Trypan blue dye enters viable cells incubated with the pore-forming toxin HlyII of Bacillus cereus.
Trypan blue is a dye that has been widely used for selective staining of dead tissues or cells. Here, we show that the pore-forming toxin HlyII of Bacillus cereus allows trypan blue staining of macrophage cells, despite the cells remaining viable and metabolically active. These findings suggest that...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3167804?pdf=render |
| _version_ | 1819266624230785024 |
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| author | Seav-Ly Tran Andrea Puhar Maud Ngo-Camus Nalini Ramarao |
| author_facet | Seav-Ly Tran Andrea Puhar Maud Ngo-Camus Nalini Ramarao |
| author_sort | Seav-Ly Tran |
| collection | DOAJ |
| description | Trypan blue is a dye that has been widely used for selective staining of dead tissues or cells. Here, we show that the pore-forming toxin HlyII of Bacillus cereus allows trypan blue staining of macrophage cells, despite the cells remaining viable and metabolically active. These findings suggest that the dye enters viable cells through the pores. To our knowledge, this is the first demonstration that trypan blue may enter viable cells. Consequently, the use of trypan blue staining as a marker of vital status should be interpreted with caution. The blue coloration does not necessarily indicate cell lysis, but may rather indicate pore formation in the cell membranes and more generally increased membrane permeability. |
| first_indexed | 2024-12-23T21:04:14Z |
| format | Article |
| id | doaj.art-cf69a6572a0f4e9393101e57fc3f84e2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T21:04:14Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-cf69a6572a0f4e9393101e57fc3f84e22022-12-21T17:31:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0169e2287610.1371/journal.pone.0022876Trypan blue dye enters viable cells incubated with the pore-forming toxin HlyII of Bacillus cereus.Seav-Ly TranAndrea PuharMaud Ngo-CamusNalini RamaraoTrypan blue is a dye that has been widely used for selective staining of dead tissues or cells. Here, we show that the pore-forming toxin HlyII of Bacillus cereus allows trypan blue staining of macrophage cells, despite the cells remaining viable and metabolically active. These findings suggest that the dye enters viable cells through the pores. To our knowledge, this is the first demonstration that trypan blue may enter viable cells. Consequently, the use of trypan blue staining as a marker of vital status should be interpreted with caution. The blue coloration does not necessarily indicate cell lysis, but may rather indicate pore formation in the cell membranes and more generally increased membrane permeability.http://europepmc.org/articles/PMC3167804?pdf=render |
| spellingShingle | Seav-Ly Tran Andrea Puhar Maud Ngo-Camus Nalini Ramarao Trypan blue dye enters viable cells incubated with the pore-forming toxin HlyII of Bacillus cereus. PLoS ONE |
| title | Trypan blue dye enters viable cells incubated with the pore-forming toxin HlyII of Bacillus cereus. |
| title_full | Trypan blue dye enters viable cells incubated with the pore-forming toxin HlyII of Bacillus cereus. |
| title_fullStr | Trypan blue dye enters viable cells incubated with the pore-forming toxin HlyII of Bacillus cereus. |
| title_full_unstemmed | Trypan blue dye enters viable cells incubated with the pore-forming toxin HlyII of Bacillus cereus. |
| title_short | Trypan blue dye enters viable cells incubated with the pore-forming toxin HlyII of Bacillus cereus. |
| title_sort | trypan blue dye enters viable cells incubated with the pore forming toxin hlyii of bacillus cereus |
| url | http://europepmc.org/articles/PMC3167804?pdf=render |
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