The Effects of TiO<sub>2</sub> Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines
There have been many studies on improving the efficacy of cisplatin and on identifying safe compounds that can overcome multi-drug resistance (MDR) acquired by cancer cells. Our previous research showed that polyethylene glycol-modified titanium dioxide nanoparticles (TiO<sub>2</sub> PEG...
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MDPI AG
2020-01-01
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| Series: | International Journal of Molecular Sciences |
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| author | Basma Salama El-Said El-Sherbini Gehad El-Sayed Mohamed El-Adl Koki Kanehira Akiyoshi Taniguchi |
| author_facet | Basma Salama El-Said El-Sherbini Gehad El-Sayed Mohamed El-Adl Koki Kanehira Akiyoshi Taniguchi |
| author_sort | Basma Salama |
| collection | DOAJ |
| description | There have been many studies on improving the efficacy of cisplatin and on identifying safe compounds that can overcome multi-drug resistance (MDR) acquired by cancer cells. Our previous research showed that polyethylene glycol-modified titanium dioxide nanoparticles (TiO<sub>2</sub> PEG NPs) affect cell membrane receptors, resulting in their aggregation, altered localization and downregulation. TiO<sub>2</sub> PEG NPs may affect P-glycoprotein (P-gp), a membrane efflux channel involved in MDR. In this study, we investigated the effect of TiO<sub>2</sub> PEG NPs on cisplatin cytotoxicity. We used HepG2 cells, which highly express P-gp and A431 cells, which show low expression of P-gp. The results showed that 10 µg/mL 100 nm TiO<sub>2</sub> PEG NPs increased intracellular cisplatin levels and cytotoxicity in HepG2 cells but not in A431 cells. TiO<sub>2</sub> PEG NPs treatment decreased the expression level of P-gp in HepG2 cells. Our findings indicate that TiO<sub>2</sub> PEG NPs enhance cisplatin cytotoxicity by down regulating P-gp and that TiO<sub>2</sub> PEG NPs are promising candidates for inhibiting P-gp and reversing drug resistance acquired by cancer cells. |
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| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T09:29:26Z |
| publishDate | 2020-01-01 |
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| spelling | doaj.art-cf7f0d7df4b64f7c883c18e74bec496b2022-12-22T02:52:19ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-01-0121260510.3390/ijms21020605ijms21020605The Effects of TiO<sub>2</sub> Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell LinesBasma Salama0El-Said El-Sherbini1Gehad El-Sayed2Mohamed El-Adl3Koki Kanehira4Akiyoshi Taniguchi5Cellular Functional Nano Biomaterials Group, Research Center for Biomaterials, National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki 305-0044, JapanDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, 60 El Gomhouria St., Mansoura, Dakahlia Governorate 35516, EgyptDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, 60 El Gomhouria St., Mansoura, Dakahlia Governorate 35516, EgyptDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, 60 El Gomhouria St., Mansoura, Dakahlia Governorate 35516, EgyptCellular Functional Nano Biomaterials Group, Research Center for Biomaterials, National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki 305-0044, JapanCellular Functional Nano Biomaterials Group, Research Center for Biomaterials, National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki 305-0044, JapanThere have been many studies on improving the efficacy of cisplatin and on identifying safe compounds that can overcome multi-drug resistance (MDR) acquired by cancer cells. Our previous research showed that polyethylene glycol-modified titanium dioxide nanoparticles (TiO<sub>2</sub> PEG NPs) affect cell membrane receptors, resulting in their aggregation, altered localization and downregulation. TiO<sub>2</sub> PEG NPs may affect P-glycoprotein (P-gp), a membrane efflux channel involved in MDR. In this study, we investigated the effect of TiO<sub>2</sub> PEG NPs on cisplatin cytotoxicity. We used HepG2 cells, which highly express P-gp and A431 cells, which show low expression of P-gp. The results showed that 10 µg/mL 100 nm TiO<sub>2</sub> PEG NPs increased intracellular cisplatin levels and cytotoxicity in HepG2 cells but not in A431 cells. TiO<sub>2</sub> PEG NPs treatment decreased the expression level of P-gp in HepG2 cells. Our findings indicate that TiO<sub>2</sub> PEG NPs enhance cisplatin cytotoxicity by down regulating P-gp and that TiO<sub>2</sub> PEG NPs are promising candidates for inhibiting P-gp and reversing drug resistance acquired by cancer cells.https://www.mdpi.com/1422-0067/21/2/605titanium dioxide nanoparticlescisplatincytotoxicitydrug resistancep-glycoprotein |
| spellingShingle | Basma Salama El-Said El-Sherbini Gehad El-Sayed Mohamed El-Adl Koki Kanehira Akiyoshi Taniguchi The Effects of TiO<sub>2</sub> Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines International Journal of Molecular Sciences titanium dioxide nanoparticles cisplatin cytotoxicity drug resistance p-glycoprotein |
| title | The Effects of TiO<sub>2</sub> Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines |
| title_full | The Effects of TiO<sub>2</sub> Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines |
| title_fullStr | The Effects of TiO<sub>2</sub> Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines |
| title_full_unstemmed | The Effects of TiO<sub>2</sub> Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines |
| title_short | The Effects of TiO<sub>2</sub> Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines |
| title_sort | effects of tio sub 2 sub nanoparticles on cisplatin cytotoxicity in cancer cell lines |
| topic | titanium dioxide nanoparticles cisplatin cytotoxicity drug resistance p-glycoprotein |
| url | https://www.mdpi.com/1422-0067/21/2/605 |
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