DRD2 Dopamine Receptor and IL-1β Gene Expression Changes Involved in Wound Healing in Type 2 Diabetic Patients with Foot Ulcer
Background and purpose: Diabetic foot ulcer is a complication of type 2 diabetes. Various factors, such as changes in the level of cytokines (especially IL-1β) can interfere with the development of diabetic foot ulcer. Several factors affect the level of IL-1β levels, including the rate of neurotran...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2018-07-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-10624-en.html |
| _version_ | 1797943813803606016 |
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| author | Neda Ebrahimzadeh Hajar Vaseghi Garshasb Rigi Gholamreza Esmaeeli Djavid Shahla Mohammad Ganji Amirreza Farhoud Majid Pornour |
| author_facet | Neda Ebrahimzadeh Hajar Vaseghi Garshasb Rigi Gholamreza Esmaeeli Djavid Shahla Mohammad Ganji Amirreza Farhoud Majid Pornour |
| author_sort | Neda Ebrahimzadeh |
| collection | DOAJ |
| description | Background and purpose: Diabetic foot ulcer is a complication of type 2 diabetes. Various factors, such as changes in the level of cytokines (especially IL-1β) can interfere with the development of diabetic foot ulcer. Several factors affect the level of IL-1β levels, including the rate of neurotransmitter, especially dopamine and its receptors. The aim of this study was to investigate the changes in IL-1β and dopamine DRD2 receptor in peripheral blood mononuclear cells (PBMCs) in diabetic foot ulcers.
Materials and methods: In a cross sectional comparative study, peripheral blood samples were collected from 31 subjects with diabetic foot ulcer, 29 without ulcer, and 25 healthy individuals (control). Total mRNA was extracted from PBMCs and cDNA was synthesized to study the DRD2 and il-1β gene expression variations by real time PCR. Concentration of il-1β was also investigated in sera.
Results: Significant decrease was seen in gene expression and sera concentration of il-1β in PBMCs in diabetic patients with foot ulcer. There was also a significant reduction in the expression of DRD2 receptor gene in patients with and without foot ulcer.
Conclusion: Reduction of IL-1β expression seems to be related to changes in the expression of DRD2 receptor gene. Therefore, after supplementary studies, reducing the expression of DRD2 could be regarded as a prognostic factor and as an effective factor in the spread of diabetic foot ulcer. |
| first_indexed | 2024-04-10T20:30:03Z |
| format | Article |
| id | doaj.art-cf82b0cd2aca4a67be215a26bd5cbb61 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T20:30:03Z |
| publishDate | 2018-07-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-cf82b0cd2aca4a67be215a26bd5cbb612023-01-25T07:32:06ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792018-07-01281624758DRD2 Dopamine Receptor and IL-1β Gene Expression Changes Involved in Wound Healing in Type 2 Diabetic Patients with Foot UlcerNeda Ebrahimzadeh0Hajar Vaseghi1Garshasb Rigi2Gholamreza Esmaeeli Djavid3Shahla Mohammad Ganji4Amirreza Farhoud5Majid Pornour6 MSc Student in Genetics, Department of Biology, Islamic Azad University, Damghan Branch, Damghan, Iran Assistant Professor, Photo Healing and Regeneration Group, Medical Laser Research Center, ACECR, Tehran, Iran Assistant Professor, Department of Genetics, Faculty of Basic Science, University of Shahrekord, Shahrekord, Iran Assistant Professor, Photo Healing and Regeneration Group, Medical Laser Research Center, ACECR, Tehran, Iran Assistant Professor, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran Assistant Professor, Department of Orthopedics, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences (TUMS), Tehran, Iran Assistant Professor, Photo Healing and Regeneration Group, Medical Laser Research Center, ACECR, Tehran, Iran Background and purpose: Diabetic foot ulcer is a complication of type 2 diabetes. Various factors, such as changes in the level of cytokines (especially IL-1β) can interfere with the development of diabetic foot ulcer. Several factors affect the level of IL-1β levels, including the rate of neurotransmitter, especially dopamine and its receptors. The aim of this study was to investigate the changes in IL-1β and dopamine DRD2 receptor in peripheral blood mononuclear cells (PBMCs) in diabetic foot ulcers. Materials and methods: In a cross sectional comparative study, peripheral blood samples were collected from 31 subjects with diabetic foot ulcer, 29 without ulcer, and 25 healthy individuals (control). Total mRNA was extracted from PBMCs and cDNA was synthesized to study the DRD2 and il-1β gene expression variations by real time PCR. Concentration of il-1β was also investigated in sera. Results: Significant decrease was seen in gene expression and sera concentration of il-1β in PBMCs in diabetic patients with foot ulcer. There was also a significant reduction in the expression of DRD2 receptor gene in patients with and without foot ulcer. Conclusion: Reduction of IL-1β expression seems to be related to changes in the expression of DRD2 receptor gene. Therefore, after supplementary studies, reducing the expression of DRD2 could be regarded as a prognostic factor and as an effective factor in the spread of diabetic foot ulcer.http://jmums.mazums.ac.ir/article-1-10624-en.htmldiabetesfoot ulcerdopamine receptorgene expression changesil-1β |
| spellingShingle | Neda Ebrahimzadeh Hajar Vaseghi Garshasb Rigi Gholamreza Esmaeeli Djavid Shahla Mohammad Ganji Amirreza Farhoud Majid Pornour DRD2 Dopamine Receptor and IL-1β Gene Expression Changes Involved in Wound Healing in Type 2 Diabetic Patients with Foot Ulcer Journal of Mazandaran University of Medical Sciences diabetes foot ulcer dopamine receptor gene expression changes il-1β |
| title | DRD2 Dopamine Receptor and IL-1β Gene Expression Changes Involved in Wound Healing in Type 2 Diabetic Patients with Foot Ulcer |
| title_full | DRD2 Dopamine Receptor and IL-1β Gene Expression Changes Involved in Wound Healing in Type 2 Diabetic Patients with Foot Ulcer |
| title_fullStr | DRD2 Dopamine Receptor and IL-1β Gene Expression Changes Involved in Wound Healing in Type 2 Diabetic Patients with Foot Ulcer |
| title_full_unstemmed | DRD2 Dopamine Receptor and IL-1β Gene Expression Changes Involved in Wound Healing in Type 2 Diabetic Patients with Foot Ulcer |
| title_short | DRD2 Dopamine Receptor and IL-1β Gene Expression Changes Involved in Wound Healing in Type 2 Diabetic Patients with Foot Ulcer |
| title_sort | drd2 dopamine receptor and il 1β gene expression changes involved in wound healing in type 2 diabetic patients with foot ulcer |
| topic | diabetes foot ulcer dopamine receptor gene expression changes il-1β |
| url | http://jmums.mazums.ac.ir/article-1-10624-en.html |
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