Genetic Diversity and Virulence Determinants of Escherichia coli Strains Isolated from Patients with Crohn's Disease in Spain and Chile
Adherent-invasive Escherichia coli (AIEC) strains are genetically variable and virulence factors for AIEC are non-specific. FimH is the most studied pathogenicity-related protein, and there have been few studies on other proteins, such as Serine Protease Autotransporters of Enterobacteriacea (SPATEs...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-05-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00639/full |
| _version_ | 1819295687959904256 |
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| author | Sandra Céspedes Waleska Saitz Felipe Del Canto Marjorie De la Fuente Rodrigo Quera Marcela Hermoso Rául Muñoz Daniel Ginard Sam Khorrami Jorge Girón Rodrigo Assar Ramón Rosselló-Mora Roberto M. Vidal |
| author_facet | Sandra Céspedes Waleska Saitz Felipe Del Canto Marjorie De la Fuente Rodrigo Quera Marcela Hermoso Rául Muñoz Daniel Ginard Sam Khorrami Jorge Girón Rodrigo Assar Ramón Rosselló-Mora Roberto M. Vidal |
| author_sort | Sandra Céspedes |
| collection | DOAJ |
| description | Adherent-invasive Escherichia coli (AIEC) strains are genetically variable and virulence factors for AIEC are non-specific. FimH is the most studied pathogenicity-related protein, and there have been few studies on other proteins, such as Serine Protease Autotransporters of Enterobacteriacea (SPATEs). The goal of this study is to characterize E. coli strains isolated from patients with Crohn's disease (CD) in Chile and Spain, and identify genetic differences between strains associated with virulence markers and clonality. We characterized virulence factors and genetic variability by pulse field electrophoresis (PFGE) in 50 E. coli strains isolated from Chilean and Spanish patients with CD, and also determined which of these strains presented an AIEC phenotype. Twenty-six E. coli strains from control patients were also included. PFGE patterns were heterogeneous and we also observed a highly diverse profile of virulence genes among all E. coli strains obtained from patients with CD, including those strains defined as AIEC. Two iron transporter genes chuA, and irp2, were detected in various combinations in 68–84% of CD strains. We found that the most significant individual E. coli genetic marker associated with CD E. coli strains was chuA. In addition, patho-adaptative fimH mutations were absent in some of the highly adherent and invasive strains. The fimH adhesin, the iron transporter irp2, and Class-2 SPATEs did not show a significant association with CD strains. The V27A fimH mutation was detected in the most CD strains. This study highlights the genetic variability of E. coli CD strains from two distinct geographic origins, most of them affiliated with the B2 or D E. coli phylogroups and also reveals that nearly 40% of Chilean and Spanish CD patients are colonized with E.coli with a characteristic AIEC phenotype. |
| first_indexed | 2024-12-24T04:46:11Z |
| format | Article |
| id | doaj.art-cf8212e13b744a0ab493e02e08f9bb8c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-24T04:46:11Z |
| publishDate | 2017-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-cf8212e13b744a0ab493e02e08f9bb8c2022-12-21T17:14:40ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-05-01810.3389/fmicb.2017.00639226949Genetic Diversity and Virulence Determinants of Escherichia coli Strains Isolated from Patients with Crohn's Disease in Spain and ChileSandra Céspedes0Waleska Saitz1Felipe Del Canto2Marjorie De la Fuente3Rodrigo Quera4Marcela Hermoso5Rául Muñoz6Daniel Ginard7Sam Khorrami8Jorge Girón9Rodrigo Assar10Ramón Rosselló-Mora11Roberto M. Vidal12Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de ChileSantiago, ChileInstituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de ChileSantiago, ChileInstituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de ChileSantiago, ChileGastroenterology Unit, Clínica Las CondesSantiago, ChileGastroenterology Unit, Clínica Las CondesSantiago, ChileInstituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de ChileSantiago, ChileInstitut Mediterrani d'Estudis Avançats (CSIC-UIB)Illes Balears, SpainDepartment of Gastroenterology and Palma Health Research Institute, Hospital Universitario Son EspasesPalma de Mallorca, SpainDepartment of Gastroenterology and Palma Health Research Institute, Hospital Universitario Son EspasesPalma de Mallorca, SpainDepartment of Pediatrics, University of Virginia School of MedicineCharlottesville, VA, USAInstituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de ChileSantiago, ChileInstitut Mediterrani d'Estudis Avançats (CSIC-UIB)Illes Balears, SpainInstituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de ChileSantiago, ChileAdherent-invasive Escherichia coli (AIEC) strains are genetically variable and virulence factors for AIEC are non-specific. FimH is the most studied pathogenicity-related protein, and there have been few studies on other proteins, such as Serine Protease Autotransporters of Enterobacteriacea (SPATEs). The goal of this study is to characterize E. coli strains isolated from patients with Crohn's disease (CD) in Chile and Spain, and identify genetic differences between strains associated with virulence markers and clonality. We characterized virulence factors and genetic variability by pulse field electrophoresis (PFGE) in 50 E. coli strains isolated from Chilean and Spanish patients with CD, and also determined which of these strains presented an AIEC phenotype. Twenty-six E. coli strains from control patients were also included. PFGE patterns were heterogeneous and we also observed a highly diverse profile of virulence genes among all E. coli strains obtained from patients with CD, including those strains defined as AIEC. Two iron transporter genes chuA, and irp2, were detected in various combinations in 68–84% of CD strains. We found that the most significant individual E. coli genetic marker associated with CD E. coli strains was chuA. In addition, patho-adaptative fimH mutations were absent in some of the highly adherent and invasive strains. The fimH adhesin, the iron transporter irp2, and Class-2 SPATEs did not show a significant association with CD strains. The V27A fimH mutation was detected in the most CD strains. This study highlights the genetic variability of E. coli CD strains from two distinct geographic origins, most of them affiliated with the B2 or D E. coli phylogroups and also reveals that nearly 40% of Chilean and Spanish CD patients are colonized with E.coli with a characteristic AIEC phenotype.http://journal.frontiersin.org/article/10.3389/fmicb.2017.00639/fulladherent invasive Escherichia coli (AIEC)clonal relationshipCrohn's diseasebiopsyvirulence genesfimH mutations |
| spellingShingle | Sandra Céspedes Waleska Saitz Felipe Del Canto Marjorie De la Fuente Rodrigo Quera Marcela Hermoso Rául Muñoz Daniel Ginard Sam Khorrami Jorge Girón Rodrigo Assar Ramón Rosselló-Mora Roberto M. Vidal Genetic Diversity and Virulence Determinants of Escherichia coli Strains Isolated from Patients with Crohn's Disease in Spain and Chile Frontiers in Microbiology adherent invasive Escherichia coli (AIEC) clonal relationship Crohn's disease biopsy virulence genes fimH mutations |
| title | Genetic Diversity and Virulence Determinants of Escherichia coli Strains Isolated from Patients with Crohn's Disease in Spain and Chile |
| title_full | Genetic Diversity and Virulence Determinants of Escherichia coli Strains Isolated from Patients with Crohn's Disease in Spain and Chile |
| title_fullStr | Genetic Diversity and Virulence Determinants of Escherichia coli Strains Isolated from Patients with Crohn's Disease in Spain and Chile |
| title_full_unstemmed | Genetic Diversity and Virulence Determinants of Escherichia coli Strains Isolated from Patients with Crohn's Disease in Spain and Chile |
| title_short | Genetic Diversity and Virulence Determinants of Escherichia coli Strains Isolated from Patients with Crohn's Disease in Spain and Chile |
| title_sort | genetic diversity and virulence determinants of escherichia coli strains isolated from patients with crohn s disease in spain and chile |
| topic | adherent invasive Escherichia coli (AIEC) clonal relationship Crohn's disease biopsy virulence genes fimH mutations |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00639/full |
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