Three combinations of clonidine in association with tiletamine-zolazepam for anaesthesia induction in rats: evaluation of reflexes and pain sensibility
The aim of this study was to assess the combination of tiletamine-zolazepam (Zoletil 20®) with three different doses of clonidine for general anaesthesia induction in rats submitted to vascular microsurgery. The evaluation of anaesthetic and analgesic effects was performed in 30 Wistar rats randomly...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2012-10-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-201210-0003_three-combinations-of-clonidine-in-association-with-tiletamine-zolazepam-for-anaesthesia-induction-in-rats-eva.php |
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| author | G. Spinella J.M. Vilar C. Anastasi A. Santana U. Prati L. Roveda G. Ricciardi D. Britti |
| author_facet | G. Spinella J.M. Vilar C. Anastasi A. Santana U. Prati L. Roveda G. Ricciardi D. Britti |
| author_sort | G. Spinella |
| collection | DOAJ |
| description | The aim of this study was to assess the combination of tiletamine-zolazepam (Zoletil 20®) with three different doses of clonidine for general anaesthesia induction in rats submitted to vascular microsurgery. The evaluation of anaesthetic and analgesic effects was performed in 30 Wistar rats randomly divided into three groups and induced with Zoletil 20 [90 mg/kg Intraperitoneal (IP)] associated with three different doses of clonidine (60-90-120 μg/kg IP). Four clinical parameters were evaluated after induction: loss of righting reflex, voluntary movement, the pedal withdrawal response, and pain sensitivity tested by pinching the tail. The combination of Zoletil with 90 and 120 μg/kg of clonidine provided a surgical anaesthesia; however, 90 μg/kg of clonidine provided the most rapid anaesthesia induction, as confirmed by data obtained by clinical evaluation of the loss of the pedal withdrawal response and the absence of the tail pinch response. The increase in dose of clonidine did not lead to a more rapid action of the α2 agonist, probably due to achievement of a dose-dependent plateau. |
| first_indexed | 2024-04-10T08:01:15Z |
| format | Article |
| id | doaj.art-cf8ad2f814ce48dca41439b67df5e3bf |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T08:01:15Z |
| publishDate | 2012-10-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-cf8ad2f814ce48dca41439b67df5e3bf2023-02-23T03:50:02ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922012-10-01571053654210.17221/6365-VETMEDvet-201210-0003Three combinations of clonidine in association with tiletamine-zolazepam for anaesthesia induction in rats: evaluation of reflexes and pain sensibilityG. Spinella0J.M. Vilar1C. Anastasi2A. Santana3U. Prati4L. Roveda5G. Ricciardi6D. Britti7University of Catanzaro, Germaneto, ItalyFaculty of Veterinary Medicine, University of Las Palmas de Gran Canaria, Gran Canaria, SpainVeterinary practitioner, Catanzara, ItalyFaculty of Veterinary Medicine, University of Las Palmas de Gran Canaria, Gran Canaria, SpainUniversity of Catanzaro, Germaneto, ItalyUniversity of Catanzaro, Germaneto, ItalyVeterinary Practitioner, Vicenza, ItalyUniversity of Catanzaro, Germaneto, ItalyThe aim of this study was to assess the combination of tiletamine-zolazepam (Zoletil 20®) with three different doses of clonidine for general anaesthesia induction in rats submitted to vascular microsurgery. The evaluation of anaesthetic and analgesic effects was performed in 30 Wistar rats randomly divided into three groups and induced with Zoletil 20 [90 mg/kg Intraperitoneal (IP)] associated with three different doses of clonidine (60-90-120 μg/kg IP). Four clinical parameters were evaluated after induction: loss of righting reflex, voluntary movement, the pedal withdrawal response, and pain sensitivity tested by pinching the tail. The combination of Zoletil with 90 and 120 μg/kg of clonidine provided a surgical anaesthesia; however, 90 μg/kg of clonidine provided the most rapid anaesthesia induction, as confirmed by data obtained by clinical evaluation of the loss of the pedal withdrawal response and the absence of the tail pinch response. The increase in dose of clonidine did not lead to a more rapid action of the α2 agonist, probably due to achievement of a dose-dependent plateau.https://vetmed.agriculturejournals.cz/artkey/vet-201210-0003_three-combinations-of-clonidine-in-association-with-tiletamine-zolazepam-for-anaesthesia-induction-in-rats-eva.phpanaesthesia induction; clonidine; reflex testtiletamine-zolazepamwinstar rats |
| spellingShingle | G. Spinella J.M. Vilar C. Anastasi A. Santana U. Prati L. Roveda G. Ricciardi D. Britti Three combinations of clonidine in association with tiletamine-zolazepam for anaesthesia induction in rats: evaluation of reflexes and pain sensibility Veterinární Medicína anaesthesia induction; clonidine; reflex test tiletamine-zolazepam winstar rats |
| title | Three combinations of clonidine in association with tiletamine-zolazepam for anaesthesia induction in rats: evaluation of reflexes and pain sensibility |
| title_full | Three combinations of clonidine in association with tiletamine-zolazepam for anaesthesia induction in rats: evaluation of reflexes and pain sensibility |
| title_fullStr | Three combinations of clonidine in association with tiletamine-zolazepam for anaesthesia induction in rats: evaluation of reflexes and pain sensibility |
| title_full_unstemmed | Three combinations of clonidine in association with tiletamine-zolazepam for anaesthesia induction in rats: evaluation of reflexes and pain sensibility |
| title_short | Three combinations of clonidine in association with tiletamine-zolazepam for anaesthesia induction in rats: evaluation of reflexes and pain sensibility |
| title_sort | three combinations of clonidine in association with tiletamine zolazepam for anaesthesia induction in rats evaluation of reflexes and pain sensibility |
| topic | anaesthesia induction; clonidine; reflex test tiletamine-zolazepam winstar rats |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-201210-0003_three-combinations-of-clonidine-in-association-with-tiletamine-zolazepam-for-anaesthesia-induction-in-rats-eva.php |
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