Shibi Tea (<i>Adinandra nitida</i>) and Camellianin A Alleviate CCl<sub>4</sub>-Induced Liver Injury in C57BL-6J Mice by Attenuation of Oxidative Stress, Inflammation, and Apoptosis
Liver injury is a significant public health issue nowadays. Shibi tea is a non-<i>Camellia</i> tea prepared from the dried leaves of <i>Adinandra nitida</i>, one of the plants with the greatest flavonoid concentration, with Camellianin A (CA) being the major flavonoid. Shibi...
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MDPI AG
2022-07-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/15/3037 |
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| author | Ruohong Chen Yingyi Lian Shuai Wen Qiuhua Li Lingli Sun Xingfei Lai Zhenbiao Zhang Junquan Zhu Linsong Tang Ji Xuan Erdong Yuan Shili Sun |
| author_facet | Ruohong Chen Yingyi Lian Shuai Wen Qiuhua Li Lingli Sun Xingfei Lai Zhenbiao Zhang Junquan Zhu Linsong Tang Ji Xuan Erdong Yuan Shili Sun |
| author_sort | Ruohong Chen |
| collection | DOAJ |
| description | Liver injury is a significant public health issue nowadays. Shibi tea is a non-<i>Camellia</i> tea prepared from the dried leaves of <i>Adinandra nitida</i>, one of the plants with the greatest flavonoid concentration, with Camellianin A (CA) being the major flavonoid. Shibi tea is extensively used in food and medicine and has been found to provide a variety of health advantages. The benefits of Shibi tea and CA in preventing liver injury have not yet been investigated. The aim of this study was to investigate the hepatoprotective effects of extract of Shibi tea (EST) and CA in mice with carbon tetrachloride (CCl<sub>4</sub>)-induced acute liver injury. Two different concentrations of EST and CA were given to model mice by gavage for 3 days. Treatment with two concentrations of EST and CA reduced the CCl<sub>4</sub>-induced elevation of the liver index, liver histopathological injury score, alanine aminotransferase (ALT), and aspartate aminotransferase (AST). Western blotting and immunohistochemical analysis demonstrated that EST and CA regulated the oxidative stress signaling pathway protein levels of nuclear factor E2-related factor 2 (Nrf2)/heme-oxygenase-1 (HO-1), the expression of inflammatory cytokines, the phosphorylated nuclear factor-kappaB p65 (p-NF-κB)/nuclear factor-kappaB p65 (NF-κB) ratio, the phospho-p44/42 mitogen-activated protein kinase (p-MAPK), and the apoptosis-related protein levels of BCL2-associated X (Bax)/B cell leukemia/lymphoma 2 (Bcl2) in the liver. Taken together, EST and CA can protect against CCl<sub>4</sub>-induced liver injury by exerting antioxidative stress, anti-inflammation, and anti-apoptosis. |
| first_indexed | 2024-03-09T10:06:51Z |
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| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T10:06:51Z |
| publishDate | 2022-07-01 |
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| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-cf6c8ebc70284960927f9cd6593976c02023-12-01T23:05:03ZengMDPI AGNutrients2072-66432022-07-011415303710.3390/nu14153037Shibi Tea (<i>Adinandra nitida</i>) and Camellianin A Alleviate CCl<sub>4</sub>-Induced Liver Injury in C57BL-6J Mice by Attenuation of Oxidative Stress, Inflammation, and ApoptosisRuohong Chen0Yingyi Lian1Shuai Wen2Qiuhua Li3Lingli Sun4Xingfei Lai5Zhenbiao Zhang6Junquan Zhu7Linsong Tang8Ji Xuan9Erdong Yuan10Shili Sun11Tea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Resources Innovation & Utilization, Guangzhou 510640, ChinaSchool of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Plant Protein Deep Processing, Ministry of Education, South China University of Technology, Guangzhou 510641, ChinaTea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Resources Innovation & Utilization, Guangzhou 510640, ChinaTea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Resources Innovation & Utilization, Guangzhou 510640, ChinaTea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Resources Innovation & Utilization, Guangzhou 510640, ChinaTea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Resources Innovation & Utilization, Guangzhou 510640, ChinaTea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Resources Innovation & Utilization, Guangzhou 510640, ChinaGuangdong Society of Plant Protection, Guangzhou 510640, ChinaTaihongyuan Agriculture Co., Ltd., Xinyi, Maoming 525000, ChinaHospital of South China University of Technology, Guangzhou 510641, ChinaSchool of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Plant Protein Deep Processing, Ministry of Education, South China University of Technology, Guangzhou 510641, ChinaTea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Resources Innovation & Utilization, Guangzhou 510640, ChinaLiver injury is a significant public health issue nowadays. Shibi tea is a non-<i>Camellia</i> tea prepared from the dried leaves of <i>Adinandra nitida</i>, one of the plants with the greatest flavonoid concentration, with Camellianin A (CA) being the major flavonoid. Shibi tea is extensively used in food and medicine and has been found to provide a variety of health advantages. The benefits of Shibi tea and CA in preventing liver injury have not yet been investigated. The aim of this study was to investigate the hepatoprotective effects of extract of Shibi tea (EST) and CA in mice with carbon tetrachloride (CCl<sub>4</sub>)-induced acute liver injury. Two different concentrations of EST and CA were given to model mice by gavage for 3 days. Treatment with two concentrations of EST and CA reduced the CCl<sub>4</sub>-induced elevation of the liver index, liver histopathological injury score, alanine aminotransferase (ALT), and aspartate aminotransferase (AST). Western blotting and immunohistochemical analysis demonstrated that EST and CA regulated the oxidative stress signaling pathway protein levels of nuclear factor E2-related factor 2 (Nrf2)/heme-oxygenase-1 (HO-1), the expression of inflammatory cytokines, the phosphorylated nuclear factor-kappaB p65 (p-NF-κB)/nuclear factor-kappaB p65 (NF-κB) ratio, the phospho-p44/42 mitogen-activated protein kinase (p-MAPK), and the apoptosis-related protein levels of BCL2-associated X (Bax)/B cell leukemia/lymphoma 2 (Bcl2) in the liver. Taken together, EST and CA can protect against CCl<sub>4</sub>-induced liver injury by exerting antioxidative stress, anti-inflammation, and anti-apoptosis.https://www.mdpi.com/2072-6643/14/15/3037<i>Adinandra nitida</i> (Theaceae)Camellianin Aliver injuryoxidative stressinflammationanti-apoptosis |
| spellingShingle | Ruohong Chen Yingyi Lian Shuai Wen Qiuhua Li Lingli Sun Xingfei Lai Zhenbiao Zhang Junquan Zhu Linsong Tang Ji Xuan Erdong Yuan Shili Sun Shibi Tea (<i>Adinandra nitida</i>) and Camellianin A Alleviate CCl<sub>4</sub>-Induced Liver Injury in C57BL-6J Mice by Attenuation of Oxidative Stress, Inflammation, and Apoptosis Nutrients <i>Adinandra nitida</i> (Theaceae) Camellianin A liver injury oxidative stress inflammation anti-apoptosis |
| title | Shibi Tea (<i>Adinandra nitida</i>) and Camellianin A Alleviate CCl<sub>4</sub>-Induced Liver Injury in C57BL-6J Mice by Attenuation of Oxidative Stress, Inflammation, and Apoptosis |
| title_full | Shibi Tea (<i>Adinandra nitida</i>) and Camellianin A Alleviate CCl<sub>4</sub>-Induced Liver Injury in C57BL-6J Mice by Attenuation of Oxidative Stress, Inflammation, and Apoptosis |
| title_fullStr | Shibi Tea (<i>Adinandra nitida</i>) and Camellianin A Alleviate CCl<sub>4</sub>-Induced Liver Injury in C57BL-6J Mice by Attenuation of Oxidative Stress, Inflammation, and Apoptosis |
| title_full_unstemmed | Shibi Tea (<i>Adinandra nitida</i>) and Camellianin A Alleviate CCl<sub>4</sub>-Induced Liver Injury in C57BL-6J Mice by Attenuation of Oxidative Stress, Inflammation, and Apoptosis |
| title_short | Shibi Tea (<i>Adinandra nitida</i>) and Camellianin A Alleviate CCl<sub>4</sub>-Induced Liver Injury in C57BL-6J Mice by Attenuation of Oxidative Stress, Inflammation, and Apoptosis |
| title_sort | shibi tea i adinandra nitida i and camellianin a alleviate ccl sub 4 sub induced liver injury in c57bl 6j mice by attenuation of oxidative stress inflammation and apoptosis |
| topic | <i>Adinandra nitida</i> (Theaceae) Camellianin A liver injury oxidative stress inflammation anti-apoptosis |
| url | https://www.mdpi.com/2072-6643/14/15/3037 |
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