Circulating Protein Carbonyls, Antioxidant Enzymes and Related Trace Minerals among Preterms with Respiratory Distress Syndrome
Introduction: Information about oxidative stress in preterms with Respiratory Distress Syndrome (RDS) is defective, so various researches in this area are required, which may open new roads in understanding the pathogenesis of the disease, hence provide additional helpful therapeutic approaches....
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2017-07-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/10310/29085_CE(RA1)_F(T)PF1_(NE_VT_PY)_PFA(NE_SS).pdf |
| Summary: | Introduction: Information about oxidative stress in preterms
with Respiratory Distress Syndrome (RDS) is defective, so
various researches in this area are required, which may open
new roads in understanding the pathogenesis of the disease,
hence provide additional helpful therapeutic approaches.
Aim: To assess and compare the plasma level of protein carbonyls
as a marker for oxidant status and the antioxidant enzymes;
Superoxide Dismutase (SOD) and Glutathione Peroxidase (GPx)
and the related trace minerals in the form of Copper (Cu), Zinc
(Zn) and Selenium (Se) as markers for antioxidant status, in
preterms with and without RDS.
Materials and Methods: A hospital-based case-control study
was conducted on fifty-seven preterm neonates (37 preterms
with RDS and 20 preterms without RDS) admitted to neonatal
intensive care unit of Qena University Hospitals after approval
of the University Hospital Ethical Committee. Plasma protein
carbonyls assay was done using commercially available ELISA
assay kit. Plasma Cu, Zn, Se, erythrocyte SOD and GPx activities
assays were done using commercially available colorimetric
assay kits.
Results: Significant higher plasma levels of protein carbonyls
and oxidant/antioxidants ratio (protein carbonyls/{SOD+GPx})
with significant lower plasma levels of Zn, Cu, Se, erythrocyte
SOD and GPx activities were found in the preterms with RDS
when compared with the preterms without RDS (p<0.001 for all
measured markers for both groups). In terms of birth weights
and gestational ages, they were negatively correlated with
both plasma protein carbonyls and oxidant/antioxidants ratio
and positively correlated with plasma copper, zinc, selenium,
erythrocyte SOD and GPx activities in a statistically significant
manner. Non-significant correlations were found between the
measured oxidative stress markers and the severity of RDS.
Conclusion: Oxidative stress may have a contributory role in
the development of RDS among preterms. Lower birth weight
and prematurity may increase the susceptibity to oxidative
stress among such patients. |
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| ISSN: | 2249-782X 0973-709X |