry skins. Their histological study is based classically on the use of Toluidine blue O as a staining agent, which is not specific of any type of phenolics. The use of DMACA (p-dimethylaminocinnamaldehyde), which is specific of tannins (flavan-3-ols), permitted to precise the localisation of these compounds without any risk of error ; it allowed also for the visualisation of tannins, stuck to the tonoplast, which had not been identified with Toluidine blue O.
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spelling doaj.art-cf66a71e946c41dd9ee7b630b784f31a2022-12-21T20:24:56ZengInternational Viticulture and Enology SocietyOENO One2494-12712003-09-0137319519810.20870/oeno-one.2003.37.3.949949Use of DMACA to visualise flavan-3-ols in grape berry skins (<em>Vitis vinifera</em> L., CV Cabernet Franc)Michel Chevalier0Emilie Perrochon1Marie Tellier2Gérard Barbeau3UMR 1259, Génétique et Horticulture (GenHort), INRA Angers-Nantes, 42 rue Georges Morel, BP 60057, 49071 Beaucouzé Cedex, FranceINRA, Unité expérimentale Vigne et Vin, 49071 Beaucouzé cedex, FranceINRA, Unité d'amélioration des espèces fruitières et ornementales, 49071 Beaucouzé cedex, FranceINRA, UE1117 UVV, F-49071 Angers, FrancePhenolic compounds are of great importance in Enology. They play a major role in the elaboration of red wines and during wine preservation, and contribute greatly to their organoleptic properties. Among them, flavonoïds constitute the major group present in berry skins. Their histological study is based classically on the use of Toluidine blue O as a staining agent, which is not specific of any type of phenolics. The use of DMACA (p-dimethylaminocinnamaldehyde), which is specific of tannins (flavan-3-ols), permitted to precise the localisation of these compounds without any risk of error ; it allowed also for the visualisation of tannins, stuck to the tonoplast, which had not been identified with Toluidine blue O.https://oeno-one.eu/article/view/949grapevinephenolicsstaining procedurep-dimethylaminocinnamaldehydeToluidine blue O
spellingShingle Michel Chevalier
Emilie Perrochon
Marie Tellier
Gérard Barbeau
Use of DMACA to visualise flavan-3-ols in grape berry skins (<em>Vitis vinifera</em> L., CV Cabernet Franc)
OENO One
grapevine
phenolics
staining procedure
p-dimethylaminocinnamaldehyde
Toluidine blue O
title Use of DMACA to visualise flavan-3-ols in grape berry skins (<em>Vitis vinifera</em> L., CV Cabernet Franc)
title_full Use of DMACA to visualise flavan-3-ols in grape berry skins (<em>Vitis vinifera</em> L., CV Cabernet Franc)
title_fullStr Use of DMACA to visualise flavan-3-ols in grape berry skins (<em>Vitis vinifera</em> L., CV Cabernet Franc)
title_full_unstemmed Use of DMACA to visualise flavan-3-ols in grape berry skins (<em>Vitis vinifera</em> L., CV Cabernet Franc)
title_short Use of DMACA to visualise flavan-3-ols in grape berry skins (<em>Vitis vinifera</em> L., CV Cabernet Franc)
title_sort use of dmaca to visualise flavan 3 ols in grape berry skins em vitis vinifera em l cv cabernet franc
topic grapevine
phenolics
staining procedure
p-dimethylaminocinnamaldehyde
Toluidine blue O
url https://oeno-one.eu/article/view/949
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