Competitive Protein Binding Assay of Naproxen by Human Serum Albumin Functionalized Silicon Dioxide Nanoparticles
We have developed a new competitive protein binding assay (CPBA) based on human serum albumin functionalized silicon dioxide nanoparticles (nano-SiO<sub>2</sub>-HSA) that can be used for naproxen determination in urine. Compared with a conventional multi-well reaction plate, nano-SiO<...
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MDPI AG
2019-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/14/2593 |
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| author | Qian-Long Wang Jing Xie Jian Liang Geng-Ting Dong Li-Sheng Ding Pei Luo Lin-Sen Qing |
| author_facet | Qian-Long Wang Jing Xie Jian Liang Geng-Ting Dong Li-Sheng Ding Pei Luo Lin-Sen Qing |
| author_sort | Qian-Long Wang |
| collection | DOAJ |
| description | We have developed a new competitive protein binding assay (CPBA) based on human serum albumin functionalized silicon dioxide nanoparticles (nano-SiO<sub>2</sub>-HSA) that can be used for naproxen determination in urine. Compared with a conventional multi-well reaction plate, nano-SiO<sub>2</sub> with a high surface-area-to-volume ratio could be introduced as a stationary phase, markedly improving the analytical performance. Nano-SiO<sub>2</sub>-HSA and horseradish peroxidase-labeled-naproxen (HRP-naproxen) were prepared for the present CPBA method. In this study, a direct competitive binding to nano-SiO<sub>2</sub>-HSAwas performed between the free naproxen in the sample and HRP-naproxen. Thus, the catalytic color reactions were investigated on an HRP/3,3′5,5′-tetramethylbenzidine (TMB)/H<sub>2</sub>O<sub>2</sub> system by the HRP-naproxen/nano-SiO<sub>2</sub>-HSA composite for quantitative measurement via an ultraviolet spectrophotometer. A series of validation experiments indicated that our proposed methods can be applied satisfactorily to the determination of naproxen in urine samples. As a proof of principle, the newly developed nano-CPBA method for the quantification of naproxen in urine can be expected to have the advantages of low costs, fast speed, high accuracy, and relatively simple instrument requirements. Our method could be capable of expanding the analytical applications of nanomaterials and of determining other small-molecule compounds from various biological samples. |
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| language | English |
| last_indexed | 2024-12-10T10:36:32Z |
| publishDate | 2019-07-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-cf8407d868e94e7d971367b70556b7f92022-12-22T01:52:26ZengMDPI AGMolecules1420-30492019-07-012414259310.3390/molecules24142593molecules24142593Competitive Protein Binding Assay of Naproxen by Human Serum Albumin Functionalized Silicon Dioxide NanoparticlesQian-Long Wang0Jing Xie1Jian Liang2Geng-Ting Dong3Li-Sheng Ding4Pei Luo5Lin-Sen Qing6School of Pharmacy, Chengdu Medical College, Chengdu 610500, ChinaSchool of Pharmacy, Chengdu Medical College, Chengdu 610500, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, ChinaState Key Laboratory for Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, ChinaState Key Laboratory for Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, ChinaSchool of Pharmacy, Chengdu Medical College, Chengdu 610500, ChinaWe have developed a new competitive protein binding assay (CPBA) based on human serum albumin functionalized silicon dioxide nanoparticles (nano-SiO<sub>2</sub>-HSA) that can be used for naproxen determination in urine. Compared with a conventional multi-well reaction plate, nano-SiO<sub>2</sub> with a high surface-area-to-volume ratio could be introduced as a stationary phase, markedly improving the analytical performance. Nano-SiO<sub>2</sub>-HSA and horseradish peroxidase-labeled-naproxen (HRP-naproxen) were prepared for the present CPBA method. In this study, a direct competitive binding to nano-SiO<sub>2</sub>-HSAwas performed between the free naproxen in the sample and HRP-naproxen. Thus, the catalytic color reactions were investigated on an HRP/3,3′5,5′-tetramethylbenzidine (TMB)/H<sub>2</sub>O<sub>2</sub> system by the HRP-naproxen/nano-SiO<sub>2</sub>-HSA composite for quantitative measurement via an ultraviolet spectrophotometer. A series of validation experiments indicated that our proposed methods can be applied satisfactorily to the determination of naproxen in urine samples. As a proof of principle, the newly developed nano-CPBA method for the quantification of naproxen in urine can be expected to have the advantages of low costs, fast speed, high accuracy, and relatively simple instrument requirements. Our method could be capable of expanding the analytical applications of nanomaterials and of determining other small-molecule compounds from various biological samples.https://www.mdpi.com/1420-3049/24/14/2593competitive protein binding assaynaproxenhorseradish peroxidase labeled naproxen |
| spellingShingle | Qian-Long Wang Jing Xie Jian Liang Geng-Ting Dong Li-Sheng Ding Pei Luo Lin-Sen Qing Competitive Protein Binding Assay of Naproxen by Human Serum Albumin Functionalized Silicon Dioxide Nanoparticles Molecules competitive protein binding assay naproxen horseradish peroxidase labeled naproxen |
| title | Competitive Protein Binding Assay of Naproxen by Human Serum Albumin Functionalized Silicon Dioxide Nanoparticles |
| title_full | Competitive Protein Binding Assay of Naproxen by Human Serum Albumin Functionalized Silicon Dioxide Nanoparticles |
| title_fullStr | Competitive Protein Binding Assay of Naproxen by Human Serum Albumin Functionalized Silicon Dioxide Nanoparticles |
| title_full_unstemmed | Competitive Protein Binding Assay of Naproxen by Human Serum Albumin Functionalized Silicon Dioxide Nanoparticles |
| title_short | Competitive Protein Binding Assay of Naproxen by Human Serum Albumin Functionalized Silicon Dioxide Nanoparticles |
| title_sort | competitive protein binding assay of naproxen by human serum albumin functionalized silicon dioxide nanoparticles |
| topic | competitive protein binding assay naproxen horseradish peroxidase labeled naproxen |
| url | https://www.mdpi.com/1420-3049/24/14/2593 |
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