Size-Dependent Photodynamic Anticancer Activity of Biocompatible Multifunctional Magnetic Submicron Particles in Prostate Cancer Cells
In this study, newly designed biocompatible multifunctional magnetic submicron particles (CoFe2O4-HPs-FAs) of well-defined sizes (60, 133, 245, and 335 nm) were fabricated for application as a photosensitizer delivery agent for photodynamic therapy in cancer cells. To provide selective targeting of...
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MDPI AG
2016-09-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/21/9/1187 |
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| author | Kyong-Hoon Choi Ki Chang Nam Leszek Malkinski Eun Ha Choi Jin-Seung Jung Bong Joo Park |
| author_facet | Kyong-Hoon Choi Ki Chang Nam Leszek Malkinski Eun Ha Choi Jin-Seung Jung Bong Joo Park |
| author_sort | Kyong-Hoon Choi |
| collection | DOAJ |
| description | In this study, newly designed biocompatible multifunctional magnetic submicron particles (CoFe2O4-HPs-FAs) of well-defined sizes (60, 133, 245, and 335 nm) were fabricated for application as a photosensitizer delivery agent for photodynamic therapy in cancer cells. To provide selective targeting of cancer cells and destruction of cancer cell functionality, basic cobalt ferrite (CoFe2O4) particles were covalently bonded with a photosensitizer (PS), which comprises hematoporphyrin (HP), and folic acid (FA) molecules. The magnetic properties of the CoFe2O4 particles were finely adjusted by controlling the size of the primary CoFe2O4 nanograins, and secondary superstructured composite particles were formed by aggregation of the nanograins. The prepared CoFe2O4-HP-FA exhibited high water solubility, good MR-imaging capacity, and biocompatibility without any in vitro cytotoxicity. In particular, our CoFe2O4-HP-FA exhibited remarkable photodynamic anticancer efficiency via induction of apoptotic death in PC-3 prostate cancer cells in a particle size- and concentration-dependent manner. This size-dependent effect was determined by the specific surface area of the particles because the number of HP molecules increased with decreasing size and increasing surface area. These results indicate that our CoFe2O4-HP-FA may be applicable for photodynamic therapy (PDT) as a PS delivery material and a therapeutic agent for MR-imaging based PDT owing to their high saturation value for magnetization and superparamagnetism. |
| first_indexed | 2024-04-12T05:29:27Z |
| format | Article |
| id | doaj.art-cf89df8748404441b9b29c566925445b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T05:29:27Z |
| publishDate | 2016-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-cf89df8748404441b9b29c566925445b2022-12-22T03:46:10ZengMDPI AGMolecules1420-30492016-09-01219118710.3390/molecules21091187molecules21091187Size-Dependent Photodynamic Anticancer Activity of Biocompatible Multifunctional Magnetic Submicron Particles in Prostate Cancer CellsKyong-Hoon Choi0Ki Chang Nam1Leszek Malkinski2Eun Ha Choi3Jin-Seung Jung4Bong Joo Park5Plasma Bioscience Research Center, Kwangwoon University, 20 Kwangwoongil, Nowon-gu, Seoul 01897, KoreaDepartment of Medical Engineering, Dongguk University College of Medicine, Gyeonggi-do 10326, KoreaAdvanced Materials Research Institute, University of New Orleans, New Orleans, LA 70148, USAPlasma Bioscience Research Center, Kwangwoon University, 20 Kwangwoongil, Nowon-gu, Seoul 01897, KoreaDepartment of Chemistry, Gangneung-Wonju National University, Gangneung 25457, KoreaPlasma Bioscience Research Center, Kwangwoon University, 20 Kwangwoongil, Nowon-gu, Seoul 01897, KoreaIn this study, newly designed biocompatible multifunctional magnetic submicron particles (CoFe2O4-HPs-FAs) of well-defined sizes (60, 133, 245, and 335 nm) were fabricated for application as a photosensitizer delivery agent for photodynamic therapy in cancer cells. To provide selective targeting of cancer cells and destruction of cancer cell functionality, basic cobalt ferrite (CoFe2O4) particles were covalently bonded with a photosensitizer (PS), which comprises hematoporphyrin (HP), and folic acid (FA) molecules. The magnetic properties of the CoFe2O4 particles were finely adjusted by controlling the size of the primary CoFe2O4 nanograins, and secondary superstructured composite particles were formed by aggregation of the nanograins. The prepared CoFe2O4-HP-FA exhibited high water solubility, good MR-imaging capacity, and biocompatibility without any in vitro cytotoxicity. In particular, our CoFe2O4-HP-FA exhibited remarkable photodynamic anticancer efficiency via induction of apoptotic death in PC-3 prostate cancer cells in a particle size- and concentration-dependent manner. This size-dependent effect was determined by the specific surface area of the particles because the number of HP molecules increased with decreasing size and increasing surface area. These results indicate that our CoFe2O4-HP-FA may be applicable for photodynamic therapy (PDT) as a PS delivery material and a therapeutic agent for MR-imaging based PDT owing to their high saturation value for magnetization and superparamagnetism.http://www.mdpi.com/1420-3049/21/9/1187biocompatible multifunctional submicron particleshematoporphyrinfolic acidcytotoxicityphotodynamic anticancer therapymagnetic resonance imaging |
| spellingShingle | Kyong-Hoon Choi Ki Chang Nam Leszek Malkinski Eun Ha Choi Jin-Seung Jung Bong Joo Park Size-Dependent Photodynamic Anticancer Activity of Biocompatible Multifunctional Magnetic Submicron Particles in Prostate Cancer Cells Molecules biocompatible multifunctional submicron particles hematoporphyrin folic acid cytotoxicity photodynamic anticancer therapy magnetic resonance imaging |
| title | Size-Dependent Photodynamic Anticancer Activity of Biocompatible Multifunctional Magnetic Submicron Particles in Prostate Cancer Cells |
| title_full | Size-Dependent Photodynamic Anticancer Activity of Biocompatible Multifunctional Magnetic Submicron Particles in Prostate Cancer Cells |
| title_fullStr | Size-Dependent Photodynamic Anticancer Activity of Biocompatible Multifunctional Magnetic Submicron Particles in Prostate Cancer Cells |
| title_full_unstemmed | Size-Dependent Photodynamic Anticancer Activity of Biocompatible Multifunctional Magnetic Submicron Particles in Prostate Cancer Cells |
| title_short | Size-Dependent Photodynamic Anticancer Activity of Biocompatible Multifunctional Magnetic Submicron Particles in Prostate Cancer Cells |
| title_sort | size dependent photodynamic anticancer activity of biocompatible multifunctional magnetic submicron particles in prostate cancer cells |
| topic | biocompatible multifunctional submicron particles hematoporphyrin folic acid cytotoxicity photodynamic anticancer therapy magnetic resonance imaging |
| url | http://www.mdpi.com/1420-3049/21/9/1187 |
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