Development of a nucleic acid-based lateral flow device as a reliable diagnostic tool for respiratory viral infections
Viral infections continue to pose a significant threat to the public health, leading to high morbidity and mortality rates worldwide. To combat these challenges, early detection and treatment are essential in reducing hospitalizations and preventing severe complications. Simple, inexpensive, and sen...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | MethodsX |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016123003680 |
| _version_ | 1797405465589579776 |
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| author | Pınar Akalın Ayten Yazgan-Karataş |
| author_facet | Pınar Akalın Ayten Yazgan-Karataş |
| author_sort | Pınar Akalın |
| collection | DOAJ |
| description | Viral infections continue to pose a significant threat to the public health, leading to high morbidity and mortality rates worldwide. To combat these challenges, early detection and treatment are essential in reducing hospitalizations and preventing severe complications. Simple, inexpensive, and sensitive diagnostic methods are in constant demand in many areas. In this study, we report the development of a nucleic acid-based lateral flow immunoassay device (NALFIA) and demonstrate its successful application in conjunction with a multiplexed reverse-transcription loop-mediated isothermal amplification assay (LAMP) for the detection of SARS-CoV-2 and influenza. In our approach the NALFIA part preparation is independent of the target, and has the potential to ensure widespread use in diagnostics particularly where testing speed is critical such as in respiratory viral infections. • Simple, inexpensive, sensitive and reliable rapid diagnostic tool. • Target independent design. • Effective use for respiratory samples due to practical sample extraction. |
| first_indexed | 2024-03-09T03:10:18Z |
| format | Article |
| id | doaj.art-cf66cfa61dc04748995b37881491b71b |
| institution | Directory Open Access Journal |
| issn | 2215-0161 |
| language | English |
| last_indexed | 2024-03-09T03:10:18Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj.art-cf66cfa61dc04748995b37881491b71b2023-12-04T05:22:29ZengElsevierMethodsX2215-01612023-12-0111102372Development of a nucleic acid-based lateral flow device as a reliable diagnostic tool for respiratory viral infectionsPınar Akalın0Ayten Yazgan-Karataş1Dr. Orhan Öcalgiray Molecular Biology, Biotechnology and Genetics Research Center (ITU-MOBGAM), Istanbul Technical University, Maslak 34469, Istanbul, Turkey; Corresponding author.Dr. Orhan Öcalgiray Molecular Biology, Biotechnology and Genetics Research Center (ITU-MOBGAM), Istanbul Technical University, Maslak 34469, Istanbul, Turkey; Faculty of Science and Letters, Department of Molecular Biology and Genetics, Istanbul Technical University, Maslak 34469 Istanbul, TurkeyViral infections continue to pose a significant threat to the public health, leading to high morbidity and mortality rates worldwide. To combat these challenges, early detection and treatment are essential in reducing hospitalizations and preventing severe complications. Simple, inexpensive, and sensitive diagnostic methods are in constant demand in many areas. In this study, we report the development of a nucleic acid-based lateral flow immunoassay device (NALFIA) and demonstrate its successful application in conjunction with a multiplexed reverse-transcription loop-mediated isothermal amplification assay (LAMP) for the detection of SARS-CoV-2 and influenza. In our approach the NALFIA part preparation is independent of the target, and has the potential to ensure widespread use in diagnostics particularly where testing speed is critical such as in respiratory viral infections. • Simple, inexpensive, sensitive and reliable rapid diagnostic tool. • Target independent design. • Effective use for respiratory samples due to practical sample extraction.http://www.sciencedirect.com/science/article/pii/S2215016123003680Universal-NALFIA U-NALF |
| spellingShingle | Pınar Akalın Ayten Yazgan-Karataş Development of a nucleic acid-based lateral flow device as a reliable diagnostic tool for respiratory viral infections MethodsX Universal-NALFIA U-NALF |
| title | Development of a nucleic acid-based lateral flow device as a reliable diagnostic tool for respiratory viral infections |
| title_full | Development of a nucleic acid-based lateral flow device as a reliable diagnostic tool for respiratory viral infections |
| title_fullStr | Development of a nucleic acid-based lateral flow device as a reliable diagnostic tool for respiratory viral infections |
| title_full_unstemmed | Development of a nucleic acid-based lateral flow device as a reliable diagnostic tool for respiratory viral infections |
| title_short | Development of a nucleic acid-based lateral flow device as a reliable diagnostic tool for respiratory viral infections |
| title_sort | development of a nucleic acid based lateral flow device as a reliable diagnostic tool for respiratory viral infections |
| topic | Universal-NALFIA U-NALF |
| url | http://www.sciencedirect.com/science/article/pii/S2215016123003680 |
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