Effect of Pulsatile Electric Field on Cultured Muscle Cells in Vitro
An effect of an electric field on proliferation and on differentiation of cultured muscle cells has been studied in vitro. C2C12 (the mouse myoblast cell line originated with the cross-striated muscle of C3H mouse) was exposed to electric stimuli. In the first experiment, the adhered cells were expo...
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| Format: | Article |
| Language: | English |
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International Institute of Informatics and Cybernetics
2012-02-01
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| Series: | Journal of Systemics, Cybernetics and Informatics |
| Subjects: | |
| Online Access: | http://www.iiisci.org/Journal/CV$/sci/pdfs/HBA426OR.pdf
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| _version_ | 1811306894432665600 |
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| author | Shigehiro Hashimoto Fumihiko Sato Ryuuhei Uemura Aki Nakajima |
| author_facet | Shigehiro Hashimoto Fumihiko Sato Ryuuhei Uemura Aki Nakajima |
| author_sort | Shigehiro Hashimoto |
| collection | DOAJ |
| description | An effect of an electric field on proliferation and on differentiation of cultured muscle cells has been studied in vitro. C2C12 (the mouse myoblast cell line originated with the cross-striated muscle of C3H mouse) was exposed to electric stimuli. In the first experiment, the adhered cells were exposed to the electric field between two electrodes made of platinum wire of 0.2 mm diameter dipped in the medium at 37 degrees Celsius for 72 hours. The electric pulses at a period of one second with a pulse width of 0.1 second were generated with a function generator. Variation was made on the pulse amplitude < 12 V. The number of adhered cells was counted after exposure to electric stimulation. In the second experiment, the cells were cultivated for 96 hours without electric stimulation in an incubator, after electric stimulation of 0.1 V for 72 hours. After incubation, the movement of myotubes was observed with electric stimulation at a period of one second with a pulse width of one millisecond of 30 V. The experimental results show that cells adhere and proliferate under electric pulses lower than 8 V, and that differentiation accelerates with the electric pulses of 0.1 V. |
| first_indexed | 2024-04-13T08:53:40Z |
| format | Article |
| id | doaj.art-cf70074140454ff4801382180464b9f2 |
| institution | Directory Open Access Journal |
| issn | 1690-4524 |
| language | English |
| last_indexed | 2024-04-13T08:53:40Z |
| publishDate | 2012-02-01 |
| publisher | International Institute of Informatics and Cybernetics |
| record_format | Article |
| series | Journal of Systemics, Cybernetics and Informatics |
| spelling | doaj.art-cf70074140454ff4801382180464b9f22022-12-22T02:53:24ZengInternational Institute of Informatics and CyberneticsJournal of Systemics, Cybernetics and Informatics1690-45242012-02-0110116Effect of Pulsatile Electric Field on Cultured Muscle Cells in VitroShigehiro Hashimoto0Fumihiko Sato1Ryuuhei Uemura2Aki Nakajima3 Kogakuin University Kogakuin University Osaka Institute of Technology Osaka Institute of Technology An effect of an electric field on proliferation and on differentiation of cultured muscle cells has been studied in vitro. C2C12 (the mouse myoblast cell line originated with the cross-striated muscle of C3H mouse) was exposed to electric stimuli. In the first experiment, the adhered cells were exposed to the electric field between two electrodes made of platinum wire of 0.2 mm diameter dipped in the medium at 37 degrees Celsius for 72 hours. The electric pulses at a period of one second with a pulse width of 0.1 second were generated with a function generator. Variation was made on the pulse amplitude < 12 V. The number of adhered cells was counted after exposure to electric stimulation. In the second experiment, the cells were cultivated for 96 hours without electric stimulation in an incubator, after electric stimulation of 0.1 V for 72 hours. After incubation, the movement of myotubes was observed with electric stimulation at a period of one second with a pulse width of one millisecond of 30 V. The experimental results show that cells adhere and proliferate under electric pulses lower than 8 V, and that differentiation accelerates with the electric pulses of 0.1 V.http://www.iiisci.org/Journal/CV$/sci/pdfs/HBA426OR.pdf Cell CultureBiomedical EngineeringElectric FieldDifferentiation And ProliferationMuscle Cells |
| spellingShingle | Shigehiro Hashimoto Fumihiko Sato Ryuuhei Uemura Aki Nakajima Effect of Pulsatile Electric Field on Cultured Muscle Cells in Vitro Journal of Systemics, Cybernetics and Informatics Cell Culture Biomedical Engineering Electric Field Differentiation And Proliferation Muscle Cells |
| title | Effect of Pulsatile Electric Field on Cultured Muscle Cells in Vitro |
| title_full | Effect of Pulsatile Electric Field on Cultured Muscle Cells in Vitro |
| title_fullStr | Effect of Pulsatile Electric Field on Cultured Muscle Cells in Vitro |
| title_full_unstemmed | Effect of Pulsatile Electric Field on Cultured Muscle Cells in Vitro |
| title_short | Effect of Pulsatile Electric Field on Cultured Muscle Cells in Vitro |
| title_sort | effect of pulsatile electric field on cultured muscle cells in vitro |
| topic | Cell Culture Biomedical Engineering Electric Field Differentiation And Proliferation Muscle Cells |
| url | http://www.iiisci.org/Journal/CV$/sci/pdfs/HBA426OR.pdf
|
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