Characterization of the Protective Efficacy Against QX Strain of a Recombinant Infectious Bronchitis Virus With H120 Backbone and QX Spike Gene
Infectious bronchitis virus (IBV) has been prevalent in chicken farms for many years, and its control relies on extensive vaccine administration. The continuous emergence of new variants and the low cross-protection efficiency prompt the development of new vaccines. In this study, we develop a rever...
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| Format: | Article |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.883642/full |
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| author | Wenlian Weng Qingyan Liu Qingyan Liu Wenxiang Xue Huan Wang Shouguo Fang Yingjie Sun Lei Tan Cuiping Song Xusheng Qiu Weiwei Liu Chan Ding Chan Ding Ying Liao |
| author_facet | Wenlian Weng Qingyan Liu Qingyan Liu Wenxiang Xue Huan Wang Shouguo Fang Yingjie Sun Lei Tan Cuiping Song Xusheng Qiu Weiwei Liu Chan Ding Chan Ding Ying Liao |
| author_sort | Wenlian Weng |
| collection | DOAJ |
| description | Infectious bronchitis virus (IBV) has been prevalent in chicken farms for many years, and its control relies on extensive vaccine administration. The continuous emergence of new variants and the low cross-protection efficiency prompt the development of new vaccines. In this study, we develop a reverse genetics technique based on the classical vaccine strain H120 genome, via in vitro ligation method. Using the H120 genome as the backbone, we constructed the recombinant virus rH120-QX(S) by replacing the H120 S gene with the QX S gene, a prevalent strain in China. Biological characteristics of the rH120-QX(S) virus, such as 50% egg lethal dose (ELD50), 50% egg infectious dose (EID50), dwarf embryo, growth curve, and genetic stability, are measured, which are comparable to the parental virus H120. There are no clinical symptoms and tissue lesions in the trachea and kidney in the rH120-QX(S)-infected specific-pathogen-free (SPF) chickens, demonstrating that this recombinant virus does not confer pathogenicity. Furthermore, protection studies show that there is 100% homologous protection of rH120-QX(S) to the virulent QX strain, as shown by the absence of clinical signs and no lethality. Taken together, our results demonstrate that swapping the S gene onto the H120 genetic backbone is a precise and effective way to produce genetically defined IBV vaccine candidates. |
| first_indexed | 2024-12-12T16:24:34Z |
| format | Article |
| id | doaj.art-cf71cac54b604ce985b52ddb49e71cad |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T16:24:34Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-cf71cac54b604ce985b52ddb49e71cad2022-12-22T00:18:55ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-06-011310.3389/fmicb.2022.883642883642Characterization of the Protective Efficacy Against QX Strain of a Recombinant Infectious Bronchitis Virus With H120 Backbone and QX Spike GeneWenlian Weng0Qingyan Liu1Qingyan Liu2Wenxiang Xue3Huan Wang4Shouguo Fang5Yingjie Sun6Lei Tan7Cuiping Song8Xusheng Qiu9Weiwei Liu10Chan Ding11Chan Ding12Ying Liao13Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, ChinaDepartment of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, ChinaCollege of Agriculture, College of Animal Sciences, Yangtze University, Jingzhou, ChinaDepartment of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, ChinaDepartment of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, ChinaCollege of Agriculture, College of Animal Sciences, Yangtze University, Jingzhou, ChinaDepartment of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, ChinaDepartment of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, ChinaDepartment of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, ChinaDepartment of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, ChinaDepartment of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, ChinaDepartment of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaDepartment of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, ChinaInfectious bronchitis virus (IBV) has been prevalent in chicken farms for many years, and its control relies on extensive vaccine administration. The continuous emergence of new variants and the low cross-protection efficiency prompt the development of new vaccines. In this study, we develop a reverse genetics technique based on the classical vaccine strain H120 genome, via in vitro ligation method. Using the H120 genome as the backbone, we constructed the recombinant virus rH120-QX(S) by replacing the H120 S gene with the QX S gene, a prevalent strain in China. Biological characteristics of the rH120-QX(S) virus, such as 50% egg lethal dose (ELD50), 50% egg infectious dose (EID50), dwarf embryo, growth curve, and genetic stability, are measured, which are comparable to the parental virus H120. There are no clinical symptoms and tissue lesions in the trachea and kidney in the rH120-QX(S)-infected specific-pathogen-free (SPF) chickens, demonstrating that this recombinant virus does not confer pathogenicity. Furthermore, protection studies show that there is 100% homologous protection of rH120-QX(S) to the virulent QX strain, as shown by the absence of clinical signs and no lethality. Taken together, our results demonstrate that swapping the S gene onto the H120 genetic backbone is a precise and effective way to produce genetically defined IBV vaccine candidates.https://www.frontiersin.org/articles/10.3389/fmicb.2022.883642/fullinfectious bronchitis virusvaccine H120QXS genereverse geneticsrH120-QX(S) |
| spellingShingle | Wenlian Weng Qingyan Liu Qingyan Liu Wenxiang Xue Huan Wang Shouguo Fang Yingjie Sun Lei Tan Cuiping Song Xusheng Qiu Weiwei Liu Chan Ding Chan Ding Ying Liao Characterization of the Protective Efficacy Against QX Strain of a Recombinant Infectious Bronchitis Virus With H120 Backbone and QX Spike Gene Frontiers in Microbiology infectious bronchitis virus vaccine H120 QX S gene reverse genetics rH120-QX(S) |
| title | Characterization of the Protective Efficacy Against QX Strain of a Recombinant Infectious Bronchitis Virus With H120 Backbone and QX Spike Gene |
| title_full | Characterization of the Protective Efficacy Against QX Strain of a Recombinant Infectious Bronchitis Virus With H120 Backbone and QX Spike Gene |
| title_fullStr | Characterization of the Protective Efficacy Against QX Strain of a Recombinant Infectious Bronchitis Virus With H120 Backbone and QX Spike Gene |
| title_full_unstemmed | Characterization of the Protective Efficacy Against QX Strain of a Recombinant Infectious Bronchitis Virus With H120 Backbone and QX Spike Gene |
| title_short | Characterization of the Protective Efficacy Against QX Strain of a Recombinant Infectious Bronchitis Virus With H120 Backbone and QX Spike Gene |
| title_sort | characterization of the protective efficacy against qx strain of a recombinant infectious bronchitis virus with h120 backbone and qx spike gene |
| topic | infectious bronchitis virus vaccine H120 QX S gene reverse genetics rH120-QX(S) |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.883642/full |
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