Comparison of in vitro properties of periodontal ligament stem cells derived from permanent and deciduous teeth
Background. Stem cells have contributed to the development of tissue-engineered-based regenerative periodontal therapies. In order to find the best stem cell sources for such therapies, the biologic properties of stem cells isolated from periodontal ligaments (PDL) of deciduous (De...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Tabriz University of Medical Sciences
2017-09-01
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| Series: | Journal of Dental Research, Dental Clinics, Dental Prospects |
| Subjects: | |
| Online Access: | http://joddd.tbzmed.ac.ir/PDF/joddd-11-140.pdf |
| Summary: | Background. Stem cells have contributed to the development of
tissue-engineered-based regenerative periodontal therapies. In order to find the best stem
cell sources for such therapies, the biologic properties of stem cells isolated from
periodontal ligaments (PDL) of deciduous (DePDLSC) and permanent (PePDLSC) teeth were
comparatively evaluated. Methods. PDL stem cells were isolated from six sound fully erupted
premolars and six deciduous canines of healthy subjects. In vitro biologic characteristics
such as colony formation, viability, stem cell marker identification and osteogenic
differentiation (using alkaline phosphatase analysis and Alizarin red staining) were
comparatively assessed using one-way ANOVA and post hoc Tukey tests using SPSS 13.0. Results.
Stem cell populations isolated from both groups were CD105+ and CD90+ and CD45‒. No
statistically significant differences were found in stem cell markers, colony formation and
viability. Both groups were capable of osteogenic differentiation. However, alkaline
phosphatase activity test showed a statistically significant difference, with PePDLSC
exhibiting higher alkaline phosphatase activity (P=0.000). No statistically significant
difference was seen in quantitative alizarine red staining (P=0.559). Conclusion. Mesenchymal
stem cells of PDL could successfully be isolated from permanent and deciduous teeth. A minor
difference was observed in the osteogenic properties of the two cell types, which might affect
their future clinical applications. |
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| ISSN: | 2008-210X 2008-2118 |