A Two-Stage Adaptive Laboratory Evolution Strategy to Enhance Docosahexaenoic Acid Synthesis in Oleaginous Thraustochytrid
Thraustochytrid is a promising algal oil resource with the potential to meet the demand for docosahexaenoic acid (DHA). However, oils with high DHA content produced by genetic modified thraustochytrids are not accepted by the food and pharmaceutical industries in many countries. Therefore, in order...
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2021.795491/full |
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| author | Sen Wang Sen Wang Sen Wang Sen Wang Sen Wang Sen Wang Weijian Wan Weijian Wan Weijian Wan Weijian Wan Zhuojun Wang Zhuojun Wang Zhuojun Wang Zhuojun Wang Zhuojun Wang Huidan Zhang Huidan Zhang Huidan Zhang Huidan Zhang Huidan Zhang Huidan Zhang Huan Liu Huan Liu Huan Liu Huan Liu Huan Liu Huan Liu K. K. I. U. Arunakumara Qiu Cui Qiu Cui Qiu Cui Qiu Cui Qiu Cui Qiu Cui Qiu Cui Xiaojin Song Xiaojin Song Xiaojin Song Xiaojin Song Xiaojin Song Xiaojin Song Xiaojin Song Xiaojin Song |
| author_facet | Sen Wang Sen Wang Sen Wang Sen Wang Sen Wang Sen Wang Weijian Wan Weijian Wan Weijian Wan Weijian Wan Zhuojun Wang Zhuojun Wang Zhuojun Wang Zhuojun Wang Zhuojun Wang Huidan Zhang Huidan Zhang Huidan Zhang Huidan Zhang Huidan Zhang Huidan Zhang Huan Liu Huan Liu Huan Liu Huan Liu Huan Liu Huan Liu K. K. I. U. Arunakumara Qiu Cui Qiu Cui Qiu Cui Qiu Cui Qiu Cui Qiu Cui Qiu Cui Xiaojin Song Xiaojin Song Xiaojin Song Xiaojin Song Xiaojin Song Xiaojin Song Xiaojin Song Xiaojin Song |
| author_sort | Sen Wang |
| collection | DOAJ |
| description | Thraustochytrid is a promising algal oil resource with the potential to meet the demand for docosahexaenoic acid (DHA). However, oils with high DHA content produced by genetic modified thraustochytrids are not accepted by the food and pharmaceutical industries in many countries. Therefore, in order to obtain non-transgenic strains with high DHA content, a two-stage adaptive laboratory evolution (ALE) strategy was applied to the thraustochytrid Aurantiochytrium sp. Heavy-ion irradiation technique was first used before the ALE to increase the genetic diversity of strains, and then two-step ALE: low temperature based ALE and ACCase inhibitor quizalofop-p-ethyl based ALE were employed in enhancing the DHA production. Using this strategy, the end-point strain E-81 with a DHA content 51% higher than that of the parental strain was obtained. The performance of E-81 strain was further analyzed by component analysis and quantitative real-time PCR. The results showed that the enhanced in lipid content was due to the up-regulated expression of key enzymes in lipid accumulation, while the increase in DHA content was due to the increased transcriptional levels of polyunsaturated fatty acid synthase. This study demonstrated a non-genetic approach to enhance lipid and DHA content in non-model industrial oleaginous strains. |
| first_indexed | 2024-12-13T23:27:22Z |
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| last_indexed | 2024-12-13T23:27:22Z |
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| spelling | doaj.art-cf79cd7e863547bb8f7b119256004e142022-12-21T23:27:31ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2021-12-01810.3389/fnut.2021.795491795491A Two-Stage Adaptive Laboratory Evolution Strategy to Enhance Docosahexaenoic Acid Synthesis in Oleaginous ThraustochytridSen Wang0Sen Wang1Sen Wang2Sen Wang3Sen Wang4Sen Wang5Weijian Wan6Weijian Wan7Weijian Wan8Weijian Wan9Zhuojun Wang10Zhuojun Wang11Zhuojun Wang12Zhuojun Wang13Zhuojun Wang14Huidan Zhang15Huidan Zhang16Huidan Zhang17Huidan Zhang18Huidan Zhang19Huidan Zhang20Huan Liu21Huan Liu22Huan Liu23Huan Liu24Huan Liu25Huan Liu26K. K. I. U. Arunakumara27Qiu Cui28Qiu Cui29Qiu Cui30Qiu Cui31Qiu Cui32Qiu Cui33Qiu Cui34Xiaojin Song35Xiaojin Song36Xiaojin Song37Xiaojin Song38Xiaojin Song39Xiaojin Song40Xiaojin Song41Xiaojin Song42Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaShandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaShandong Energy Institute, Qingdao, ChinaQingdao New Energy Shandong Laboratory, Qingdao, ChinaShandong Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaQingdao Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaKey Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaShandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaShandong Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaQingdao Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaKey Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaShandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaShandong Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaQingdao Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaKey Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaShandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaShandong Energy Institute, Qingdao, ChinaQingdao New Energy Shandong Laboratory, Qingdao, ChinaShandong Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaQingdao Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaKey Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaShandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaShandong Energy Institute, Qingdao, ChinaQingdao New Energy Shandong Laboratory, Qingdao, ChinaShandong Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaQingdao Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaDepartment of Crop Science, Faculty of Agriculture, University of Ruhuna, Kamburupitiya, Sri LankaKey Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaShandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaShandong Energy Institute, Qingdao, ChinaQingdao New Energy Shandong Laboratory, Qingdao, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaShandong Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaQingdao Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaKey Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaShandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaShandong Energy Institute, Qingdao, ChinaQingdao New Energy Shandong Laboratory, Qingdao, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaShandong Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaQingdao Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaThraustochytrid is a promising algal oil resource with the potential to meet the demand for docosahexaenoic acid (DHA). However, oils with high DHA content produced by genetic modified thraustochytrids are not accepted by the food and pharmaceutical industries in many countries. Therefore, in order to obtain non-transgenic strains with high DHA content, a two-stage adaptive laboratory evolution (ALE) strategy was applied to the thraustochytrid Aurantiochytrium sp. Heavy-ion irradiation technique was first used before the ALE to increase the genetic diversity of strains, and then two-step ALE: low temperature based ALE and ACCase inhibitor quizalofop-p-ethyl based ALE were employed in enhancing the DHA production. Using this strategy, the end-point strain E-81 with a DHA content 51% higher than that of the parental strain was obtained. The performance of E-81 strain was further analyzed by component analysis and quantitative real-time PCR. The results showed that the enhanced in lipid content was due to the up-regulated expression of key enzymes in lipid accumulation, while the increase in DHA content was due to the increased transcriptional levels of polyunsaturated fatty acid synthase. This study demonstrated a non-genetic approach to enhance lipid and DHA content in non-model industrial oleaginous strains.https://www.frontiersin.org/articles/10.3389/fnut.2021.795491/fulladaptive laboratory evolutionthraustochytrid Aurantiochytriumdocosahexaenoic acidheavy-ion irradiationlipid accumulation |
| spellingShingle | Sen Wang Sen Wang Sen Wang Sen Wang Sen Wang Sen Wang Weijian Wan Weijian Wan Weijian Wan Weijian Wan Zhuojun Wang Zhuojun Wang Zhuojun Wang Zhuojun Wang Zhuojun Wang Huidan Zhang Huidan Zhang Huidan Zhang Huidan Zhang Huidan Zhang Huidan Zhang Huan Liu Huan Liu Huan Liu Huan Liu Huan Liu Huan Liu K. K. I. U. Arunakumara Qiu Cui Qiu Cui Qiu Cui Qiu Cui Qiu Cui Qiu Cui Qiu Cui Xiaojin Song Xiaojin Song Xiaojin Song Xiaojin Song Xiaojin Song Xiaojin Song Xiaojin Song Xiaojin Song A Two-Stage Adaptive Laboratory Evolution Strategy to Enhance Docosahexaenoic Acid Synthesis in Oleaginous Thraustochytrid Frontiers in Nutrition adaptive laboratory evolution thraustochytrid Aurantiochytrium docosahexaenoic acid heavy-ion irradiation lipid accumulation |
| title | A Two-Stage Adaptive Laboratory Evolution Strategy to Enhance Docosahexaenoic Acid Synthesis in Oleaginous Thraustochytrid |
| title_full | A Two-Stage Adaptive Laboratory Evolution Strategy to Enhance Docosahexaenoic Acid Synthesis in Oleaginous Thraustochytrid |
| title_fullStr | A Two-Stage Adaptive Laboratory Evolution Strategy to Enhance Docosahexaenoic Acid Synthesis in Oleaginous Thraustochytrid |
| title_full_unstemmed | A Two-Stage Adaptive Laboratory Evolution Strategy to Enhance Docosahexaenoic Acid Synthesis in Oleaginous Thraustochytrid |
| title_short | A Two-Stage Adaptive Laboratory Evolution Strategy to Enhance Docosahexaenoic Acid Synthesis in Oleaginous Thraustochytrid |
| title_sort | two stage adaptive laboratory evolution strategy to enhance docosahexaenoic acid synthesis in oleaginous thraustochytrid |
| topic | adaptive laboratory evolution thraustochytrid Aurantiochytrium docosahexaenoic acid heavy-ion irradiation lipid accumulation |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2021.795491/full |
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