In Vitro and In Vivo Toxicity Evaluation of Natural Products with Potential Applications as Biopesticides
The use of natural products in agriculture as pesticides has been strongly advocated. However, it is necessary to assess their toxicity to ensure their safe use. In the present study, mammalian cell lines and fish models of the zebrafish (<i>Danio rerio)</i> and medaka (<i>Oryzias...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/13/11/805 |
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| author | Felicia Sangermano Marco Masi Amrish Kumar Ravindra Peravali Angela Tuzi Alessio Cimmino Daniela Vallone Giuliana Giamundo Ivan Conte Antonio Evidente Viola Calabrò |
| author_facet | Felicia Sangermano Marco Masi Amrish Kumar Ravindra Peravali Angela Tuzi Alessio Cimmino Daniela Vallone Giuliana Giamundo Ivan Conte Antonio Evidente Viola Calabrò |
| author_sort | Felicia Sangermano |
| collection | DOAJ |
| description | The use of natural products in agriculture as pesticides has been strongly advocated. However, it is necessary to assess their toxicity to ensure their safe use. In the present study, mammalian cell lines and fish models of the zebrafish (<i>Danio rerio)</i> and medaka (<i>Oryzias latipes</i>) have been used to investigate the toxic effects of ten natural products which have potential applications as biopesticides. The fungal metabolites cavoxin, <i>epi</i>-epoformin, papyracillic acid, seiridin and sphaeropsidone, together with the plant compounds inuloxins A and C and ungeremine, showed no toxic effects in mammalian cells and zebrafish embryos. Conversely, cyclopaldic and α-costic acids, produced by <i>Seiridium cupressi</i> and <i>Dittrichia viscosa,</i> respectively, caused significant mortality in zebrafish and medaka embryos as a result of yolk coagulation. However, both compounds showed little effect in zebrafish or mammalian cell lines in culture, thus highlighting the importance of the fish embryotoxicity test in the assessment of environmental impact. Given the embryotoxicity of α-costic acid and cyclopaldic acid, their use as biopesticides is not recommended. Further ecotoxicological studies are needed to evaluate the potential applications of the other compounds. |
| first_indexed | 2024-03-10T05:00:33Z |
| format | Article |
| id | doaj.art-cf80545af7f34ffb9133ccd7dd50c254 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T05:00:33Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-cf80545af7f34ffb9133ccd7dd50c2542023-11-23T01:49:24ZengMDPI AGToxins2072-66512021-11-01131180510.3390/toxins13110805In Vitro and In Vivo Toxicity Evaluation of Natural Products with Potential Applications as BiopesticidesFelicia Sangermano0Marco Masi1Amrish Kumar2Ravindra Peravali3Angela Tuzi4Alessio Cimmino5Daniela Vallone6Giuliana Giamundo7Ivan Conte8Antonio Evidente9Viola Calabrò10Department of Biology, University of Naples Federico II, Complesso Universitario Monte Sant’Angelo, Via Cintia 4, 80126 Naples, ItalyDepartment of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte Sant’Angelo, Via Cintia 4, 80126 Naples, ItalyInstitute for Biological and Chemical Systems-Biological Information Processing (IBCS-BIP), Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, GermanyInstitute for Biological and Chemical Systems-Biological Information Processing (IBCS-BIP), Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, GermanyDepartment of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte Sant’Angelo, Via Cintia 4, 80126 Naples, ItalyDepartment of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte Sant’Angelo, Via Cintia 4, 80126 Naples, ItalyInstitute for Biological and Chemical Systems-Biological Information Processing (IBCS-BIP), Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, GermanyTelethon Institute of Genetics and Medicine, Via Campi Flegrei 34, 80078 Pozzuoli, ItalyDepartment of Biology, University of Naples Federico II, Complesso Universitario Monte Sant’Angelo, Via Cintia 4, 80126 Naples, ItalyDepartment of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte Sant’Angelo, Via Cintia 4, 80126 Naples, ItalyDepartment of Biology, University of Naples Federico II, Complesso Universitario Monte Sant’Angelo, Via Cintia 4, 80126 Naples, ItalyThe use of natural products in agriculture as pesticides has been strongly advocated. However, it is necessary to assess their toxicity to ensure their safe use. In the present study, mammalian cell lines and fish models of the zebrafish (<i>Danio rerio)</i> and medaka (<i>Oryzias latipes</i>) have been used to investigate the toxic effects of ten natural products which have potential applications as biopesticides. The fungal metabolites cavoxin, <i>epi</i>-epoformin, papyracillic acid, seiridin and sphaeropsidone, together with the plant compounds inuloxins A and C and ungeremine, showed no toxic effects in mammalian cells and zebrafish embryos. Conversely, cyclopaldic and α-costic acids, produced by <i>Seiridium cupressi</i> and <i>Dittrichia viscosa,</i> respectively, caused significant mortality in zebrafish and medaka embryos as a result of yolk coagulation. However, both compounds showed little effect in zebrafish or mammalian cell lines in culture, thus highlighting the importance of the fish embryotoxicity test in the assessment of environmental impact. Given the embryotoxicity of α-costic acid and cyclopaldic acid, their use as biopesticides is not recommended. Further ecotoxicological studies are needed to evaluate the potential applications of the other compounds.https://www.mdpi.com/2072-6651/13/11/805plant and microbial metabolitescyclopaldic and α-costic acidsagricultural applicationcytotoxicityDNA damagedevelopmental toxicity |
| spellingShingle | Felicia Sangermano Marco Masi Amrish Kumar Ravindra Peravali Angela Tuzi Alessio Cimmino Daniela Vallone Giuliana Giamundo Ivan Conte Antonio Evidente Viola Calabrò In Vitro and In Vivo Toxicity Evaluation of Natural Products with Potential Applications as Biopesticides Toxins plant and microbial metabolites cyclopaldic and α-costic acids agricultural application cytotoxicity DNA damage developmental toxicity |
| title | In Vitro and In Vivo Toxicity Evaluation of Natural Products with Potential Applications as Biopesticides |
| title_full | In Vitro and In Vivo Toxicity Evaluation of Natural Products with Potential Applications as Biopesticides |
| title_fullStr | In Vitro and In Vivo Toxicity Evaluation of Natural Products with Potential Applications as Biopesticides |
| title_full_unstemmed | In Vitro and In Vivo Toxicity Evaluation of Natural Products with Potential Applications as Biopesticides |
| title_short | In Vitro and In Vivo Toxicity Evaluation of Natural Products with Potential Applications as Biopesticides |
| title_sort | in vitro and in vivo toxicity evaluation of natural products with potential applications as biopesticides |
| topic | plant and microbial metabolites cyclopaldic and α-costic acids agricultural application cytotoxicity DNA damage developmental toxicity |
| url | https://www.mdpi.com/2072-6651/13/11/805 |
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