Fluorescent microangiography is a novel and widely applicable technique for delineating the renal microvasculature.
Rarefaction of the renal microvasculature correlates with declining kidney function. However, current technologies commonly used for its evaluation are limited by their reliance on endothelial cell antigen expression and assessment in two dimensions. We set out to establish a widely applicable and u...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3184963?pdf=render |
| _version_ | 1819168235571904512 |
|---|---|
| author | Andrew Advani Kim A Connelly Darren A Yuen Yanling Zhang Suzanne L Advani Judy Trogadis M Golam Kabir Etai Shachar Michael A Kuliszewski Howard Leong-Poi Duncan J Stewart Richard E Gilbert |
| author_facet | Andrew Advani Kim A Connelly Darren A Yuen Yanling Zhang Suzanne L Advani Judy Trogadis M Golam Kabir Etai Shachar Michael A Kuliszewski Howard Leong-Poi Duncan J Stewart Richard E Gilbert |
| author_sort | Andrew Advani |
| collection | DOAJ |
| description | Rarefaction of the renal microvasculature correlates with declining kidney function. However, current technologies commonly used for its evaluation are limited by their reliance on endothelial cell antigen expression and assessment in two dimensions. We set out to establish a widely applicable and unbiased optical sectioning method to enable three dimensional imaging and reconstruction of the renal microvessels based on their luminal filling. The kidneys of subtotally nephrectomized (SNx) rats and their sham-operated counterparts were subjected to either routine two-dimensional immunohistochemistry or the novel technique of fluorescent microangiography (FMA). The latter was achieved by perfusion of the kidney with an agarose suspension of fluorescent polystyrene microspheres followed by optical sectioning of 200 µm thick cross-sections using a confocal microscope. The fluorescent microangiography method enabled the three-dimensional reconstruction of virtual microvascular casts and confirmed a reduction in both glomerular and peritubular capillary density in the kidneys of SNx rats, despite an overall increase in glomerular volume. FMA is an uncomplicated technique for evaluating the renal microvasculature that circumvents many of the limitations imposed by conventional analysis of two-dimensional tissue sections. |
| first_indexed | 2024-12-22T19:00:23Z |
| format | Article |
| id | doaj.art-cf75e6c10b094397a793ced8b01c090b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T19:00:23Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-cf75e6c10b094397a793ced8b01c090b2022-12-21T18:15:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01610e2469510.1371/journal.pone.0024695Fluorescent microangiography is a novel and widely applicable technique for delineating the renal microvasculature.Andrew AdvaniKim A ConnellyDarren A YuenYanling ZhangSuzanne L AdvaniJudy TrogadisM Golam KabirEtai ShacharMichael A KuliszewskiHoward Leong-PoiDuncan J StewartRichard E GilbertRarefaction of the renal microvasculature correlates with declining kidney function. However, current technologies commonly used for its evaluation are limited by their reliance on endothelial cell antigen expression and assessment in two dimensions. We set out to establish a widely applicable and unbiased optical sectioning method to enable three dimensional imaging and reconstruction of the renal microvessels based on their luminal filling. The kidneys of subtotally nephrectomized (SNx) rats and their sham-operated counterparts were subjected to either routine two-dimensional immunohistochemistry or the novel technique of fluorescent microangiography (FMA). The latter was achieved by perfusion of the kidney with an agarose suspension of fluorescent polystyrene microspheres followed by optical sectioning of 200 µm thick cross-sections using a confocal microscope. The fluorescent microangiography method enabled the three-dimensional reconstruction of virtual microvascular casts and confirmed a reduction in both glomerular and peritubular capillary density in the kidneys of SNx rats, despite an overall increase in glomerular volume. FMA is an uncomplicated technique for evaluating the renal microvasculature that circumvents many of the limitations imposed by conventional analysis of two-dimensional tissue sections.http://europepmc.org/articles/PMC3184963?pdf=render |
| spellingShingle | Andrew Advani Kim A Connelly Darren A Yuen Yanling Zhang Suzanne L Advani Judy Trogadis M Golam Kabir Etai Shachar Michael A Kuliszewski Howard Leong-Poi Duncan J Stewart Richard E Gilbert Fluorescent microangiography is a novel and widely applicable technique for delineating the renal microvasculature. PLoS ONE |
| title | Fluorescent microangiography is a novel and widely applicable technique for delineating the renal microvasculature. |
| title_full | Fluorescent microangiography is a novel and widely applicable technique for delineating the renal microvasculature. |
| title_fullStr | Fluorescent microangiography is a novel and widely applicable technique for delineating the renal microvasculature. |
| title_full_unstemmed | Fluorescent microangiography is a novel and widely applicable technique for delineating the renal microvasculature. |
| title_short | Fluorescent microangiography is a novel and widely applicable technique for delineating the renal microvasculature. |
| title_sort | fluorescent microangiography is a novel and widely applicable technique for delineating the renal microvasculature |
| url | http://europepmc.org/articles/PMC3184963?pdf=render |
| work_keys_str_mv | AT andrewadvani fluorescentmicroangiographyisanovelandwidelyapplicabletechniquefordelineatingtherenalmicrovasculature AT kimaconnelly fluorescentmicroangiographyisanovelandwidelyapplicabletechniquefordelineatingtherenalmicrovasculature AT darrenayuen fluorescentmicroangiographyisanovelandwidelyapplicabletechniquefordelineatingtherenalmicrovasculature AT yanlingzhang fluorescentmicroangiographyisanovelandwidelyapplicabletechniquefordelineatingtherenalmicrovasculature AT suzanneladvani fluorescentmicroangiographyisanovelandwidelyapplicabletechniquefordelineatingtherenalmicrovasculature AT judytrogadis fluorescentmicroangiographyisanovelandwidelyapplicabletechniquefordelineatingtherenalmicrovasculature AT mgolamkabir fluorescentmicroangiographyisanovelandwidelyapplicabletechniquefordelineatingtherenalmicrovasculature AT etaishachar fluorescentmicroangiographyisanovelandwidelyapplicabletechniquefordelineatingtherenalmicrovasculature AT michaelakuliszewski fluorescentmicroangiographyisanovelandwidelyapplicabletechniquefordelineatingtherenalmicrovasculature AT howardleongpoi fluorescentmicroangiographyisanovelandwidelyapplicabletechniquefordelineatingtherenalmicrovasculature AT duncanjstewart fluorescentmicroangiographyisanovelandwidelyapplicabletechniquefordelineatingtherenalmicrovasculature AT richardegilbert fluorescentmicroangiographyisanovelandwidelyapplicabletechniquefordelineatingtherenalmicrovasculature |