Potential Antimicrobe Producer of Endophytic Bacteria from Yellow Root Plant (Arcangelisia flava (L.)) Originated from Enggano Island
Exploration studies of endophytic bacteria from Arcangelisia flava (L.) and their potential have not much been conducted. This research aims to explore and characterize the antimicrobial activity of endophytic bacteria in A. flava against pathogenic bacteria. This research consists of several steps...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2022-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2022/6435202 |
| _version_ | 1797803085650722816 |
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| author | null Sipriyadi null Masrukhin Risky Hadi Wibowo Welly Darwis Salprima Yudha Ismu Purnaningsih Resli Siboro |
| author_facet | null Sipriyadi null Masrukhin Risky Hadi Wibowo Welly Darwis Salprima Yudha Ismu Purnaningsih Resli Siboro |
| author_sort | null Sipriyadi |
| collection | DOAJ |
| description | Exploration studies of endophytic bacteria from Arcangelisia flava (L.) and their potential have not much been conducted. This research aims to explore and characterize the antimicrobial activity of endophytic bacteria in A. flava against pathogenic bacteria. This research consists of several steps including the isolation of bacteria, screening of the antimicrobial activity assay using the dual cross streak method, molecular identification through 16s rDNA analysis, and characterization of bioactive compound production through PKS-NRPS gene detection and GC-MS analysis. There are 29 endophytic bacteria that were successfully isolated from A. flava. The antimicrobial activity showed that there are four potential isolates AKEBG21, AKEBG23, AKEBG25, and AKEBG28 that can inhibit the growth of pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The 16S rDNA sequence analysis showed that these isolates are identified as Bacillus cereus. These four isolates are identified as able to produce the bioactive compounds through the detection of polyketide synthase (PKS) and nonribosomal peptide synthase (NRPS)-encoding genes. B. cereus AKEBG23 has the highest inhibition against pathogenic bacteria, and according to the GC-MS analysis, five major compounds are allegedly involved in its antimicrobial activity such as butylated hydroxytoluene (BHT), diisooctyl phthalate, E-15-heptadecenal, 1-heneicosanol, and E-14-hexadecenal. This result suggested that B. cereus AKEBG23 as the endophytic bacterium from A. flava has a beneficial role as well as the plant itself. The bacterium produces several bioactive compounds that are allegedly involved in its antimicrobial activity against pathogenic bacteria. |
| first_indexed | 2024-03-13T05:16:34Z |
| format | Article |
| id | doaj.art-cf715696e5e848dfb67be173e02e18b1 |
| institution | Directory Open Access Journal |
| issn | 1687-9198 |
| language | English |
| last_indexed | 2024-03-13T05:16:34Z |
| publishDate | 2022-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj.art-cf715696e5e848dfb67be173e02e18b12023-06-16T00:00:01ZengHindawi LimitedInternational Journal of Microbiology1687-91982022-01-01202210.1155/2022/6435202Potential Antimicrobe Producer of Endophytic Bacteria from Yellow Root Plant (Arcangelisia flava (L.)) Originated from Enggano Islandnull Sipriyadi0null Masrukhin1Risky Hadi Wibowo2Welly Darwis3Salprima Yudha4Ismu Purnaningsih5Resli Siboro6Faculty of Mathematics and Natural ScienceResearch Center for Biosystematics and EvolutionFaculty of Mathematics and Natural ScienceMaster Program of BiologyChemistry DepartmentDirectorate for Scientific CollectionMaster Program of BiologyExploration studies of endophytic bacteria from Arcangelisia flava (L.) and their potential have not much been conducted. This research aims to explore and characterize the antimicrobial activity of endophytic bacteria in A. flava against pathogenic bacteria. This research consists of several steps including the isolation of bacteria, screening of the antimicrobial activity assay using the dual cross streak method, molecular identification through 16s rDNA analysis, and characterization of bioactive compound production through PKS-NRPS gene detection and GC-MS analysis. There are 29 endophytic bacteria that were successfully isolated from A. flava. The antimicrobial activity showed that there are four potential isolates AKEBG21, AKEBG23, AKEBG25, and AKEBG28 that can inhibit the growth of pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The 16S rDNA sequence analysis showed that these isolates are identified as Bacillus cereus. These four isolates are identified as able to produce the bioactive compounds through the detection of polyketide synthase (PKS) and nonribosomal peptide synthase (NRPS)-encoding genes. B. cereus AKEBG23 has the highest inhibition against pathogenic bacteria, and according to the GC-MS analysis, five major compounds are allegedly involved in its antimicrobial activity such as butylated hydroxytoluene (BHT), diisooctyl phthalate, E-15-heptadecenal, 1-heneicosanol, and E-14-hexadecenal. This result suggested that B. cereus AKEBG23 as the endophytic bacterium from A. flava has a beneficial role as well as the plant itself. The bacterium produces several bioactive compounds that are allegedly involved in its antimicrobial activity against pathogenic bacteria.http://dx.doi.org/10.1155/2022/6435202 |
| spellingShingle | null Sipriyadi null Masrukhin Risky Hadi Wibowo Welly Darwis Salprima Yudha Ismu Purnaningsih Resli Siboro Potential Antimicrobe Producer of Endophytic Bacteria from Yellow Root Plant (Arcangelisia flava (L.)) Originated from Enggano Island International Journal of Microbiology |
| title | Potential Antimicrobe Producer of Endophytic Bacteria from Yellow Root Plant (Arcangelisia flava (L.)) Originated from Enggano Island |
| title_full | Potential Antimicrobe Producer of Endophytic Bacteria from Yellow Root Plant (Arcangelisia flava (L.)) Originated from Enggano Island |
| title_fullStr | Potential Antimicrobe Producer of Endophytic Bacteria from Yellow Root Plant (Arcangelisia flava (L.)) Originated from Enggano Island |
| title_full_unstemmed | Potential Antimicrobe Producer of Endophytic Bacteria from Yellow Root Plant (Arcangelisia flava (L.)) Originated from Enggano Island |
| title_short | Potential Antimicrobe Producer of Endophytic Bacteria from Yellow Root Plant (Arcangelisia flava (L.)) Originated from Enggano Island |
| title_sort | potential antimicrobe producer of endophytic bacteria from yellow root plant arcangelisia flava l originated from enggano island |
| url | http://dx.doi.org/10.1155/2022/6435202 |
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